##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AviII AvrII
BfrI BglII BlnI BsiWI BsmI
BspDI BspLU11I Bst1107I BstBI BstZ17I
ClaI DraI EcoRI EcoT22I FspI
HindIII HpaI KpnI MfeI MunI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I SalI ScaI SfuI
SpeI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AatII 6080 GACGTC GACGT^C
AhdI 7865 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 7865 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2363 CACGTG CAC^GTG
BciVI 7526 GTATCC GTATCC(6/5)
BclI 7320 TGATCA T^GATCA
BsaBI 3217 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 6582 TCATGA T^CATGA
Eam1105I 7865 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 911 GATATC GAT^ATC
FbaI 7320 TGATCA T^GATCA
FseI 3850 GGCCGGCC GGCCGG^CC
KspI 3419 CCGCGG CCGC^GG
MamI 3217 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 8433 TTAA T^TAA
NdeI 7213 CATATG CA^TATG
PmaCI 2363 CACGTG CAC^GTG
PmlI 2363 CACGTG CAC^GTG
PvuI 6345 CGATCG CGAT^CG
RcaI 6582 TCATGA T^CATGA
SacII 3419 CCGCGG CCGC^GG
SgrAI 5900 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 7139 TACGTA TAC^GTA
Tru9I 8433 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 5871 TCCGGA T^CCGGA
6663 TCCGGA
AcsI 5423 AAATTC [GA]^AATT[CT]
8344 AAATTT
Aor51HI 1213 AGCGCT AGC^GCT
2007 AGCGCT
ApoI 5423 AAATTC [GA]^AATT[CT]
8344 AAATTT
BamHI 4003 GGATCC G^GATCC
5868 GGATCC
BsaAI 2363 CACGTG [CT]AC^GT[GA]
7139 TACGTA
BseAI 5871 TCCGGA T^CCGGA
6663 TCCGGA
Bsp1407I 3309 TGTACA T^GTACA
8364 TGTACA
BspEI 5871 TCCGGA T^CCGGA
6663 TCCGGA
BsrGI 3309 TGTACA T^GTACA
8364 TGTACA
BssHII 3502 GCGCGC G^CGCGC
7450 GCGCGC
CpoI 260 CGGTCCG CG^G[AT]CCG
2138 CGGACCG
DraIII 1612 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2375 CACGCGGTG
EagI 948 CGGCCG C^GGCCG
2646 CGGCCG
EclXI 948 CGGCCG C^GGCCG
2646 CGGCCG
Eco47III 1213 AGCGCT AGC^GCT
2007 AGCGCT
Eco52I 948 CGGCCG C^GGCCG
2646 CGGCCG
MluI 4236 ACGCGT A^CGCGT
7624 ACGCGT
MroI 5871 TCCGGA T^CCGGA
6663 TCCGGA
RsrII 260 CGGTCCG CG^G[AT]CCG
2138 CGGACCG
SbfI 3511 CCTGCAGG CCTGCA^GG
3666 CCTGCAGG
SphI 1467 GCATGC GCATG^C
6713 GCATGC
Sse8387I 3511 CCTGCAGG CCTGCA^GG
3666 CCTGCAGG
SspBI 3309 TGTACA T^GTACA
8364 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 160 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfiI SfoI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI