##################################
# NO CUT ENZYMES (   50 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AscI        
AseI        AsnI        Asp718I     AviII       AvrII       
BfrI        BglII       BlnI        BsiWI       BsmI        
BspDI       BspLU11I    Bst1107I    BstBI       BstZ17I     
ClaI        DraI        EcoRI       EcoT22I     FspI        
HindIII     HpaI        KpnI        MfeI        MunI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    SalI        ScaI        SfuI        
SpeI        SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AatII        6080  GACGTC               GACGT^C
AhdI         7865  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        7865  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        2363  CACGTG               CAC^GTG
BciVI        7526  GTATCC               GTATCC(6/5)
BclI         7320  TGATCA               T^GATCA
BsaBI        3217  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI        6582  TCATGA               T^CATGA
Eam1105I     7865  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV         911  GATATC               GAT^ATC
FbaI         7320  TGATCA               T^GATCA
FseI         3850  GGCCGGCC             GGCCGG^CC
KspI         3419  CCGCGG               CCGC^GG
MamI         3217  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI         8433  TTAA                 T^TAA
NdeI         7213  CATATG               CA^TATG
PmaCI        2363  CACGTG               CAC^GTG
PmlI         2363  CACGTG               CAC^GTG
PvuI         6345  CGATCG               CGAT^CG
RcaI         6582  TCATGA               T^CATGA
SacII        3419  CCGCGG               CCGC^GG
SgrAI        5900  CGCCGGTG             C[GA]^CCGG[CT]G
SnaBI        7139  TACGTA               TAC^GTA
Tru9I        8433  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccIII       5871  TCCGGA               T^CCGGA
             6663  TCCGGA              
AcsI         5423  AAATTC               [GA]^AATT[CT]
             8344  AAATTT              
Aor51HI      1213  AGCGCT               AGC^GCT
             2007  AGCGCT              
ApoI         5423  AAATTC               [GA]^AATT[CT]
             8344  AAATTT              
BamHI        4003  GGATCC               G^GATCC
             5868  GGATCC              
BsaAI        2363  CACGTG               [CT]AC^GT[GA]
             7139  TACGTA              
BseAI        5871  TCCGGA               T^CCGGA
             6663  TCCGGA              
Bsp1407I     3309  TGTACA               T^GTACA
             8364  TGTACA              
BspEI        5871  TCCGGA               T^CCGGA
             6663  TCCGGA              
BsrGI        3309  TGTACA               T^GTACA
             8364  TGTACA              
BssHII       3502  GCGCGC               G^CGCGC
             7450  GCGCGC              
CpoI          260  CGGTCCG              CG^G[AT]CCG
             2138  CGGACCG             
DraIII       1612  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2375  CACGCGGTG           
EagI          948  CGGCCG               C^GGCCG
             2646  CGGCCG              
EclXI         948  CGGCCG               C^GGCCG
             2646  CGGCCG              
Eco47III     1213  AGCGCT               AGC^GCT
             2007  AGCGCT              
Eco52I        948  CGGCCG               C^GGCCG
             2646  CGGCCG              
MluI         4236  ACGCGT               A^CGCGT
             7624  ACGCGT              
MroI         5871  TCCGGA               T^CCGGA
             6663  TCCGGA              
RsrII         260  CGGTCCG              CG^G[AT]CCG
             2138  CGGACCG             
SbfI         3511  CCTGCAGG             CCTGCA^GG
             3666  CCTGCAGG            
SphI         1467  GCATGC               GCATG^C
             6713  GCATGC              
Sse8387I     3511  CCTGCAGG             CCTGCA^GG
             3666  CCTGCAGG            
SspBI        3309  TGTACA               T^GTACA
             8364  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  160 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       Asp700I     AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbsI        BbvCI       BbvI        
BcgI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrFI       BsrI        BssKI       
BssSI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DrdI        DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflFI       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SfiI        SfoI        SmaI        SmlI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI        XmnI