##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccI AccIII AclI AflII Aor51HI
AscI AseI AsnI AviII BamHI
BbeI BfrI BglI BlpI Bpu1102I
BseAI BsiEI BsiWI BspEI BspMI
BsrBI BssHII Bst1107I BstZ17I CelII
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoRV FseI FspI
HaeII HpaI KasI KspI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 2993 GACGTC GACGT^C
AccII 903 CGCG CG^CG
AcyI 2993 GACGTC G[GA]^CG[CT]C
AgeI 5034 ACCGGT A^CCGGT
AhdI 4433 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 938 GTGCAC G^TGCAC
AlwNI 1273 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3201 CCTCAGG CC^T[ACGT]AGG
ApaI 1087 GGGCCC GGGCC^C
ApaLI 938 GTGCAC G^TGCAC
AspEI 4433 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3915 CCTAGG C^CTAGG
BbrPI 845 CACGTG CAC^GTG
BbvCI 1080 CCTCAGC CCTCAGC(-5/-2)
BciVI 567 GGATAC GTATCC(6/5)
BglII 2045 AGATCT A^GATCT
BlnI 3915 CCTAGG C^CTAGG
BsaBI 3531 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2993 GACGTC G[GA]^CG[CT]C
BsaI 3355 GAGACC GGTCTC(1/5)
Bsp1407I 5668 TGTACA T^GTACA
BspDI 506 ATCGAT AT^CGAT
BspLU11I 4992 ACATGT A^CATGT
BsrGI 5668 TGTACA T^GTACA
BstAPI 3181 GCAAAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2671 TTCGAA TT^CGAA
BstEII 4861 GGTTACC G^GT[ACGT]ACC
BstPI 4861 GGTTACC G^GT[ACGT]ACC
BstUI 903 CGCG CG^CG
Bsu36I 3201 CCTCAGG CC^T[ACGT]AGG
CfoI 1017 GCGC GCG^C
ClaI 506 ATCGAT AT^CGAT
DraI 1700 TTTAAA TTT^AAA
Eam1105I 4433 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3201 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4861 GGTTACC G^GT[ACGT]ACC
EcoT22I 3250 ATGCAT ATGCA^T
HhaI 1017 GCGC GCG^C
Hin1I 2993 GACGTC G[GA]^CG[CT]C
HinP1I 1017 GCGC G^CGC
MamI 3531 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 903 CGCG CG^CG
NcoI 4273 CCATGG C^CATGG
NsiI 3250 ATGCAT ATGCA^T
NspV 2671 TTCGAA TT^CGAA
PacI 5647 TTAATTAA TTAAT^TAA
PflMI 3268 CCATTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 5034 ACCGGT A^CCGGT
PmaCI 845 CACGTG CAC^GTG
PmlI 845 CACGTG CAC^GTG
PshAI 4311 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 3330 GCTCTTC GCTCTTC(1/4)
ScaI 718 AGTACT AGT^ACT
SfuI 2671 TTCGAA TT^CGAA
SspBI 5668 TGTACA T^GTACA
Van91I 3268 CCATTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2384 CCAGAACCTCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 2893 GGTACC G^GTACC
3277 GGTACC
Asp718I 2893 GGTACC G^GTACC
3277 GGTACC
AspI 825 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2427 GACAGCGTC
BalI 95 TGGCCA TGG^CCA
4727 TGGCCA
BcgI 2486 CGATGCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4483 GCAAGCAGTTCG
BclI 28 TGATCA T^GATCA
4024 TGATCA
BsaAI 845 CACGTG [CT]AC^GT[GA]
3088 TACGTG
BsaWI 1197 ACCGGA [AT]^CCGG[AT]
5034 ACCGGT
BsgI 686 CTGCAC GTGCAG(16/14)
842 CTGCAC
BsmI 4843 GAATGC GAATGC(1/-1)
2668 GCATTC
BsrFI 2527 ACCGGC [GA]^CCGG[CT]
5034 ACCGGT
BssSI 3352 CACGAG CACGAG(-5/-1)
1134 CTCGTG
Cfr10I 2527 ACCGGC [GA]^CCGG[CT]
5034 ACCGGT
FauI 1085 GCGGG CCCGC(4/6)
5208 GCGGG
FbaI 28 TGATCA T^GATCA
4024 TGATCA
HgaI 2431 GCGTC GACGC(5/10)
4921 GCGTC
HincII 523 GTCAAC GT[CT]^[GA]AC
2721 GTCAAC
HindII 523 GTCAAC GT[CT]^[GA]AC
2721 GTCAAC
KpnI 2893 GGTACC GGTAC^C
3277 GGTACC
MluNI 95 TGGCCA TGG^CCA
4727 TGGCCA
MscI 95 TGGCCA TGG^CCA
4727 TGGCCA
NdeI 75 CATATG CA^TATG
4244 CATATG
PaeR7I 160 CTCGAG C^TCGAG
1321 CTCGAG
PflFI 825 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2427 GACAGCGTC
PpuMI 1567 GGGACCT [GA]G^G[AT]CC[CT]
2078 AGGTCCT
SexAI 419 ACCAGGT A^CC[AT]GGT
5326 ACCAGGT
SmaI 1264 CCCGGG CCC^GGG
1489 CCCGGG
SphI 384 GCATGC GCATG^C
896 GCATGC
Tth111I 825 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2427 GACAGCGTC
XbaI 1403 TCTAGA T^CTAGA
3017 TCTAGA
XhoI 160 CTCGAG C^TCGAG
1321 CTCGAG
XmaCI 1264 CCCGGG C^CCGGG
1489 CCCGGG
XmaI 1264 CCCGGG C^CCGGG
1489 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI