################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AccI AccIII AclI AflII Aor51HI AscI AseI AsnI AviII BamHI BbeI BfrI BglI BlpI Bpu1102I BseAI BsiEI BsiWI BspEI BspMI BsrBI BssHII Bst1107I BstZ17I CelII CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRV FseI FspI HaeII HpaI KasI KspI MluI MroI NaeI NarI NgoMIV NheI NotI NruI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 2993 GACGTC GACGT^C AccII 903 CGCG CG^CG AcyI 2993 GACGTC G[GA]^CG[CT]C AgeI 5034 ACCGGT A^CCGGT AhdI 4433 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 938 GTGCAC G^TGCAC AlwNI 1273 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 3201 CCTCAGG CC^T[ACGT]AGG ApaI 1087 GGGCCC GGGCC^C ApaLI 938 GTGCAC G^TGCAC AspEI 4433 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3915 CCTAGG C^CTAGG BbrPI 845 CACGTG CAC^GTG BbvCI 1080 CCTCAGC CCTCAGC(-5/-2) BciVI 567 GGATAC GTATCC(6/5) BglII 2045 AGATCT A^GATCT BlnI 3915 CCTAGG C^CTAGG BsaBI 3531 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2993 GACGTC G[GA]^CG[CT]C BsaI 3355 GAGACC GGTCTC(1/5) Bsp1407I 5668 TGTACA T^GTACA BspDI 506 ATCGAT AT^CGAT BspLU11I 4992 ACATGT A^CATGT BsrGI 5668 TGTACA T^GTACA BstAPI 3181 GCAAAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2671 TTCGAA TT^CGAA BstEII 4861 GGTTACC G^GT[ACGT]ACC BstPI 4861 GGTTACC G^GT[ACGT]ACC BstUI 903 CGCG CG^CG Bsu36I 3201 CCTCAGG CC^T[ACGT]AGG CfoI 1017 GCGC GCG^C ClaI 506 ATCGAT AT^CGAT DraI 1700 TTTAAA TTT^AAA Eam1105I 4433 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3201 CCTCAGG CC^T[ACGT]AGG EcoO65I 4861 GGTTACC G^GT[ACGT]ACC EcoT22I 3250 ATGCAT ATGCA^T HhaI 1017 GCGC GCG^C Hin1I 2993 GACGTC G[GA]^CG[CT]C HinP1I 1017 GCGC G^CGC MamI 3531 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 903 CGCG CG^CG NcoI 4273 CCATGG C^CATGG NsiI 3250 ATGCAT ATGCA^T NspV 2671 TTCGAA TT^CGAA PacI 5647 TTAATTAA TTAAT^TAA PflMI 3268 CCATTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 5034 ACCGGT A^CCGGT PmaCI 845 CACGTG CAC^GTG PmlI 845 CACGTG CAC^GTG PshAI 4311 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 3330 GCTCTTC GCTCTTC(1/4) ScaI 718 AGTACT AGT^ACT SfuI 2671 TTCGAA TT^CGAA SspBI 5668 TGTACA T^GTACA Van91I 3268 CCATTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2384 CCAGAACCTCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 2893 GGTACC G^GTACC 3277 GGTACC Asp718I 2893 GGTACC G^GTACC 3277 GGTACC AspI 825 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2427 GACAGCGTC BalI 95 TGGCCA TGG^CCA 4727 TGGCCA BcgI 2486 CGATGCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4483 GCAAGCAGTTCG BclI 28 TGATCA T^GATCA 4024 TGATCA BsaAI 845 CACGTG [CT]AC^GT[GA] 3088 TACGTG BsaWI 1197 ACCGGA [AT]^CCGG[AT] 5034 ACCGGT BsgI 686 CTGCAC GTGCAG(16/14) 842 CTGCAC BsmI 4843 GAATGC GAATGC(1/-1) 2668 GCATTC BsrFI 2527 ACCGGC [GA]^CCGG[CT] 5034 ACCGGT BssSI 3352 CACGAG CACGAG(-5/-1) 1134 CTCGTG Cfr10I 2527 ACCGGC [GA]^CCGG[CT] 5034 ACCGGT FauI 1085 GCGGG CCCGC(4/6) 5208 GCGGG FbaI 28 TGATCA T^GATCA 4024 TGATCA HgaI 2431 GCGTC GACGC(5/10) 4921 GCGTC HincII 523 GTCAAC GT[CT]^[GA]AC 2721 GTCAAC HindII 523 GTCAAC GT[CT]^[GA]AC 2721 GTCAAC KpnI 2893 GGTACC GGTAC^C 3277 GGTACC MluNI 95 TGGCCA TGG^CCA 4727 TGGCCA MscI 95 TGGCCA TGG^CCA 4727 TGGCCA NdeI 75 CATATG CA^TATG 4244 CATATG PaeR7I 160 CTCGAG C^TCGAG 1321 CTCGAG PflFI 825 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2427 GACAGCGTC PpuMI 1567 GGGACCT [GA]G^G[AT]CC[CT] 2078 AGGTCCT SexAI 419 ACCAGGT A^CC[AT]GGT 5326 ACCAGGT SmaI 1264 CCCGGG CCC^GGG 1489 CCCGGG SphI 384 GCATGC GCATG^C 896 GCATGC Tth111I 825 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2427 GACAGCGTC XbaI 1403 TCTAGA T^CTAGA 3017 TCTAGA XhoI 160 CTCGAG C^TCGAG 1321 CTCGAG XmaCI 1264 CCCGGG C^CCGGG 1489 CCCGGG XmaI 1264 CCCGGG C^CCGGG 1489 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI