##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AclI AflII Asp700I AviII
BfrI BsaBI BsiEI BsiWI Bsp1407I
BspDI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco52I
FseI FspI HincII HindII HpaI
MamI MfeI MluI MunI NheI
NotI NruI NspV PacI PaeR7I
PmeI Psp1406I PvuI RsrII SalI
SapI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 6874 GTAGAC GT^[AC][GT]AC
AscI 486 GGCGCGCC GG^CGCGCC
AseI 3059 ATTAAT AT^TAAT
AsnI 3059 ATTAAT AT^TAAT
AspI 1927 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 4378 GGCGCC GGCGC^C
BciVI 444 GTATCC GTATCC(6/5)
BclI 2861 TGATCA T^GATCA
BglII 7087 AGATCT A^GATCT
BspLU11I 4959 ACATGT A^CATGT
BssHII 487 GCGCGC G^CGCGC
EcoT22I 3680 ATGCAT ATGCA^T
FbaI 2861 TGATCA T^GATCA
HindIII 4122 AAGCTT A^AGCTT
KasI 4378 GGCGCC G^GCGCC
KspI 260 CCGCGG CCGC^GG
NaeI 217 GCCGGC GCC^GGC
NarI 4378 GGCGCC GG^CGCC
NgoMIV 217 GCCGGC G^CCGGC
NsiI 3680 ATGCAT ATGCA^T
PflFI 1927 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 6595 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3059 ATTAAT AT^TAAT
SacII 260 CCGCGG CCGC^GG
SbfI 7199 CCTGCAGG CCTGCA^GG
ScaI 5491 AGTACT AGT^ACT
SfoI 4378 GGCGCC GGC^GCC
Sse8387I 7199 CCTGCAGG CCTGCA^GG
Tth111I 1927 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3734 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 131 GGTACC G^GTACC
7348 GGTACC
AccIII 1747 TCCGGA T^CCGGA
4194 TCCGGA
AcyI 1969 GGCGTC G[GA]^CG[CT]C
4378 GGCGCC
AflIII 4959 ACATGT A^C[GA][CT]GT
7510 ACACGT
AgeI 2204 ACCGGT A^CCGGT
7075 ACCGGT
AhdI 6627 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7809 GACGGTAGGTC
Aor51HI 6471 AGCGCT AGC^GCT
7654 AGCGCT
Asp718I 131 GGTACC G^GTACC
7348 GGTACC
AspEI 6627 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7809 GACGGTAGGTC
BbrPI 1718 CACGTG CAC^GTG
7356 CACGTG
BcgI 2350 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5148 CGACGAGGGTGC
BsaHI 1969 GGCGTC G[GA]^CG[CT]C
4378 GGCGCC
BseAI 1747 TCCGGA T^CCGGA
4194 TCCGGA
BsmI 4278 GCATTC GAATGC(1/-1)
6241 GCATTC
BspEI 1747 TCCGGA T^CCGGA
4194 TCCGGA
BsrBI 233 CCGCTC CCGCTC(-3/-3)
5481 CCGCTC
BssSI 4325 CACGAG CACGAG(-5/-1)
3400 CTCGTG
DrdI 3197 GACCTGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3650 GACTGCAGTGTC
Eam1105I 6627 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7809 GACGGTAGGTC
Eco47III 6471 AGCGCT AGC^GCT
7654 AGCGCT
EcoRV 4456 GATATC GAT^ATC
5131 GATATC
Hin1I 1969 GGCGTC G[GA]^CG[CT]C
4378 GGCGCC
KpnI 131 GGTACC GGTAC^C
7348 GGTACC
MroI 1747 TCCGGA T^CCGGA
4194 TCCGGA
PinAI 2204 ACCGGT A^CCGGT
7075 ACCGGT
PmaCI 1718 CACGTG CAC^GTG
7356 CACGTG
PmlI 1718 CACGTG CAC^GTG
7356 CACGTG
SexAI 4725 ACCAGGT A^CC[AT]GGT
5261 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI AspHI AvaI AvaII
AvrII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI