################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AatII AclI AflII Asp700I AviII BfrI BsaBI BsiEI BsiWI Bsp1407I BspDI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI EagI EclXI Eco52I FseI FspI HincII HindII HpaI MamI MfeI MluI MunI NheI NotI NruI NspV PacI PaeR7I PmeI Psp1406I PvuI RsrII SalI SapI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccI 6874 GTAGAC GT^[AC][GT]AC AscI 486 GGCGCGCC GG^CGCGCC AseI 3059 ATTAAT AT^TAAT AsnI 3059 ATTAAT AT^TAAT AspI 1927 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 4378 GGCGCC GGCGC^C BciVI 444 GTATCC GTATCC(6/5) BclI 2861 TGATCA T^GATCA BglII 7087 AGATCT A^GATCT BspLU11I 4959 ACATGT A^CATGT BssHII 487 GCGCGC G^CGCGC EcoT22I 3680 ATGCAT ATGCA^T FbaI 2861 TGATCA T^GATCA HindIII 4122 AAGCTT A^AGCTT KasI 4378 GGCGCC G^GCGCC KspI 260 CCGCGG CCGC^GG NaeI 217 GCCGGC GCC^GGC NarI 4378 GGCGCC GG^CGCC NgoMIV 217 GCCGGC G^CCGGC NsiI 3680 ATGCAT ATGCA^T PflFI 1927 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 6595 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3059 ATTAAT AT^TAAT SacII 260 CCGCGG CCGC^GG SbfI 7199 CCTGCAGG CCTGCA^GG ScaI 5491 AGTACT AGT^ACT SfoI 4378 GGCGCC GGC^GCC Sse8387I 7199 CCTGCAGG CCTGCA^GG Tth111I 1927 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3734 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 131 GGTACC G^GTACC 7348 GGTACC AccIII 1747 TCCGGA T^CCGGA 4194 TCCGGA AcyI 1969 GGCGTC G[GA]^CG[CT]C 4378 GGCGCC AflIII 4959 ACATGT A^C[GA][CT]GT 7510 ACACGT AgeI 2204 ACCGGT A^CCGGT 7075 ACCGGT AhdI 6627 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 7809 GACGGTAGGTC Aor51HI 6471 AGCGCT AGC^GCT 7654 AGCGCT Asp718I 131 GGTACC G^GTACC 7348 GGTACC AspEI 6627 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 7809 GACGGTAGGTC BbrPI 1718 CACGTG CAC^GTG 7356 CACGTG BcgI 2350 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5148 CGACGAGGGTGC BsaHI 1969 GGCGTC G[GA]^CG[CT]C 4378 GGCGCC BseAI 1747 TCCGGA T^CCGGA 4194 TCCGGA BsmI 4278 GCATTC GAATGC(1/-1) 6241 GCATTC BspEI 1747 TCCGGA T^CCGGA 4194 TCCGGA BsrBI 233 CCGCTC CCGCTC(-3/-3) 5481 CCGCTC BssSI 4325 CACGAG CACGAG(-5/-1) 3400 CTCGTG DrdI 3197 GACCTGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3650 GACTGCAGTGTC Eam1105I 6627 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 7809 GACGGTAGGTC Eco47III 6471 AGCGCT AGC^GCT 7654 AGCGCT EcoRV 4456 GATATC GAT^ATC 5131 GATATC Hin1I 1969 GGCGTC G[GA]^CG[CT]C 4378 GGCGCC KpnI 131 GGTACC GGTAC^C 7348 GGTACC MroI 1747 TCCGGA T^CCGGA 4194 TCCGGA PinAI 2204 ACCGGT A^CCGGT 7075 ACCGGT PmaCI 1718 CACGTG CAC^GTG 7356 CACGTG PmlI 1718 CACGTG CAC^GTG 7356 CACGTG SexAI 4725 ACCAGGT A^CC[AT]GGT 5261 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 150 enzymes) # ######################################### AccII AciI AcsI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AspHI AvaI AvaII AvrII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI