##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AccIII AclI AflII AhdI AscI
AspEI AviII BfrI BseAI BsiWI
Bsp1407I BspEI BsrGI Bst1107I BstBI
BstZ17I CpoI Eam1105I EcoNI EcoRV
FseI FspI MroI NheI NotI
NruI NspV PacI PmeI Psp1406I
PvuI RsrII SbfI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
XbaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 6888 GTGCAC G^TGCAC
ApaLI 6888 GTGCAC G^TGCAC
BbeI 12 GGCGCC GGCGC^C
BglII 3860 AGATCT A^GATCT
BsaBI 5840 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 5243 ATCGAT AT^CGAT
BstEII 7507 GGTCACC G^GT[ACGT]ACC
BstPI 7507 GGTCACC G^GT[ACGT]ACC
ClaI 5243 ATCGAT AT^CGAT
EagI 3474 CGGCCG C^GGCCG
EclXI 3474 CGGCCG C^GGCCG
Eco52I 3474 CGGCCG C^GGCCG
EcoO65I 7507 GGTCACC G^GT[ACGT]ACC
HindIII 5953 AAGCTT A^AGCTT
HpaI 842 GTTAAC GTT^AAC
KasI 12 GGCGCC G^GCGCC
MamI 5840 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5823 CAATTG C^AATTG
MluI 559 ACGCGT A^CGCGT
MunI 5823 CAATTG C^AATTG
NaeI 3472 GCCGGC GCC^GGC
NarI 12 GGCGCC GG^CGCC
NgoMIV 3472 GCCGGC G^CCGGC
PaeR7I 238 CTCGAG C^TCGAG
PshAI 2801 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1881 GTCGAC G^TCGAC
SfoI 12 GGCGCC GGC^GCC
SgrAI 14 CGCCGGTG C[GA]^CCGG[CT]G
SwaI 6802 ATTTAAAT ATTT^AAAT
XhoI 238 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3032 GACGTC GACGT^C
4705 GACGTC
Acc65I 1961 GGTACC G^GTACC
3646 GGTACC
AgeI 277 ACCGGT A^CCGGT
1039 ACCGGT
AocI 84 CCTCAGG CC^T[ACGT]AGG
6450 CCTTAGG
Asp700I 609 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5377 GAAAAAGTTC
Asp718I 1961 GGTACC G^GTACC
3646 GGTACC
AspI 7502 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
8027 GACCCTGTC
AvrII 1253 CCTAGG C^CTAGG
7368 CCTAGG
BalI 1927 TGGCCA TGG^CCA
4346 TGGCCA
BcgI 2850 CGATACACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2419 GCATGGAGATCG
BciVI 3983 GGATAC GTATCC(6/5)
1703 GTATCC
BclI 1118 TGATCA T^GATCA
5238 TGATCA
BlnI 1253 CCTAGG C^CTAGG
7368 CCTAGG
BlpI 3587 GCTGAGC GC^T[ACGT]AGC
5073 GCTCAGC
Bpu1102I 3587 GCTGAGC GC^T[ACGT]AGC
5073 GCTCAGC
BsiEI 1037 CGACCG CG[GA][CT]^CG
3474 CGGCCG
BssHII 288 GCGCGC G^CGCGC
7908 GCGCGC
BssSI 306 CTCGTG CACGAG(-5/-1)
2468 CTCGTG
Bsu36I 84 CCTCAGG CC^T[ACGT]AGG
6450 CCTTAGG
CelII 3587 GCTGAGC GC^T[ACGT]AGC
5073 GCTCAGC
DraIII 5010 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
6399 CACAGTGTG
DrdI 2465 GACCTCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3032 GACGTCGTGGTC
Eco81I 84 CCTCAGG CC^T[ACGT]AGG
6450 CCTTAGG
EcoT22I 1063 ATGCAT ATGCA^T
1806 ATGCAT
FbaI 1118 TGATCA T^GATCA
5238 TGATCA
KpnI 1961 GGTACC GGTAC^C
3646 GGTACC
MluNI 1927 TGGCCA TGG^CCA
4346 TGGCCA
MscI 1927 TGGCCA TGG^CCA
4346 TGGCCA
NdeI 5511 CATATG CA^TATG
5745 CATATG
NsiI 1063 ATGCAT ATGCA^T
1806 ATGCAT
PflFI 7502 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
8027 GACCCTGTC
PinAI 277 ACCGGT A^CCGGT
1039 ACCGGT
ScaI 1539 AGTACT AGT^ACT
3880 AGTACT
SexAI 4460 ACCTGGT A^CC[AT]GGT
7503 ACCTGGT
SmaI 170 CCCGGG CCC^GGG
1414 CCCGGG
Tth111I 7502 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
8027 GACCCTGTC
XmaCI 170 CCCGGG C^CCGGG
1414 CCCGGG
XmaI 170 CCCGGG C^CCGGG
1414 CCCGGG
XmnI 609 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5377 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
Aor51HI ApaI ApoI AseI AsnI
AspHI AvaI AvaII BamHI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco47III EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PshBI PstI PvuII RcaI RsaI
SacI SacII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII