##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII ApoI AscI AspI BbrPI
BbsI BciVI BclI BfrI BglII
BpuAI BsaAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EcoRI EcoRV FbaI
FseI HpaI KspI MamI MfeI
MluI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI ScaI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 2437 GGTACC G^GTACC
AccIII 78 TCCGGA T^CCGGA
AgeI 1165 ACCGGT A^CCGGT
AhdI 1790 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 782 GTGCAC G^TGCAC
AocI 941 CCTCAGG CC^T[ACGT]AGG
ApaLI 782 GTGCAC G^TGCAC
AseI 17 ATTAAT AT^TAAT
AsnI 17 ATTAAT AT^TAAT
Asp700I 15 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2437 GGTACC G^GTACC
AspEI 1790 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1208 TGCGCA TGC^GCA
AvrII 2161 CCTAGG C^CTAGG
BamHI 2507 GGATCC G^GATCC
BbvCI 865 CCTCAGC CCTCAGC(-5/-2)
BlnI 2161 CCTAGG C^CTAGG
BseAI 78 TCCGGA T^CCGGA
BsiEI 1308 CGGCCG CG[GA][CT]^CG
BspEI 78 TCCGGA T^CCGGA
BspHI 390 TCATGA T^CATGA
BsrDI 1204 GCAATG GCAATG(2/0)
BssHII 1889 GCGCGC G^CGCGC
BssSI 599 CACGAG CACGAG(-5/-1)
BstEII 1234 GGTCACC G^GT[ACGT]ACC
BstPI 1234 GGTCACC G^GT[ACGT]ACC
Bsu36I 941 CCTCAGG CC^T[ACGT]AGG
DraIII 785 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1308 CGGCCG C^GGCCG
Eam1105I 1790 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1308 CGGCCG C^GGCCG
Eco52I 1308 CGGCCG C^GGCCG
Eco81I 941 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1234 GGTCACC G^GT[ACGT]ACC
EcoT22I 40 ATGCAT ATGCA^T
FspI 1208 TGCGCA TGC^GCA
HincII 1010 GTCAAC GT[CT]^[GA]AC
HindII 1010 GTCAAC GT[CT]^[GA]AC
HindIII 2167 AAGCTT A^AGCTT
KpnI 2437 GGTACC GGTAC^C
MaeII 780 ACGT A^CGT
MroI 78 TCCGGA T^CCGGA
MslI 2486 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 40 ATGCAT ATGCA^T
PinAI 1165 ACCGGT A^CCGGT
PshBI 17 ATTAAT AT^TAAT
PstI 728 CTGCAG CTGCA^G
RcaI 390 TCATGA T^CATGA
SapI 1153 GCTCTTC GCTCTTC(1/4)
SbfI 727 CCTGCAGG CCTGCA^GG
SexAI 714 ACCTGGT A^CC[AT]GGT
SfcI 728 CTGCAG C^T[GA][CT]AG
SfiI 265 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1878 CCCGGG CCC^GGG
SmlI 1550 CTCAAG C^T[CT][GA]AG
SphI 696 GCATGC GCATG^C
Sse8387I 727 CCTGCAGG CCTGCA^GG
StuI 1902 AGGCCT AGG^CCT
XcmI 2404 CCATGCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1878 CCCGGG C^CCGGG
XmaI 1878 CCCGGG C^CCGGG
XmnI 15 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Aor51HI 408 AGCGCT AGC^GCT
2449 AGCGCT
BalI 1632 TGGCCA TGG^CCA
2482 TGGCCA
BbeI 625 GGCGCC GGCGC^C
2499 GGCGCC
BcgI 1717 CGACTCGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1101 GCACTTCCCTCG
BglI 266 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1634 GCCAGTATGGC
BlpI 244 GCTGAGC GC^T[ACGT]AGC
2452 GCTTAGC
Bpu1102I 244 GCTGAGC GC^T[ACGT]AGC
2452 GCTTAGC
BsaI 1542 GAGACC GGTCTC(1/5)
2247 GGTCTC
BsaWI 78 TCCGGA [AT]^CCGG[AT]
1165 ACCGGT
BstXI 1794 CCAAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2485 CCACAGAAATGG
CelII 244 GCTGAGC GC^T[ACGT]AGC
2452 GCTTAGC
EarI 1154 CTCTTC CTCTTC(1/4)
1291 CTCTTC
Eco47III 408 AGCGCT AGC^GCT
2449 AGCGCT
HgaI 117 GACGC GACGC(5/10)
2274 GCGTC
KasI 625 GGCGCC G^GCGCC
2499 GGCGCC
Ksp632I 1154 CTCTTC CTCTTC(1/4)
1291 CTCTTC
MluNI 1632 TGGCCA TGG^CCA
2482 TGGCCA
MscI 1632 TGGCCA TGG^CCA
2482 TGGCCA
MseI 18 TTAA T^TAA
2545 TTAA
NarI 625 GGCGCC GG^CGCC
2499 GGCGCC
NspI 240 ACATGC [GA]CATG^[CT]
696 GCATGC
PflMI 1027 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1813 CCACAGCATGG
SfoI 625 GGCGCC GGC^GCC
2499 GGCGCC
Tru9I 18 TTAA T^TAA
2545 TTAA
Van91I 1027 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1813 CCACAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII