##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AgeI ApaI
AscI AseI AsnI Asp700I Asp718I
AvrII BbeI BciVI BclI BglII
BlnI BlpI Bpu1102I BsaBI BsiWI
BsmBI BspDI BspHI BsrBI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI EcoNI EcoO65I
EcoRI EcoRV EcoT14I FbaI FseI
HpaI KasI KpnI MamI MfeI
MunI NarI NcoI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspI StyI SwaI XbaI XcmI
XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccI 1669 GTCGAC GT^[AC][GT]AC
AcsI 2332 GAATTT [GA]^AATT[CT]
AcyI 1160 GGCGTC G[GA]^CG[CT]C
AflII 2491 CTTAAG C^TTAAG
AhdI 704 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1486 CCTGAGG CC^T[ACGT]AGG
ApoI 2332 GAATTT [GA]^AATT[CT]
AspEI 704 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1648 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 328 TGGCCA TGG^CCA
BbrPI 2066 CACGTG CAC^GTG
BbvCI 1277 GCTGAGG CCTCAGC(-5/-2)
BfrI 2491 CTTAAG C^TTAAG
BglI 2133 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2066 CACGTG [CT]AC^GT[GA]
BsaHI 1160 GGCGTC G[GA]^CG[CT]C
BsiEI 552 CGGCCG CG[GA][CT]^CG
BsmI 258 GCATTC GAATGC(1/-1)
Bsp1407I 501 TGTACA T^GTACA
BspLU11I 1815 ACATGT A^CATGT
BsrFI 398 GCCGGC [GA]^CCGG[CT]
BsrGI 501 TGTACA T^GTACA
BssHII 373 GCGCGC G^CGCGC
BssSI 836 CACGAG CACGAG(-5/-1)
Bsu36I 1486 CCTGAGG CC^T[ACGT]AGG
Cfr10I 398 GCCGGC [GA]^CCGG[CT]
DraI 2579 TTTAAA TTT^AAA
DraII 2093 GGGACCC [GA]G^G[ACGT]CC[CT]
EagI 552 CGGCCG C^GGCCG
Eam1105I 704 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 552 CGGCCG C^GGCCG
Eco52I 552 CGGCCG C^GGCCG
Eco81I 1486 CCTGAGG CC^T[ACGT]AGG
EcoO109I 2093 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 2234 ATGCAT ATGCA^T
Hin1I 1160 GGCGTC G[GA]^CG[CT]C
HindIII 1147 AAGCTT A^AGCTT
MluI 771 ACGCGT A^CGCGT
MluNI 328 TGGCCA TGG^CCA
MscI 328 TGGCCA TGG^CCA
NaeI 398 GCCGGC GCC^GGC
NgoMIV 398 GCCGGC G^CCGGC
NsiI 2234 ATGCAT ATGCA^T
PaeR7I 299 CTCGAG C^TCGAG
PflFI 1648 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2066 CACGTG CAC^GTG
PmlI 2066 CACGTG CAC^GTG
PpuMI 2093 GGGACCC [GA]G^G[AT]CC[CT]
SacI 1966 GAGCTC GAGCT^C
SalI 1669 GTCGAC G^TCGAC
SapI 1963 GAAGAGC GCTCTTC(1/4)
SbfI 2102 CCTGCAGG CCTGCA^GG
SmaI 631 CCCGGG CCC^GGG
SphI 366 GCATGC GCATG^C
Sse8387I 2102 CCTGCAGG CCTGCA^GG
SspBI 501 TGTACA T^GTACA
StuI 2495 AGGCCT AGG^CCT
TfiI 1082 GAATC G^A[AT]TC
Tth111I 1648 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 299 CTCGAG C^TCGAG
XmaCI 631 CCCGGG C^CCGGG
XmaI 631 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 1324 TCCGGA T^CCGGA
2440 TCCGGA
AviII 351 TGCGCA TGC^GCA
981 TGCGCA
BamHI 104 GGATCC G^GATCC
636 GGATCC
BanII 1133 GAGCCC G[GA]GC[CT]^C
1966 GAGCTC
BbsI 875 GAAGAC GAAGAC(2/6)
1100 GAAGAC
BcgI 1255 CGAGCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
594 GCACGCGCATCG
BpmI 156 CTCCAG CTGGAG(16/14)
2057 CTGGAG
BpuAI 875 GAAGAC GAAGAC(2/6)
1100 GAAGAC
BsaWI 1324 TCCGGA [AT]^CCGG[AT]
2440 TCCGGA
BseAI 1324 TCCGGA T^CCGGA
2440 TCCGGA
BspEI 1324 TCCGGA T^CCGGA
2440 TCCGGA
DraIII 740 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1898 CACATGGTG
DrdI 1154 GACTCGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1385 GACAAGAGCGTC
EaeI 328 TGGCCA [CT]^GGCC[GA]
552 CGGCCG
FspI 351 TGCGCA TGC^GCA
981 TGCGCA
HgaI 1161 GCGTC GACGC(5/10)
1392 GCGTC
HincII 1412 GTCAAC GT[CT]^[GA]AC
1669 GTCGAC
HindII 1412 GTCAAC GT[CT]^[GA]AC
1669 GTCGAC
KspI 662 CCGCGG CCGC^GG
1575 CCGCGG
MroI 1324 TCCGGA T^CCGGA
2440 TCCGGA
PflMI 865 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2519 CCACTGGTTGG
SacII 662 CCGCGG CCGC^GG
1575 CCGCGG
SmlI 299 CTCGAG C^T[CT][GA]AG
2491 CTTAAG
Tsp509I 290 AATT ^AATT
2333 AATT
Van91I 865 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2519 CCACTGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
Alw44I AlwI AlwNI Aor51HI ApaLI
AspHI AvaI AvaII BanI BbvI
BcnI BfaI BmyI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EarI
Eco47III EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I XhoII