################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AclI AgeI ApaI AscI AseI AsnI Asp700I Asp718I AvrII BbeI BciVI BclI BglII BlnI BlpI Bpu1102I BsaBI BsiWI BsmBI BspDI BspHI BsrBI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI EcoNI EcoO65I EcoRI EcoRV EcoT14I FbaI FseI HpaI KasI KpnI MamI MfeI MunI NarI NcoI NdeI NheI NotI NruI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI StyI SwaI XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccI 1669 GTCGAC GT^[AC][GT]AC AcsI 2332 GAATTT [GA]^AATT[CT] AcyI 1160 GGCGTC G[GA]^CG[CT]C AflII 2491 CTTAAG C^TTAAG AhdI 704 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1486 CCTGAGG CC^T[ACGT]AGG ApoI 2332 GAATTT [GA]^AATT[CT] AspEI 704 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1648 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 328 TGGCCA TGG^CCA BbrPI 2066 CACGTG CAC^GTG BbvCI 1277 GCTGAGG CCTCAGC(-5/-2) BfrI 2491 CTTAAG C^TTAAG BglI 2133 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 2066 CACGTG [CT]AC^GT[GA] BsaHI 1160 GGCGTC G[GA]^CG[CT]C BsiEI 552 CGGCCG CG[GA][CT]^CG BsmI 258 GCATTC GAATGC(1/-1) Bsp1407I 501 TGTACA T^GTACA BspLU11I 1815 ACATGT A^CATGT BsrFI 398 GCCGGC [GA]^CCGG[CT] BsrGI 501 TGTACA T^GTACA BssHII 373 GCGCGC G^CGCGC BssSI 836 CACGAG CACGAG(-5/-1) Bsu36I 1486 CCTGAGG CC^T[ACGT]AGG Cfr10I 398 GCCGGC [GA]^CCGG[CT] DraI 2579 TTTAAA TTT^AAA DraII 2093 GGGACCC [GA]G^G[ACGT]CC[CT] EagI 552 CGGCCG C^GGCCG Eam1105I 704 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 552 CGGCCG C^GGCCG Eco52I 552 CGGCCG C^GGCCG Eco81I 1486 CCTGAGG CC^T[ACGT]AGG EcoO109I 2093 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT22I 2234 ATGCAT ATGCA^T Hin1I 1160 GGCGTC G[GA]^CG[CT]C HindIII 1147 AAGCTT A^AGCTT MluI 771 ACGCGT A^CGCGT MluNI 328 TGGCCA TGG^CCA MscI 328 TGGCCA TGG^CCA NaeI 398 GCCGGC GCC^GGC NgoMIV 398 GCCGGC G^CCGGC NsiI 2234 ATGCAT ATGCA^T PaeR7I 299 CTCGAG C^TCGAG PflFI 1648 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2066 CACGTG CAC^GTG PmlI 2066 CACGTG CAC^GTG PpuMI 2093 GGGACCC [GA]G^G[AT]CC[CT] SacI 1966 GAGCTC GAGCT^C SalI 1669 GTCGAC G^TCGAC SapI 1963 GAAGAGC GCTCTTC(1/4) SbfI 2102 CCTGCAGG CCTGCA^GG SmaI 631 CCCGGG CCC^GGG SphI 366 GCATGC GCATG^C Sse8387I 2102 CCTGCAGG CCTGCA^GG SspBI 501 TGTACA T^GTACA StuI 2495 AGGCCT AGG^CCT TfiI 1082 GAATC G^A[AT]TC Tth111I 1648 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 299 CTCGAG C^TCGAG XmaCI 631 CCCGGG C^CCGGG XmaI 631 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccIII 1324 TCCGGA T^CCGGA 2440 TCCGGA AviII 351 TGCGCA TGC^GCA 981 TGCGCA BamHI 104 GGATCC G^GATCC 636 GGATCC BanII 1133 GAGCCC G[GA]GC[CT]^C 1966 GAGCTC BbsI 875 GAAGAC GAAGAC(2/6) 1100 GAAGAC BcgI 1255 CGAGCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 594 GCACGCGCATCG BpmI 156 CTCCAG CTGGAG(16/14) 2057 CTGGAG BpuAI 875 GAAGAC GAAGAC(2/6) 1100 GAAGAC BsaWI 1324 TCCGGA [AT]^CCGG[AT] 2440 TCCGGA BseAI 1324 TCCGGA T^CCGGA 2440 TCCGGA BspEI 1324 TCCGGA T^CCGGA 2440 TCCGGA DraIII 740 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG 1898 CACATGGTG DrdI 1154 GACTCGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1385 GACAAGAGCGTC EaeI 328 TGGCCA [CT]^GGCC[GA] 552 CGGCCG FspI 351 TGCGCA TGC^GCA 981 TGCGCA HgaI 1161 GCGTC GACGC(5/10) 1392 GCGTC HincII 1412 GTCAAC GT[CT]^[GA]AC 1669 GTCGAC HindII 1412 GTCAAC GT[CT]^[GA]AC 1669 GTCGAC KspI 662 CCGCGG CCGC^GG 1575 CCGCGG MroI 1324 TCCGGA T^CCGGA 2440 TCCGGA PflMI 865 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2519 CCACTGGTTGG SacII 662 CCGCGG CCGC^GG 1575 CCGCGG SmlI 299 CTCGAG C^T[CT][GA]AG 2491 CTTAAG Tsp509I 290 AATT ^AATT 2333 AATT Van91I 865 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2519 CCACTGGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccII AciI AfaI AflIII AluI Alw44I AlwI AlwNI Aor51HI ApaLI AspHI AvaI AvaII BanI BbvI BcnI BfaI BmyI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI Eco47III EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp45I TspRI TthHB8I XhoII