##################################
# NO CUT ENZYMES ( 36 enzymes) #
##################################
AccIII AclI AflII AgeI AseI
AsnI AvrII BciVI BfrI BlnI
BseAI BspEI Bst1107I BstZ17I EcoRV
HindIII MfeI MluI MroI MunI
NotI NruI PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI SalI
SnaBI SpeI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 6143 GGTACC G^GTACC
AccI 1034 GTCTAC GT^[AC][GT]AC
AhdI 968 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2323 GTGCAC G^TGCAC
ApaLI 2323 GTGCAC G^TGCAC
AscI 176 GGCGCGCC GG^CGCGCC
Asp718I 6143 GGTACC G^GTACC
AspEI 968 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2109 GGATCC G^GATCC
BbrPI 2318 CACGTG CAC^GTG
BsaBI 3202 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 1149 CGTACG C^GTACG
BspHI 4446 TCATGA T^CATGA
BstBI 2945 TTCGAA TT^CGAA
CpoI 6682 CGGACCG CG^G[AT]CCG
Eam1105I 968 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2572 GAATTC G^AATTC
HpaI 7236 GTTAAC GTT^AAC
KpnI 6143 GGTACC GGTAC^C
MamI 3202 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 3570 CCATGG C^CATGG
NdeI 4245 CATATG CA^TATG
NheI 7471 GCTAGC G^CTAGC
NspV 2945 TTCGAA TT^CGAA
PmaCI 2318 CACGTG CAC^GTG
PmlI 2318 CACGTG CAC^GTG
PshAI 2951 GACGTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4446 TCATGA T^CATGA
RsrII 6682 CGGACCG CG^G[AT]CCG
SfiI 83 GGCCTGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2945 TTCGAA TT^CGAA
SgrAI 1084 CGCCGGCG C[GA]^CCGG[CT]G
StuI 2022 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AatII 4982 GACGTC GACGT^C
5080 GACGTC
BsaAI 2318 CACGTG [CT]AC^GT[GA]
2414 TACGTG
BsmBI 2882 GAGACG CGTCTC(1/5)
3875 GAGACG
BspDI 839 ATCGAT AT^CGAT
1472 ATCGAT
BssHII 177 GCGCGC G^CGCGC
4958 GCGCGC
BstEII 4849 GGTCACC G^GT[ACGT]ACC
5270 GGTGACC
BstPI 4849 GGTCACC G^GT[ACGT]ACC
5270 GGTGACC
ClaI 839 ATCGAT AT^CGAT
1472 ATCGAT
DraI 7253 TTTAAA TTT^AAA
7497 TTTAAA
DrdI 4976 GACGAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5044 GACAGACGGGTC
EcoO65I 4849 GGTCACC G^GT[ACGT]ACC
5270 GGTGACC
FseI 1673 GGCCGGCC GGCCGG^CC
3260 GGCCGGCC
SacI 6479 GAGCTC GAGCT^C
6831 GAGCTC
ScaI 596 AGTACT AGT^ACT
855 AGTACT
SexAI 5037 ACCAGGT A^CC[AT]GGT
5232 ACCTGGT
XcmI 816 CCAAGCTCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3738 CCACTGGCCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 173 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApaI ApoI Asp700I AspHI
AspI AvaI AvaII AviII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcgI BclI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NsiI NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacII
SapI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SfoI SmaI SmlI
SphI Sse8387I SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII
XmaCI XmaI XmnI