##################################
# NO CUT ENZYMES ( 28 enzymes) #
##################################
AgeI AhdI AscI AspEI BcgI
BsiWI BspDI ClaI CpoI EagI
Eam1105I EclXI Eco52I FseI HpaI
MluI NotI PacI PinAI PmeI
PvuI RsrII SalI SbfI SgrAI
SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 2289 GACGTC GACGT^C
Acc65I 2849 GGTACC G^GTACC
AclI 2883 AACGTT AA^CGTT
AocI 6957 CCTAAGG CC^T[ACGT]AGG
Asp718I 2849 GGTACC G^GTACC
AviII 108 TGCGCA TGC^GCA
AvrII 6827 CCTAGG C^CTAGG
BamHI 6660 GGATCC G^GATCC
BbeI 2031 GGCGCC GGCGC^C
BciVI 8049 GGATAC GTATCC(6/5)
BlnI 6827 CCTAGG C^CTAGG
BlpI 3816 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3816 GCTGAGC GC^T[ACGT]AGC
BsaBI 3327 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1221 GAGACG CGTCTC(1/5)
BspLU11I 6781 ACATGT A^CATGT
BstAPI 2939 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2211 TTCGAA TT^CGAA
Bsu36I 6957 CCTAAGG CC^T[ACGT]AGG
CelII 3816 GCTGAGC GC^T[ACGT]AGC
DraIII 834 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2121 GACATGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 6957 CCTAAGG CC^T[ACGT]AGG
EcoNI 11 CCTCGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3918 GATATC GAT^ATC
FspI 108 TGCGCA TGC^GCA
HindIII 1103 AAGCTT A^AGCTT
KasI 2031 GGCGCC G^GCGCC
KpnI 2849 GGTACC GGTAC^C
MamI 3327 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6553 CAATTG C^AATTG
MunI 6553 CAATTG C^AATTG
NaeI 546 GCCGGC GCC^GGC
NarI 2031 GGCGCC GG^CGCC
NdeI 7094 CATATG CA^TATG
NgoMIV 546 GCCGGC G^CCGGC
NheI 4500 GCTAGC G^CTAGC
NspV 2211 TTCGAA TT^CGAA
PpuMI 985 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2883 AACGTT AA^CGTT
SapI 2896 GCTCTTC GCTCTTC(1/4)
SfoI 2031 GGCGCC GGC^GCC
SfuI 2211 TTCGAA TT^CGAA
SnaBI 5110 TACGTA TAC^GTA
SphI 111 GCATGC GCATG^C
XbaI 5526 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 230 TCCGGA T^CCGGA
2755 TCCGGA
Alw44I 120 GTGCAC G^TGCAC
155 GTGCAC
Aor51HI 89 AGCGCT AGC^GCT
1080 AGCGCT
ApaLI 120 GTGCAC G^TGCAC
155 GTGCAC
Asp700I 3342 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5723 GAATATTTTC
BbrPI 1554 CACGTG CAC^GTG
6289 CACGTG
BbsI 2403 GAAGAC GAAGAC(2/6)
2130 GTCTTC
BclI 3326 TGATCA T^GATCA
3332 TGATCA
BpuAI 2403 GAAGAC GAAGAC(2/6)
2130 GTCTTC
BsaI 577 GGTCTC GGTCTC(1/5)
2073 GGTCTC
BsaWI 230 TCCGGA [AT]^CCGG[AT]
2755 TCCGGA
BseAI 230 TCCGGA T^CCGGA
2755 TCCGGA
BsiEI 1472 CGACCG CG[GA][CT]^CG
2456 CGACCG
Bsp1407I 7267 TGTACA T^GTACA
8324 TGTACA
BspEI 230 TCCGGA T^CCGGA
2755 TCCGGA
BsrBI 200 CCGCTC CCGCTC(-3/-3)
2963 CCGCTC
BsrGI 7267 TGTACA T^GTACA
8324 TGTACA
BssSI 2193 CACGAG CACGAG(-5/-1)
1194 CTCGTG
Bst1107I 6932 GTATAC GTA^TAC
7441 GTATAC
BstZ17I 6932 GTATAC GTA^TAC
7441 GTATAC
Eco47III 89 AGCGCT AGC^GCT
1080 AGCGCT
FbaI 3326 TGATCA T^GATCA
3332 TGATCA
MroI 230 TCCGGA T^CCGGA
2755 TCCGGA
NcoI 625 CCATGG C^CATGG
3307 CCATGG
NruI 1751 TCGCGA TCG^CGA
2057 TCGCGA
PmaCI 1554 CACGTG CAC^GTG
6289 CACGTG
PmlI 1554 CACGTG CAC^GTG
6289 CACGTG
PshAI 2067 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2387 GACTGTTGTC
PvuII 7907 CAGCTG CAG^CTG
8033 CAGCTG
SfiI 20 GGCCAGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
506 GGCCTCCGGGGCC
SspBI 7267 TGTACA T^GTACA
8324 TGTACA
XmnI 3342 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5723 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI ApaI ApoI AseI AsnI
AspHI AspI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BfrI BglI BglII BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrI BssHII
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PaeR7I PflFI PflMI PleI PshBI
PstI RcaI RsaI SacI SacII
Sau3AI Sau96I ScaI ScrFI SexAI
SfaNI SfcI SmaI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoI XhoII
XmaCI XmaI