################################## # NO CUT ENZYMES ( 28 enzymes) # ################################## AgeI AhdI AscI AspEI BcgI BsiWI BspDI ClaI CpoI EagI Eam1105I EclXI Eco52I FseI HpaI MluI NotI PacI PinAI PmeI PvuI RsrII SalI SbfI SgrAI SpeI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 2289 GACGTC GACGT^C Acc65I 2849 GGTACC G^GTACC AclI 2883 AACGTT AA^CGTT AocI 6957 CCTAAGG CC^T[ACGT]AGG Asp718I 2849 GGTACC G^GTACC AviII 108 TGCGCA TGC^GCA AvrII 6827 CCTAGG C^CTAGG BamHI 6660 GGATCC G^GATCC BbeI 2031 GGCGCC GGCGC^C BciVI 8049 GGATAC GTATCC(6/5) BlnI 6827 CCTAGG C^CTAGG BlpI 3816 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3816 GCTGAGC GC^T[ACGT]AGC BsaBI 3327 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1221 GAGACG CGTCTC(1/5) BspLU11I 6781 ACATGT A^CATGT BstAPI 2939 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2211 TTCGAA TT^CGAA Bsu36I 6957 CCTAAGG CC^T[ACGT]AGG CelII 3816 GCTGAGC GC^T[ACGT]AGC DraIII 834 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2121 GACATGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 6957 CCTAAGG CC^T[ACGT]AGG EcoNI 11 CCTCGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 3918 GATATC GAT^ATC FspI 108 TGCGCA TGC^GCA HindIII 1103 AAGCTT A^AGCTT KasI 2031 GGCGCC G^GCGCC KpnI 2849 GGTACC GGTAC^C MamI 3327 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 6553 CAATTG C^AATTG MunI 6553 CAATTG C^AATTG NaeI 546 GCCGGC GCC^GGC NarI 2031 GGCGCC GG^CGCC NdeI 7094 CATATG CA^TATG NgoMIV 546 GCCGGC G^CCGGC NheI 4500 GCTAGC G^CTAGC NspV 2211 TTCGAA TT^CGAA PpuMI 985 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 2883 AACGTT AA^CGTT SapI 2896 GCTCTTC GCTCTTC(1/4) SfoI 2031 GGCGCC GGC^GCC SfuI 2211 TTCGAA TT^CGAA SnaBI 5110 TACGTA TAC^GTA SphI 111 GCATGC GCATG^C XbaI 5526 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccIII 230 TCCGGA T^CCGGA 2755 TCCGGA Alw44I 120 GTGCAC G^TGCAC 155 GTGCAC Aor51HI 89 AGCGCT AGC^GCT 1080 AGCGCT ApaLI 120 GTGCAC G^TGCAC 155 GTGCAC Asp700I 3342 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5723 GAATATTTTC BbrPI 1554 CACGTG CAC^GTG 6289 CACGTG BbsI 2403 GAAGAC GAAGAC(2/6) 2130 GTCTTC BclI 3326 TGATCA T^GATCA 3332 TGATCA BpuAI 2403 GAAGAC GAAGAC(2/6) 2130 GTCTTC BsaI 577 GGTCTC GGTCTC(1/5) 2073 GGTCTC BsaWI 230 TCCGGA [AT]^CCGG[AT] 2755 TCCGGA BseAI 230 TCCGGA T^CCGGA 2755 TCCGGA BsiEI 1472 CGACCG CG[GA][CT]^CG 2456 CGACCG Bsp1407I 7267 TGTACA T^GTACA 8324 TGTACA BspEI 230 TCCGGA T^CCGGA 2755 TCCGGA BsrBI 200 CCGCTC CCGCTC(-3/-3) 2963 CCGCTC BsrGI 7267 TGTACA T^GTACA 8324 TGTACA BssSI 2193 CACGAG CACGAG(-5/-1) 1194 CTCGTG Bst1107I 6932 GTATAC GTA^TAC 7441 GTATAC BstZ17I 6932 GTATAC GTA^TAC 7441 GTATAC Eco47III 89 AGCGCT AGC^GCT 1080 AGCGCT FbaI 3326 TGATCA T^GATCA 3332 TGATCA MroI 230 TCCGGA T^CCGGA 2755 TCCGGA NcoI 625 CCATGG C^CATGG 3307 CCATGG NruI 1751 TCGCGA TCG^CGA 2057 TCGCGA PmaCI 1554 CACGTG CAC^GTG 6289 CACGTG PmlI 1554 CACGTG CAC^GTG 6289 CACGTG PshAI 2067 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2387 GACTGTTGTC PvuII 7907 CAGCTG CAG^CTG 8033 CAGCTG SfiI 20 GGCCAGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 506 GGCCTCCGGGGCC SspBI 7267 TGTACA T^GTACA 8324 TGTACA XmnI 3342 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5723 GAATATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 152 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflII AflIII AluI AlwI AlwNI ApaI ApoI AseI AsnI AspHI AspI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BfrI BglI BglII BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrI BssHII BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PaeR7I PflFI PflMI PleI PshBI PstI RcaI RsaI SacI SacII Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmaI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI