##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
BamHI BbeI BcgI BclI BsaBI
BsiWI BsmBI BspDI BsrBI BssHII
BstBI BstEII BstPI ClaI CpoI
DraIII DrdI Eco47III EcoO65I EcoRI
FbaI FseI KasI MamI MluI
NaeI NarI NgoMIV NotI NruI
NspV PaeR7I PinAI Psp1406I PvuI
RsrII SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 4985 GGTACC G^GTACC
AccIII 5575 TCCGGA T^CCGGA
AcyI 1106 GACGCC G[GA]^CG[CT]C
AhdI 1983 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 5162 ATTAAT AT^TAAT
AsnI 5162 ATTAAT AT^TAAT
Asp718I 4985 GGTACC G^GTACC
AspEI 1983 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2335 TGCGCA TGC^GCA
BglII 2597 AGATCT A^GATCT
BsaHI 1106 GACGCC G[GA]^CG[CT]C
BsaWI 5575 TCCGGA [AT]^CCGG[AT]
BseAI 5575 TCCGGA T^CCGGA
BsiEI 6226 CGGCCG CG[GA][CT]^CG
BsmI 1910 GAATGC GAATGC(1/-1)
BspEI 5575 TCCGGA T^CCGGA
EagI 6226 CGGCCG C^GGCCG
Eam1105I 1983 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 6226 CGGCCG C^GGCCG
Eco52I 6226 CGGCCG C^GGCCG
EcoRV 439 GATATC GAT^ATC
EcoT22I 5060 ATGCAT ATGCA^T
FspI 2335 TGCGCA TGC^GCA
Hin1I 1106 GACGCC G[GA]^CG[CT]C
HpaI 5212 GTTAAC GTT^AAC
KpnI 4985 GGTACC GGTAC^C
KspI 2437 CCGCGG CCGC^GG
MfeI 7326 CAATTG C^AATTG
MroI 5575 TCCGGA T^CCGGA
MunI 7326 CAATTG C^AATTG
NheI 514 GCTAGC G^CTAGC
NsiI 5060 ATGCAT ATGCA^T
PacI 5159 TTAATTAA TTAAT^TAA
PmeI 5241 GTTTAAAC GTTT^AAAC
PshBI 5162 ATTAAT AT^TAAT
SacII 2437 CCGCGG CCGC^GG
SalI 2940 GTCGAC G^TCGAC
SpeI 2835 ACTAGT A^CTAGT
SwaI 4523 ATTTAAAT ATTT^AAAT
XbaI 81 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 3302 CTTAAG C^TTAAG
4829 CTTAAG
AvaI 4012 CCCGAG C^[CT]CG[GA]G
6082 CTCGGG
AvrII 38 CCTAGG C^CTAGG
5197 CCTAGG
BciVI 205 GTATCC GTATCC(6/5)
2062 GTATCC
BfrI 3302 CTTAAG C^TTAAG
4829 CTTAAG
BlnI 38 CCTAGG C^CTAGG
5197 CCTAGG
BsaI 2784 GAGACC GGTCTC(1/5)
6305 GAGACC
BsoBI 4012 CCCGAG C^[CT]CG[GA]G
6082 CTCGGG
BspHI 1890 TCATGA T^CATGA
3917 TCATGA
BspLU11I 3687 ACATGT A^CATGT
5390 ACATGT
BsrDI 5147 CATTGC GCAATG(2/0)
6166 CATTGC
BsrFI 5556 ACCGGC [GA]^CCGG[CT]
6954 ACCGGC
Cfr10I 5556 ACCGGC [GA]^CCGG[CT]
6954 ACCGGC
EcoNI 3952 CCTGACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5585 CCTGCTATAGG
HaeII 2181 GGCGCT [GA]GCGC^[CT]
4049 AGCGCC
PshAI 662 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4972 GACTAAGGTC
RcaI 1890 TCATGA T^CATGA
3917 TCATGA
ScaI 2544 AGTACT AGT^ACT
5141 AGTACT
SexAI 1875 ACCTGGT A^CC[AT]GGT
3977 ACCTGGT
SphI 2323 GCATGC GCATG^C
3722 GCATGC
SspI 2143 AATATT AAT^ATT
3511 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI Asp700I
AspHI AspI AvaII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BssSI Bst1107I
BstAPI BstF5I BstNI BstUI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI