##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AclI AcsI AflII AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI BfrI BsiWI BsmI
BspDI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
HindIII HpaI MfeI MluI MunI
NdeI NheI NruI NspV PacI
PmeI PshBI Psp1406I PvuI RsrII
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AvrII 706 CCTAGG C^CTAGG
BclI 6903 TGATCA T^GATCA
BfaI 707 CTAG C^TAG
BlnI 706 CCTAGG C^CTAGG
BsaBI 145 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 1416 TCATGA T^CATGA
BspLU11I 2544 ACATGT A^CATGT
DraI 8042 TTTAAA TTT^AAA
EagI 2011 CGGCCG C^GGCCG
EclXI 2011 CGGCCG C^GGCCG
Eco52I 2011 CGGCCG C^GGCCG
EcoT22I 3960 ATGCAT ATGCA^T
FbaI 6903 TGATCA T^GATCA
FseI 1288 GGCCGGCC GGCCGG^CC
MaeI 707 CTAG C^TAG
MamI 145 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 2010 GCGGCCGC GC^GGCCGC
NsiI 3960 ATGCAT ATGCA^T
PaeR7I 419 CTCGAG C^TCGAG
RcaI 1416 TCATGA T^CATGA
SalI 1463 GTCGAC G^TCGAC
SfiI 2488 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 662 GATTC G^A[AT]TC
XhoI 419 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 3705 GGTACC G^GTACC
6666 GGTACC
AccIII 726 TCCGGA T^CCGGA
918 TCCGGA
AgeI 5136 ACCGGT A^CCGGT
6882 ACCGGT
Asp718I 3705 GGTACC G^GTACC
6666 GGTACC
AviII 4990 TGCGCA TGC^GCA
5229 TGCGCA
BbeI 235 GGCGCC GGCGC^C
997 GGCGCC
BcgI 1489 CGAGACTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5427 GCACGGATGTCG
BciVI 1732 GGATAC GTATCC(6/5)
6258 GTATCC
BglII 144 AGATCT A^GATCT
4440 AGATCT
BseAI 726 TCCGGA T^CCGGA
918 TCCGGA
Bsp1407I 6510 TGTACA T^GTACA
8018 TGTACA
BspEI 726 TCCGGA T^CCGGA
918 TCCGGA
BsrDI 3631 CATTGC GCAATG(2/0)
3963 CATTGC
BsrGI 6510 TGTACA T^GTACA
8018 TGTACA
DrdI 2765 GACGCCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7094 GACTACTCGGTC
FspI 4990 TGCGCA TGC^GCA
5229 TGCGCA
KasI 235 GGCGCC G^GCGCC
997 GGCGCC
KpnI 3705 GGTACC GGTAC^C
6666 GGTACC
MroI 726 TCCGGA T^CCGGA
918 TCCGGA
MseI 7615 TTAA T^TAA
8043 TTAA
NarI 235 GGCGCC GG^CGCC
997 GGCGCC
PinAI 5136 ACCGGT A^CCGGT
6882 ACCGGT
PshAI 6226 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7442 GACAGAAGTC
ScaI 2649 AGTACT AGT^ACT
5964 AGTACT
SexAI 3111 ACCAGGT A^CC[AT]GGT
5601 ACCTGGT
SfoI 235 GGCGCC GGC^GCC
997 GGCGCC
SphI 5274 GCATGC GCATG^C
6117 GCATGC
SspBI 6510 TGTACA T^GTACA
8018 TGTACA
Tru9I 7615 TTAA T^TAA
8043 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaI ApaLI AspHI AspI
AvaI AvaII BalI BamHI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmaI SmlI Sse8387I StuI StyI
TaqI TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI