##################################
# NO CUT ENZYMES ( 31 enzymes) #
##################################
AatII AclI AgeI AocI AscI
AviII BciVI BsiWI BspDI Bsu36I
ClaI CpoI Eco81I EcoRV FseI
FspI HpaI MfeI MluI MunI
NotI NruI PacI PinAI PshAI
Psp1406I RsrII SnaBI SphI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 3565 GGTACC G^GTACC
AccIII 1596 TCCGGA T^CCGGA
Aor51HI 316 AGCGCT AGC^GCT
AseI 5957 ATTAAT AT^TAAT
AsnI 5957 ATTAAT AT^TAAT
Asp718I 3565 GGTACC G^GTACC
AvrII 5796 CCTAGG C^CTAGG
BcgI 5023 CGATCGGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 7465 TGATCA T^GATCA
BglII 4335 AGATCT A^GATCT
BlnI 5796 CCTAGG C^CTAGG
BlpI 4008 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4008 GCTCAGC GC^T[ACGT]AGC
BseAI 1596 TCCGGA T^CCGGA
BspEI 1596 TCCGGA T^CCGGA
BspLU11I 3280 ACATGT A^CATGT
Bst1107I 7661 GTATAC GTA^TAC
BstBI 671 TTCGAA TT^CGAA
BstZ17I 7661 GTATAC GTA^TAC
CelII 4008 GCTCAGC GC^T[ACGT]AGC
DraIII 6340 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 316 AGCGCT AGC^GCT
EcoNI 5637 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 7465 TGATCA T^GATCA
HindIII 3171 AAGCTT A^AGCTT
KpnI 3565 GGTACC GGTAC^C
MroI 1596 TCCGGA T^CCGGA
NspV 671 TTCGAA TT^CGAA
PflMI 746 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 5972 GTTTAAAC GTTT^AAAC
PshBI 5957 ATTAAT AT^TAAT
SalI 1367 GTCGAC G^TCGAC
SexAI 2140 ACCTGGT A^CC[AT]GGT
SfiI 6648 GGCCTCCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 671 TTCGAA TT^CGAA
SpeI 5843 ACTAGT A^CTAGT
SspI 5165 AATATT AAT^ATT
Van91I 746 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 4696 CTTAAG C^TTAAG
7826 CTTAAG
AspI 2096 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7016 GACCACGTC
BbsI 6939 GAAGAC GAAGAC(2/6)
3048 GTCTTC
BbvCI 108 GCTGAGG CCTCAGC(-5/-2)
2516 GCTGAGG
BfrI 4696 CTTAAG C^TTAAG
7826 CTTAAG
BpuAI 6939 GAAGAC GAAGAC(2/6)
3048 GTCTTC
BsaBI 965 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3203 GATCCTCATC
BsaWI 1267 ACCGGA [AT]^CCGG[AT]
1596 TCCGGA
Bsp1407I 3277 TGTACA T^GTACA
5621 TGTACA
BspHI 539 TCATGA T^CATGA
7729 TCATGA
BsrGI 3277 TGTACA T^GTACA
5621 TGTACA
BstEII 528 GGTTACC G^GT[ACGT]ACC
3869 GGTCACC
BstPI 528 GGTTACC G^GT[ACGT]ACC
3869 GGTCACC
EcoO65I 528 GGTTACC G^GT[ACGT]ACC
3869 GGTCACC
MamI 965 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3203 GATCCTCATC
NdeI 2984 CATATG CA^TATG
5439 CATATG
NheI 698 GCTAGC G^CTAGC
6632 GCTAGC
PaeR7I 3618 CTCGAG C^TCGAG
4130 CTCGAG
PflFI 2096 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7016 GACCACGTC
PvuI 3454 CGATCG CGAT^CG
5023 CGATCG
RcaI 539 TCATGA T^CATGA
7729 TCATGA
ScaI 3421 AGTACT AGT^ACT
3781 AGTACT
SmaI 2587 CCCGGG CCC^GGG
4517 CCCGGG
SspBI 3277 TGTACA T^GTACA
5621 TGTACA
Tth111I 2096 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7016 GACCACGTC
XhoI 3618 CTCGAG C^TCGAG
4130 CTCGAG
XmaCI 2587 CCCGGG C^CCGGG
4517 CCCGGG
XmaI 2587 CCCGGG C^CCGGG
4517 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 161 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI Alw44I
AlwI AlwNI ApaI ApaLI ApoI
Asp700I AspEI AspHI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbrPI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco52I EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NsiI NspI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SacII SapI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SfoI
SgrAI SmlI Sse8387I StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI