##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AatII AclI Alw44I ApaLI AscI
AseI AsnI AviII BglI BsaI
BsiWI BsmI BsrDI BssHII BstBI
EagI EclXI Eco52I EcoT22I FspI
KspI MfeI MunI NheI NotI
NruI NsiI NspV PacI PmeI
PshBI Psp1406I SacII SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 5048 GGTACC G^GTACC
AflII 3223 CTTAAG C^TTAAG
AgeI 3695 ACCGGT A^CCGGT
AhdI 4222 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 5048 GGTACC G^GTACC
AspEI 4222 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 6816 CCTAGG C^CTAGG
BamHI 6156 GGATCC G^GATCC
BbeI 5989 GGCGCC GGCGC^C
BcgI 1645 CGATGCACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 7019 GGATAC GTATCC(6/5)
BfrI 3223 CTTAAG C^TTAAG
BglII 5363 AGATCT A^GATCT
BlnI 6816 CCTAGG C^CTAGG
BsiEI 3507 CGATCG CG[GA][CT]^CG
Bsp1407I 7297 TGTACA T^GTACA
BspLU11I 2360 ACATGT A^CATGT
BsrGI 7297 TGTACA T^GTACA
Bst1107I 6441 GTATAC GTA^TAC
BstAPI 3018 GCATGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1305 CCAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 6441 GTATAC GTA^TAC
CpoI 453 CGGACCG CG^G[AT]CCG
DraI 5476 TTTAAA TTT^AAA
DrdI 3705 GACTCGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4222 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 907 GATATC GAT^ATC
HpaI 4189 GTTAAC GTT^AAC
KasI 5989 GGCGCC G^GCGCC
KpnI 5048 GGTACC GGTAC^C
MluI 1565 ACGCGT A^CGCGT
NarI 5989 GGCGCC GG^CGCC
NdeI 2120 CATATG CA^TATG
PinAI 3695 ACCGGT A^CCGGT
PshAI 1090 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3507 CGATCG CGAT^CG
RsrII 453 CGGACCG CG^G[AT]CCG
SalI 5082 GTCGAC G^TCGAC
SbfI 6010 CCTGCAGG CCTGCA^GG
SfoI 5989 GGCGCC GGC^GCC
Sse8387I 6010 CCTGCAGG CCTGCA^GG
SspBI 7297 TGTACA T^GTACA
SspI 127 AATATT AAT^ATT
XbaI 4998 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 871 TCCGGA T^CCGGA
4313 TCCGGA
AcyI 5085 GACGCC G[GA]^CG[CT]C
5989 GGCGCC
AflIII 1565 ACGCGT A^C[GA][CT]GT
2360 ACATGT
Aor51HI 4727 AGCGCT AGC^GCT
6212 AGCGCT
BbrPI 2133 CACGTG CAC^GTG
6689 CACGTG
BsaAI 2133 CACGTG [CT]AC^GT[GA]
6689 CACGTG
BsaBI 717 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1741 GATGGAAATC
BsaHI 5085 GACGCC G[GA]^CG[CT]C
5989 GGCGCC
BseAI 871 TCCGGA T^CCGGA
4313 TCCGGA
BsmBI 4760 CGTCTC CGTCTC(1/5)
1273 GAGACG
BspDI 3466 ATCGAT AT^CGAT
3505 ATCGAT
BspEI 871 TCCGGA T^CCGGA
4313 TCCGGA
BspHI 665 TCATGA T^CATGA
3212 TCATGA
BsrBI 2551 GAGCGG CCGCTC(-3/-3)
4972 GAGCGG
BssSI 3176 CACGAG CACGAG(-5/-1)
1343 CTCGTG
BstEII 5188 GGTGACC G^GT[ACGT]ACC
6911 GGTAACC
BstPI 5188 GGTGACC G^GT[ACGT]ACC
6911 GGTAACC
ClaI 3466 ATCGAT AT^CGAT
3505 ATCGAT
Eco47III 4727 AGCGCT AGC^GCT
6212 AGCGCT
EcoO65I 5188 GGTGACC G^GT[ACGT]ACC
6911 GGTAACC
EcoRI 2638 GAATTC G^AATTC
3171 GAATTC
FseI 1156 GGCCGGCC GGCCGG^CC
1198 GGCCGGCC
Hin1I 5085 GACGCC G[GA]^CG[CT]C
5989 GGCGCC
HindIII 1009 AAGCTT A^AGCTT
2678 AAGCTT
MamI 717 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1741 GATGGAAATC
MroI 871 TCCGGA T^CCGGA
4313 TCCGGA
NcoI 5412 CCATGG C^CATGG
5627 CCATGG
PaeR7I 1083 CTCGAG C^TCGAG
5213 CTCGAG
PmaCI 2133 CACGTG CAC^GTG
6689 CACGTG
PmlI 2133 CACGTG CAC^GTG
6689 CACGTG
RcaI 665 TCATGA T^CATGA
3212 TCATGA
SexAI 3191 ACCAGGT A^CC[AT]GGT
3767 ACCTGGT
XhoI 1083 CTCGAG C^TCGAG
5213 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmnI