##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AclI AflII AflIII AhdI
Alw44I ApaLI AspEI AvrII BbrPI
BfrI BglII BlnI BsaAI BsaBI
BsiWI BsmBI BspDI BspLU11I BstEII
BstPI ClaI CpoI Eam1105I EcoO65I
HpaI MamI MluI NotI NspI
PacI PaeR7I PmaCI PmeI PmlI
Psp1406I PvuI RsrII SalI SbfI
ScaI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AgeI 5549 ACCGGT A^CCGGT
AscI 47 GGCGCGCC GG^CGCGCC
AseI 7302 ATTAAT AT^TAAT
AsnI 7302 ATTAAT AT^TAAT
BclI 7139 TGATCA T^GATCA
BssHII 48 GCGCGC G^CGCGC
Bst1107I 5609 GTATAC GTA^TAC
BstBI 5651 TTCGAA TT^CGAA
BstZ17I 5609 GTATAC GTA^TAC
DraIII 1220 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3193 CGGCCG C^GGCCG
EclXI 3193 CGGCCG C^GGCCG
Eco52I 3193 CGGCCG C^GGCCG
EcoRV 4683 GATATC GAT^ATC
EcoT22I 6270 ATGCAT ATGCA^T
FbaI 7139 TGATCA T^GATCA
FseI 4089 GGCCGGCC GGCCGG^CC
HincII 5318 GTCAAC GT[CT]^[GA]AC
HindII 5318 GTCAAC GT[CT]^[GA]AC
HindIII 6183 AAGCTT A^AGCTT
NaeI 4090 GCCGGC GCC^GGC
NgoMIV 4090 GCCGGC G^CCGGC
NheI 4403 GCTAGC G^CTAGC
NruI 21 TCGCGA TCG^CGA
NsiI 6270 ATGCAT ATGCA^T
NspV 5651 TTCGAA TT^CGAA
PinAI 5549 ACCGGT A^CCGGT
PshAI 4787 GACTCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 7302 ATTAAT AT^TAAT
SfuI 5651 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 2119 GGTACC G^GTACC
6232 GGTACC
AccI 2420 GTCTAC GT^[AC][GT]AC
5609 GTATAC
AccIII 2640 TCCGGA T^CCGGA
3510 TCCGGA
AcyI 179 GGCGCC G[GA]^CG[CT]C
1923 GGCGCC
Asp718I 2119 GGTACC G^GTACC
6232 GGTACC
AspI 4285 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5859 GACAGAGTC
AviII 1515 TGCGCA TGC^GCA
3715 TGCGCA
BamHI 4047 GGATCC G^GATCC
4727 GGATCC
BbeI 179 GGCGCC GGCGC^C
1923 GGCGCC
BsaHI 179 GGCGCC G[GA]^CG[CT]C
1923 GGCGCC
BseAI 2640 TCCGGA T^CCGGA
3510 TCCGGA
BsiEI 235 CGACCG CG[GA][CT]^CG
3193 CGGCCG
BspEI 2640 TCCGGA T^CCGGA
3510 TCCGGA
BspHI 328 TCATGA T^CATGA
552 TCATGA
BsrDI 3085 CATTGC GCAATG(2/0)
3198 GCAATG
FspI 1515 TGCGCA TGC^GCA
3715 TGCGCA
HgaI 92 GCGTC GACGC(5/10)
424 GCGTC
Hin1I 179 GGCGCC G[GA]^CG[CT]C
1923 GGCGCC
KasI 179 GGCGCC G^GCGCC
1923 GGCGCC
KpnI 2119 GGTACC GGTAC^C
6232 GGTACC
MfeI 6324 CAATTG C^AATTG
6936 CAATTG
MroI 2640 TCCGGA T^CCGGA
3510 TCCGGA
MunI 6324 CAATTG C^AATTG
6936 CAATTG
NarI 179 GGCGCC GG^CGCC
1923 GGCGCC
NcoI 1833 CCATGG C^CATGG
2816 CCATGG
NdeI 3719 CATATG CA^TATG
5792 CATATG
PflFI 4285 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5859 GACAGAGTC
RcaI 328 TCATGA T^CATGA
552 TCATGA
SacI 5578 GAGCTC GAGCT^C
6445 GAGCTC
SfoI 179 GGCGCC GGC^GCC
1923 GGCGCC
SspI 6967 AATATT AAT^ATT
7226 AATATT
Tth111I 4285 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5859 GACAGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcgI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacII
SapI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmaI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI