##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AclI Aor51HI AscI AseI AsnI
AvrII BalI BbrPI BcgI BlnI
BsaAI BsaBI BsiEI BsiWI BspDI
BssHII BstEII BstPI ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoT22I FseI KspI MamI
MluI MluNI MscI NaeI NdeI
NgoMIV NotI NruI NsiI PacI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
SexAI SgrAI SnaBI Sse8387I StuI
SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 4482 GACGTC GACGT^C
Acc65I 1037 GGTACC G^GTACC
AccIII 267 TCCGGA T^CCGGA
AflII 2710 CTTAAG C^TTAAG
AgeI 4158 ACCGGT A^CCGGT
AhdI 4883 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3320 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1037 GGTACC G^GTACC
AspEI 4883 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 366 TGCGCA TGC^GCA
BbeI 1732 GGCGCC GGCGC^C
BfrI 2710 CTTAAG C^TTAAG
BseAI 267 TCCGGA T^CCGGA
BsmBI 4484 CGTCTC CGTCTC(1/5)
BspEI 267 TCCGGA T^CCGGA
BsrBI 428 GAGCGG CCGCTC(-3/-3)
BsrFI 4158 ACCGGT [GA]^CCGG[CT]
BstAPI 6941 GCACTAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 4158 ACCGGT [GA]^CCGG[CT]
Eam1105I 4883 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2186 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3616 GAATTC G^AATTC
FspI 366 TGCGCA TGC^GCA
HgaI 7012 GACGC GACGC(5/10)
HpaI 6686 GTTAAC GTT^AAC
KasI 1732 GGCGCC G^GCGCC
KpnI 1037 GGTACC GGTAC^C
MroI 267 TCCGGA T^CCGGA
NarI 1732 GGCGCC GG^CGCC
NheI 4553 GCTAGC G^CTAGC
PflMI 954 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 4158 ACCGGT A^CCGGT
SalI 942 GTCGAC G^TCGAC
SfiI 2189 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1732 GGCGCC GGC^GCC
SphI 7368 GCATGC GCATG^C
Van91I 954 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3320 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 1732 GGCGCC G[GA]^CG[CT]C
4482 GACGTC
ApaI 3173 GGGCCC GGGCC^C
5869 GGGCCC
BamHI 2447 GGATCC G^GATCC
4427 GGATCC
BsaHI 1732 GGCGCC G[GA]^CG[CT]C
4482 GACGTC
BspHI 2937 TCATGA T^CATGA
6377 TCATGA
BspMI 6459 ACCTGC ACCTGC(4/8)
2504 GCAGGT
Bst1107I 1078 GTATAC GTA^TAC
7994 GTATAC
BstBI 2481 TTCGAA TT^CGAA
5184 TTCGAA
BstZ17I 1078 GTATAC GTA^TAC
7994 GTATAC
DraIII 1349 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
6204 CACACGGTG
Hin1I 1732 GGCGCC G[GA]^CG[CT]C
4482 GACGTC
NspV 2481 TTCGAA TT^CGAA
5184 TTCGAA
PpuMI 6022 AGGACCC [GA]G^G[AT]CC[CT]
7696 AGGTCCT
RcaI 2937 TCATGA T^CATGA
6377 TCATGA
ScaI 32 AGTACT AGT^ACT
8105 AGTACT
SfuI 2481 TTCGAA TT^CGAA
5184 TTCGAA
SpeI 7832 ACTAGT A^CTAGT
7971 ACTAGT
SspI 22 AATATT AAT^ATT
6807 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspHI AspI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BciVI BclI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoRV EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PaeR7I
PflFI PleI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI