##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AatII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
BsiWI BspDI BstBI ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FseI HpaI KspI MluI
NotI NruI NspV PacI PinAI
PmeI PshBI RsrII SacII SbfI
SfiI SfuI SnaBI SpeI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AclI 2439 AACGTT AA^CGTT
AflII 3092 CTTAAG C^TTAAG
Asp700I 3516 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 7325 GGATAC GTATCC(6/5)
BfrI 3092 CTTAAG C^TTAAG
BspLU11I 6902 ACATGT A^CATGT
BssHII 35 GCGCGC G^CGCGC
BssSI 990 CTCGTG CACGAG(-5/-1)
Bst1107I 3338 GTATAC GTA^TAC
BstEII 401 GGTGACC G^GT[ACGT]ACC
BstPI 401 GGTGACC G^GT[ACGT]ACC
BstZ17I 3338 GTATAC GTA^TAC
EcoO65I 401 GGTGACC G^GT[ACGT]ACC
MfeI 7163 CAATTG C^AATTG
MunI 7163 CAATTG C^AATTG
NdeI 3382 CATATG CA^TATG
PaeR7I 834 CTCGAG C^TCGAG
Psp1406I 2439 AACGTT AA^CGTT
PvuI 5 CGATCG CGAT^CG
SalI 7110 GTCGAC G^TCGAC
ScaI 3151 AGTACT AGT^ACT
SexAI 2326 ACCAGGT A^CC[AT]GGT
SphI 5114 GCATGC GCATG^C
XhoI 834 CTCGAG C^TCGAG
XmnI 3516 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1130 GGTACC G^GTACC
2539 GGTACC
AccI 3338 GTATAC GT^[AC][GT]AC
7110 GTCGAC
AccIII 601 TCCGGA T^CCGGA
4102 TCCGGA
ApaI 1297 GGGCCC GGGCC^C
1476 GGGCCC
Asp718I 1130 GGTACC G^GTACC
2539 GGTACC
AspI 1230 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5380 GACATCGTC
AvrII 1287 CCTAGG C^CTAGG
5591 CCTAGG
BbrPI 4569 CACGTG CAC^GTG
5488 CACGTG
BbvCI 3716 CCTCAGC CCTCAGC(-5/-2)
5143 GCTGAGG
BcgI 5 CGATCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3802 CGAGGTGGCTGC
BlnI 1287 CCTAGG C^CTAGG
5591 CCTAGG
BlpI 1459 GCTCAGC GC^T[ACGT]AGC
6557 GCTGAGC
Bpu1102I 1459 GCTCAGC GC^T[ACGT]AGC
6557 GCTGAGC
BsaAI 4569 CACGTG [CT]AC^GT[GA]
5488 CACGTG
BsaBI 3254 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5609 GATAATCATC
BsaI 4274 GAGACC GGTCTC(1/5)
6332 GGTCTC
BsaWI 601 TCCGGA [AT]^CCGG[AT]
4102 TCCGGA
BseAI 601 TCCGGA T^CCGGA
4102 TCCGGA
BsiEI 5 CGATCG CG[GA][CT]^CG
17 CGGTCG
BsmI 2180 GAATGC GAATGC(1/-1)
220 GCATTC
BspEI 601 TCCGGA T^CCGGA
4102 TCCGGA
CelII 1459 GCTCAGC GC^T[ACGT]AGC
6557 GCTGAGC
DraIII 1852 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
2325 CACCAGGTG
EcoRI 649 GAATTC G^AATTC
1766 GAATTC
EcoRV 3495 GATATC GAT^ATC
5756 GATATC
KpnI 1130 GGTACC GGTAC^C
2539 GGTACC
MamI 3254 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5609 GATAATCATC
MroI 601 TCCGGA T^CCGGA
4102 TCCGGA
NheI 22 GCTAGC G^CTAGC
2996 GCTAGC
PflFI 1230 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5380 GACATCGTC
PmaCI 4569 CACGTG CAC^GTG
5488 CACGTG
PmlI 4569 CACGTG CAC^GTG
5488 CACGTG
PshAI 2876 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7818 GACCCAAGTC
SgrAI 85 CGCCGGCG C[GA]^CCGG[CT]G
2415 CGCCGGCG
SmaI 1716 CCCGGG CCC^GGG
3167 CCCGGG
Tth111I 1230 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5380 GACATCGTC
XbaI 5670 TCTAGA T^CTAGA
7515 TCTAGA
XcmI 553 CCATGGTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3391 CCATGAAGAATGTGG
XmaCI 1716 CCCGGG C^CCGGG
3167 CCCGGG
XmaI 1716 CCCGGG C^CCGGG
3167 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII AviII
BalI BamHI BanI BanII BbeI
BbsI BbvI BclI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII