##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI ApaI AscI AspEI BbeI
BsaBI BsaWI BseAI BsiEI BsiWI
BspEI BspHI BsrFI BssHII Cfr10I
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
KasI MamI MfeI MluI MroI
MunI NaeI NarI NgoMIV NotI
NruI NsiI PaeR7I PinAI PmeI
Psp1406I PvuI RcaI RsrII SfiI
SfoI SgrAI SnaBI SpeI SphI
XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 4217 GGTACC G^GTACC
AcyI 4974 GGCGTC G[GA]^CG[CT]C
Alw44I 7103 GTGCAC G^TGCAC
ApaLI 7103 GTGCAC G^TGCAC
Asp718I 4217 GGTACC G^GTACC
AspI 7312 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3677 CCTAGG C^CTAGG
BamHI 3622 GGATCC G^GATCC
BbvCI 4143 GCTGAGG CCTCAGC(-5/-2)
BglI 2859 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 6629 AGATCT A^GATCT
BlnI 3677 CCTAGG C^CTAGG
BsaHI 4974 GGCGTC G[GA]^CG[CT]C
BssSI 4358 CACGAG CACGAG(-5/-1)
BstBI 4597 TTCGAA TT^CGAA
EcoNI 2791 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2876 GAATTC G^AATTC
Hin1I 4974 GGCGTC G[GA]^CG[CT]C
HpaI 2310 GTTAAC GTT^AAC
KpnI 4217 GGTACC GGTAC^C
KspI 5316 CCGCGG CCGC^GG
NdeI 1605 CATATG CA^TATG
NheI 7510 GCTAGC G^CTAGC
NspV 4597 TTCGAA TT^CGAA
PflFI 7312 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 5316 CCGCGG CCGC^GG
SbfI 3635 CCTGCAGG CCTGCA^GG
SexAI 2842 ACCAGGT A^CC[AT]GGT
SfuI 4597 TTCGAA TT^CGAA
SmaI 1663 CCCGGG CCC^GGG
Sse8387I 3635 CCTGCAGG CCTGCA^GG
SwaI 7297 ATTTAAAT ATTT^AAAT
Tth111I 7312 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6307 TCTAGA T^CTAGA
XcmI 4357 CCACGAGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1663 CCCGGG C^CCGGG
XmaI 1663 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflII 3821 CTTAAG C^TTAAG
7090 CTTAAG
AviII 1166 TGCGCA TGC^GCA
3340 TGCGCA
BbrPI 276 CACGTG CAC^GTG
1080 CACGTG
BfrI 3821 CTTAAG C^TTAAG
7090 CTTAAG
BsaAI 276 CACGTG [CT]AC^GT[GA]
1080 CACGTG
BspDI 924 ATCGAT AT^CGAT
4455 ATCGAT
BspLU11I 1355 ACATGT A^CATGT
2116 ACATGT
Bst1107I 3999 GTATAC GTA^TAC
7242 GTATAC
BstEII 2081 GGTTACC G^GT[ACGT]ACC
2507 GGTCACC
BstPI 2081 GGTTACC G^GT[ACGT]ACC
2507 GGTCACC
BstZ17I 3999 GTATAC GTA^TAC
7242 GTATAC
ClaI 924 ATCGAT AT^CGAT
4455 ATCGAT
EcoO65I 2081 GGTTACC G^GT[ACGT]ACC
2507 GGTCACC
FspI 1166 TGCGCA TGC^GCA
3340 TGCGCA
HaeII 1883 GGCGCT [GA]GCGC^[CT]
3079 AGCGCC
HgaI 2456 GCGTC GACGC(5/10)
4975 GCGTC
NcoI 6499 CCATGG C^CATGG
7795 CCATGG
PacI 6329 TTAATTAA TTAAT^TAA
7902 TTAATTAA
PmaCI 276 CACGTG CAC^GTG
1080 CACGTG
PmlI 276 CACGTG CAC^GTG
1080 CACGTG
PpuMI 309 GGGTCCT [GA]G^G[AT]CC[CT]
2644 AGGACCT
PshAI 4626 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6903 GACCACGGTC
SacI 5309 GAGCTC GAGCT^C
7628 GAGCTC
SalI 2478 GTCGAC G^TCGAC
3961 GTCGAC
SapI 5283 GAAGAGC GCTCTTC(1/4)
348 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcgI BciVI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI