##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV FbaI FseI FspI HindIII
HpaI KpnI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SacI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AclI 860 AACGTT AA^CGTT
AcsI 1992 GAATTT [GA]^AATT[CT]
AflIII 112 ACACGT A^C[GA][CT]GT
AhdI 2018 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1992 GAATTT [GA]^AATT[CT]
AspEI 2018 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1661 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 57 TGGCCA TGG^CCA
BbvCI 1121 CCTCAGC CCTCAGC(-5/-2)
BglI 1041 GCCGGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 358 TACGTG [CT]AC^GT[GA]
BsaBI 298 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 841 TCCGGT [AT]^CCGG[AT]
BsgI 1149 CTGCAC GTGCAG(16/14)
BsmBI 1659 GAGACG CGTCTC(1/5)
Bsp1407I 2134 TGTACA T^GTACA
BsrBI 722 GAGCGG CCGCTC(-3/-3)
BsrGI 2134 TGTACA T^GTACA
BssSI 851 CACGAG CACGAG(-5/-1)
BstXI 1377 CCAAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2445 TTTAAA TTT^AAA
Eam1105I 2018 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 393 CCAAGG C^C[AT][AT]GG
EcoT22I 2006 ATGCAT ATGCA^T
HincII 1112 GTCGAC GT[CT]^[GA]AC
HindII 1112 GTCGAC GT[CT]^[GA]AC
KspI 821 CCGCGG CCGC^GG
MamI 298 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 57 TGGCCA TGG^CCA
MscI 57 TGGCCA TGG^CCA
NaeI 99 GCCGGC GCC^GGC
NgoMIV 99 GCCGGC G^CCGGC
NsiI 2006 ATGCAT ATGCA^T
PflFI 1661 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 354 CCACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 614 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 860 AACGTT AA^CGTT
PvuII 738 CAGCTG CAG^CTG
SacII 821 CCGCGG CCGC^GG
SalI 1112 GTCGAC G^TCGAC
SmaI 426 CCCGGG CCC^GGG
SspBI 2134 TGTACA T^GTACA
StuI 1118 AGGCCT AGG^CCT
StyI 393 CCAAGG C^C[AT][AT]GG
Tth111I 1661 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 354 CCACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 426 CCCGGG C^CCGGG
XmaI 426 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 1514 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1772 CAGCCACTG
ApaI 131 GGGCCC GGGCC^C
388 GGGCCC
AvaI 426 CCCGGG C^[CT]CG[GA]G
1625 CCCGAG
BbeI 96 GGCGCC GGCGC^C
162 GGCGCC
BbsI 904 GAAGAC GAAGAC(2/6)
1552 GAAGAC
BfaI 438 CTAG C^TAG
453 CTAG
BpuAI 904 GAAGAC GAAGAC(2/6)
1552 GAAGAC
BsoBI 426 CCCGGG C^[CT]CG[GA]G
1625 CCCGAG
BspMI 247 GCAGGT ACCTGC(4/8)
535 GCAGGT
BstEII 266 GGTGACC G^GT[ACGT]ACC
1925 GGTAACC
BstPI 266 GGTGACC G^GT[ACGT]ACC
1925 GGTAACC
BstYI 963 AGATCC [GA]^GATC[CT]
1846 AGATCC
EcoNI 786 CCTCCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1501 CCTGGAGAAGG
EcoO65I 266 GGTGACC G^GT[ACGT]ACC
1925 GGTAACC
KasI 96 GGCGCC G^GCGCC
162 GGCGCC
MaeI 438 CTAG C^TAG
453 CTAG
MflI 963 AGATCC [GA]^GATC[CT]
1846 AGATCC
NarI 96 GGCGCC GG^CGCC
162 GGCGCC
NspI 636 GCATGC [GA]CATG^[CT]
2004 GCATGC
SfoI 96 GGCGCC GGC^GCC
162 GGCGCC
SphI 636 GCATGC GCATG^C
2004 GCATGC
XcmI 240 CCATGATGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1593 CCAAAGAGCTGCTGG
XhoII 963 AGATCC [GA]^GATC[CT]
1846 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI ApaLI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I