##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbeI
BbrPI BbvCI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I CelII CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FbaI FspI
HindIII HpaI KasI KpnI MamI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 740 GACGTC GACGT^C
AccI 791 GTCTAC GT^[AC][GT]AC
AcsI 2886 GAATTT [GA]^AATT[CT]
AcyI 740 GACGTC G[GA]^CG[CT]C
AflIII 777 ACACGT A^C[GA][CT]GT
Alw44I 1667 GTGCAC G^TGCAC
ApaLI 1667 GTGCAC G^TGCAC
ApoI 2886 GAATTT [GA]^AATT[CT]
Asp700I 989 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1333 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 911 GGCACC G^G[CT][GA]CC
BciVI 2419 GTATCC GTATCC(6/5)
BglI 53 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2172 AGATCT A^GATCT
BsaAI 778 CACGTA [CT]AC^GT[GA]
BsaHI 740 GACGTC G[GA]^CG[CT]C
BsaI 1517 GAGACC GGTCTC(1/5)
BsgI 2003 CTGCAC GTGCAG(16/14)
BsmI 1216 GAATGC GAATGC(1/-1)
BspDI 1463 ATCGAT AT^CGAT
BspHI 1308 TCATGA T^CATGA
BstAPI 2620 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 1463 ATCGAT AT^CGAT
EcoT22I 746 ATGCAT ATGCA^T
FseI 1876 GGCCGGCC GGCCGG^CC
HaeII 951 GGCGCT [GA]GCGC^[CT]
Hin1I 740 GACGTC G[GA]^CG[CT]C
KspI 57 CCGCGG CCGC^GG
NaeI 1877 GCCGGC GCC^GGC
NgoMIV 1877 GCCGGC G^CCGGC
NsiI 746 ATGCAT ATGCA^T
PaeR7I 2612 CTCGAG C^TCGAG
PflFI 1333 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1906 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1308 TCATGA T^CATGA
SacII 57 CCGCGG CCGC^GG
SmaI 1830 CCCGGG CCC^GGG
SmlI 2612 CTCGAG C^T[CT][GA]AG
StuI 2647 AGGCCT AGG^CCT
Tth111I 1333 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2612 CTCGAG C^TCGAG
XmaCI 1830 CCCGGG C^CCGGG
XmaI 1830 CCCGGG C^CCGGG
XmnI 989 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 1264 GTAC GT^AC
1885 GTAC
BalI 804 TGGCCA TGG^CCA
966 TGGCCA
BsrBI 2032 CCGCTC CCGCTC(-3/-3)
2653 CCGCTC
BsrFI 1877 GCCGGC [GA]^CCGG[CT]
2832 GCCGGT
BstEII 457 GGTGACC G^GT[ACGT]ACC
2679 GGTCACC
BstPI 457 GGTGACC G^GT[ACGT]ACC
2679 GGTCACC
Cfr10I 1877 GCCGGC [GA]^CCGG[CT]
2832 GCCGGT
EarI 1259 GAAGAG CTCTTC(1/4)
3049 GAAGAG
EcoO65I 457 GGTGACC G^GT[ACGT]ACC
2679 GGTCACC
Ksp632I 1259 GAAGAG CTCTTC(1/4)
3049 GAAGAG
MluNI 804 TGGCCA TGG^CCA
966 TGGCCA
MscI 804 TGGCCA TGG^CCA
966 TGGCCA
MseI 1580 TTAA T^TAA
2058 TTAA
NspI 1456 GCATGT [GA]CATG^[CT]
2415 GCATGT
PpuMI 972 AGGACCT [GA]G^G[AT]CC[CT]
2202 AGGACCT
RsaI 1264 GTAC GT^AC
1885 GTAC
TfiI 1272 GATTC G^A[AT]TC
2250 GATTC
Tru9I 1580 TTAA T^TAA
2058 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BamHI BanII BbsI BbvI BcgI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII