##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
AscI Asp718I AspEI AvrII BbeI
BbrPI BciVI BfrI BlnI BlpI
Bpu1102I BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
FseI HpaI KasI KpnI KspI
MfeI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PvuI RsrII SacII SalI
SbfI SfoI SfuI SgrAI SnaBI
Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2240 GTAGAC GT^[AC][GT]AC
AccII 5 CGCG CG^CG
AclI 2432 AACGTT AA^CGTT
Alw44I 5476 GTGCAC G^TGCAC
AocI 1914 CCTGAGG CC^T[ACGT]AGG
Aor51HI 6933 AGCGCT AGC^GCT
ApaLI 5476 GTGCAC G^TGCAC
BcgI 460 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 4082 CACGTA [CT]AC^GT[GA]
BsaWI 2642 TCCGGT [AT]^CCGG[AT]
BsmI 4144 GCATTC GAATGC(1/-1)
BspHI 3311 TCATGA T^CATGA
BstUI 5 CGCG CG^CG
Bsu36I 1914 CCTGAGG CC^T[ACGT]AGG
Eco47III 6933 AGCGCT AGC^GCT
Eco81I 1914 CCTGAGG CC^T[ACGT]AGG
HgaI 3 GACGC GACGC(5/10)
MluI 4 ACGCGT A^CGCGT
MvnI 5 CGCG CG^CG
PshAI 7032 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2432 AACGTT AA^CGTT
RcaI 3311 TCATGA T^CATGA
SapI 1646 GCTCTTC GCTCTTC(1/4)
ScaI 7129 AGTACT AGT^ACT
SexAI 1427 ACCAGGT A^CC[AT]GGT
SfiI 7227 GGCCCTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 4008 ACTAGT A^CTAGT
SphI 5741 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 5132 GACGTC GACGT^C
6901 GACGTC
AcyI 5132 GACGTC G[GA]^CG[CT]C
6901 GACGTC
ApaI 1120 GGGCCC GGGCC^C
4614 GGGCCC
Asp700I 2004 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2292 GAAGCTGTTC
AspI 378 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3099 GACAGAGTC
AviII 4108 TGCGCA TGC^GCA
5935 TGCGCA
BamHI 3754 GGATCC G^GATCC
6653 GGATCC
BclI 1276 TGATCA T^GATCA
4790 TGATCA
BsaBI 1296 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4319 GATAGTGATC
BsaHI 5132 GACGTC G[GA]^CG[CT]C
6901 GACGTC
BsmBI 5134 CGTCTC CGTCTC(1/5)
6821 GAGACG
BspLU11I 3907 ACATGT A^CATGT
4339 ACATGT
BsrBI 5712 GAGCGG CCGCTC(-3/-3)
6244 GAGCGG
DraIII 5796 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
6388 CACTGCGTG
EcoRI 2110 GAATTC G^AATTC
2178 GAATTC
EcoRV 5187 GATATC GAT^ATC
6010 GATATC
FbaI 1276 TGATCA T^GATCA
4790 TGATCA
FspI 4108 TGCGCA TGC^GCA
5935 TGCGCA
Hin1I 5132 GACGTC G[GA]^CG[CT]C
6901 GACGTC
HindIII 4699 AAGCTT A^AGCTT
5832 AAGCTT
MamI 1296 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4319 GATAGTGATC
NdeI 5992 CATATG CA^TATG
6030 CATATG
PflFI 378 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3099 GACAGAGTC
TaqI 5317 TCGA T^CGA
6361 TCGA
Tth111I 378 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3099 GACAGAGTC
TthHB8I 5317 TCGA T^CGA
6361 TCGA
XmnI 2004 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2292 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PshBI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XbaI
XcmI XhoII XmaCI XmaI