##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaLI AviII AvrII BamHI BbrPI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BspDI BspEI CelII
ClaI CpoI DrdI EagI EclXI
Eco52I FseI FspI HgaI HincII
HindII HindIII HpaI MamI MfeI
MluI MroI MunI NdeI NotI
NruI PacI PinAI PmaCI PmlI
PshAI Psp1406I PvuI RsrII SacI
SalI SgrAI SnaBI SpeI SphI
XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1600 GGTACC G^GTACC
AccI 1264 GTATAC GT^[AC][GT]AC
AflIII 1689 ACATGT A^C[GA][CT]GT
AhdI 2895 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5905 AGCGCT AGC^GCT
AscI 28 GGCGCGCC GG^CGCGCC
AseI 6771 ATTAAT AT^TAAT
AsnI 6771 ATTAAT AT^TAAT
Asp718I 1600 GGTACC G^GTACC
AspEI 2895 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 2452 GTATCC GTATCC(6/5)
BclI 7196 TGATCA T^GATCA
BglII 6589 AGATCT A^GATCT
BsmBI 2969 CGTCTC CGTCTC(1/5)
BspLU11I 1689 ACATGT A^CATGT
BspMI 3503 GCAGGT ACCTGC(4/8)
Bst1107I 1264 GTATAC GTA^TAC
BstBI 4381 TTCGAA TT^CGAA
BstZ17I 1264 GTATAC GTA^TAC
Eam1105I 2895 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5905 AGCGCT AGC^GCT
EcoRI 3511 GAATTC G^AATTC
EcoRV 4193 GATATC GAT^ATC
EcoT22I 2220 ATGCAT ATGCA^T
FbaI 7196 TGATCA T^GATCA
KpnI 1600 GGTACC GGTAC^C
NaeI 102 GCCGGC GCC^GGC
NgoMIV 102 GCCGGC G^CCGGC
NsiI 2220 ATGCAT ATGCA^T
NspV 4381 TTCGAA TT^CGAA
PaeR7I 70 CTCGAG C^TCGAG
PflMI 5128 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 6853 GTTTAAAC GTTT^AAAC
PshBI 6771 ATTAAT AT^TAAT
SapI 5576 GAAGAGC GCTCTTC(1/4)
SfuI 4381 TTCGAA TT^CGAA
SspI 4361 AATATT AAT^ATT
SwaI 6621 ATTTAAAT ATTT^AAAT
Van91I 5128 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 70 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 6326 CTTAAG C^TTAAG
7406 CTTAAG
Asp700I 600 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2537 GAAGTGCTTC
AspI 4517 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5356 GACAGAGTC
BcgI 277 GCAGTCCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6163 GCAGTGTGCTCG
BfrI 6326 CTTAAG C^TTAAG
7406 CTTAAG
BsaAI 4547 CACGTA [CT]AC^GT[GA]
7303 TACGTG
BsaWI 3405 ACCGGA [AT]^CCGG[AT]
5415 ACCGGA
BsmI 1072 GAATGC GAATGC(1/-1)
4157 GCATTC
Bsp1407I 5213 TGTACA T^GTACA
7017 TGTACA
BsrFI 102 GCCGGC [GA]^CCGG[CT]
1951 ACCGGC
BsrGI 5213 TGTACA T^GTACA
7017 TGTACA
BssHII 29 GCGCGC G^CGCGC
50 GCGCGC
BssSI 6171 CTCGTG CACGAG(-5/-1)
6234 CTCGTG
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
1951 ACCGGC
DraIII 1097 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
1956 CACAAGGTG
KspI 79 CCGCGG CCGC^GG
3157 CCGCGG
NheI 482 GCTAGC G^CTAGC
2669 GCTAGC
PflFI 4517 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5356 GACAGAGTC
SacII 79 CCGCGG CCGC^GG
3157 CCGCGG
ScaI 1652 AGTACT AGT^ACT
2585 AGTACT
SfiI 603 GGCCTTCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2315 GGCCAGAGTGGCC
SmaI 107 CCCGGG CCC^GGG
2100 CCCGGG
SspBI 5213 TGTACA T^GTACA
7017 TGTACA
StuI 602 AGGCCT AGG^CCT
7133 AGGCCT
Tth111I 4517 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5356 GACAGAGTC
XcmI 3919 CCATGAGACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5124 CCACCCAGCCACTGG
XmaCI 107 CCCGGG C^CCGGG
2100 CCCGGG
XmaI 107 CCCGGG C^CCGGG
2100 CCCGGG
XmnI 600 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2537 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrBI BsrDI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
SbfI ScrFI SexAI SfaNI SfcI
SfoI SmlI Sse8387I StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII