##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccIII AclI AcsI AgeI ApoI
AscI AseI AsnI AspI AvrII
BclI BlnI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BsrGI
BssHII Bst1107I BstZ17I ClaI CpoI
DraI EcoRI FbaI HpaI MfeI
MluI MroI MunI NdeI NotI
NruI PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SgrAI SnaBI SpeI SphI
SspBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 6873 GACGTC GACGT^C
AflIII 6769 ACATGT A^C[GA][CT]GT
Aor51HI 5329 AGCGCT AGC^GCT
Asp700I 7491 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 3412 GGATCC G^GATCC
BbrPI 2695 CACGTG CAC^GTG
BcgI 7902 CGAGGCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3844 AGATCT A^GATCT
BsaBI 7094 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 6769 ACATGT A^CATGT
BstBI 1028 TTCGAA TT^CGAA
Eco47III 5329 AGCGCT AGC^GCT
EcoRV 3054 GATATC GAT^ATC
FseI 2086 GGCCGGCC GGCCGG^CC
HincII 1743 GTCAAC GT[CT]^[GA]AC
HindII 1743 GTCAAC GT[CT]^[GA]AC
MamI 7094 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 7745 GCTAGC G^CTAGC
NspV 1028 TTCGAA TT^CGAA
PmaCI 2695 CACGTG CAC^GTG
PmlI 2695 CACGTG CAC^GTG
SexAI 3964 ACCTGGT A^CC[AT]GGT
SfiI 5819 GGCCTTCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1028 TTCGAA TT^CGAA
SspI 5070 AATATT AAT^ATT
TfiI 5805 GATTC G^A[AT]TC
XbaI 7631 TCTAGA T^CTAGA
XmnI 7491 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 912 GGTACC G^GTACC
2332 GGTACC
AflII 7050 CTTAAG C^TTAAG
7318 CTTAAG
Alw44I 3387 GTGCAC G^TGCAC
3765 GTGCAC
ApaLI 3387 GTGCAC G^TGCAC
3765 GTGCAC
Asp718I 912 GGTACC G^GTACC
2332 GGTACC
AviII 1495 TGCGCA TGC^GCA
4699 TGCGCA
BbeI 3984 GGCGCC GGCGC^C
4682 GGCGCC
BciVI 7166 GTATCC GTATCC(6/5)
7589 GTATCC
BfrI 7050 CTTAAG C^TTAAG
7318 CTTAAG
BsaAI 2695 CACGTG [CT]AC^GT[GA]
4401 TACGTG
BsmBI 408 GAGACG CGTCTC(1/5)
1196 GAGACG
BspHI 6167 TCATGA T^CATGA
7386 TCATGA
BsrDI 3794 CATTGC GCAATG(2/0)
7138 CATTGC
EagI 2085 CGGCCG C^GGCCG
3547 CGGCCG
EclXI 2085 CGGCCG C^GGCCG
3547 CGGCCG
Eco52I 2085 CGGCCG C^GGCCG
3547 CGGCCG
EcoT22I 6208 ATGCAT ATGCA^T
7844 ATGCAT
FspI 1495 TGCGCA TGC^GCA
4699 TGCGCA
KasI 3984 GGCGCC G^GCGCC
4682 GGCGCC
KpnI 912 GGTACC GGTAC^C
2332 GGTACC
NarI 3984 GGCGCC GG^CGCC
4682 GGCGCC
NsiI 6208 ATGCAT ATGCA^T
7844 ATGCAT
PaeR7I 1548 CTCGAG C^TCGAG
4137 CTCGAG
RcaI 6167 TCATGA T^CATGA
7386 TCATGA
SacI 1545 GAGCTC GAGCT^C
5130 GAGCTC
SbfI 353 CCTGCAGG CCTGCA^GG
4289 CCTGCAGG
ScaI 3377 AGTACT AGT^ACT
7063 AGTACT
SfoI 3984 GGCGCC GGC^GCC
4682 GGCGCC
Sse8387I 353 CCTGCAGG CCTGCA^GG
4289 CCTGCAGG
Tsp509I 1386 AATT ^AATT
6112 AATT
XhoI 1548 CTCGAG C^TCGAG
4137 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApaI AspEI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI