##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccIII AcyI AocI ApaI
AscI AviII BamHI BbeI BglI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BssHII BstBI BstEII BstPI
Bsu36I CelII CpoI DrdI EagI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoT22I FseI FspI Hin1I KasI
KspI MamI MluI MroI NarI
NdeI NotI NruI NsiI NspV
PacI PmeI PvuI RcaI RsrII
SacII SapI SbfI SfiI SfoI
SfuI SmaI SphI Sse8387I StuI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2864 GGTACC G^GTACC
AclI 5774 AACGTT AA^CGTT
AflII 2713 CTTAAG C^TTAAG
Alw44I 3339 GTGCAC G^TGCAC
Aor51HI 4030 AGCGCT AGC^GCT
ApaLI 3339 GTGCAC G^TGCAC
Asp718I 2864 GGTACC G^GTACC
AspI 767 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4038 CCTAGG C^CTAGG
BbrPI 2496 CACGTG CAC^GTG
BcgI 5047 CGAAAGAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2069 TGATCA T^GATCA
BfrI 2713 CTTAAG C^TTAAG
BlnI 4038 CCTAGG C^CTAGG
BspDI 533 ATCGAT AT^CGAT
BssSI 2430 CTCGTG CACGAG(-5/-1)
BstXI 1423 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 533 ATCGAT AT^CGAT
DraIII 2493 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1161 CCATGG C^C[GA][CT]GG
Eco47III 4030 AGCGCT AGC^GCT
FbaI 2069 TGATCA T^GATCA
HgaI 6705 GCGTC GACGC(5/10)
KpnI 2864 GGTACC GGTAC^C
NaeI 73 GCCGGC GCC^GGC
NcoI 1161 CCATGG C^CATGG
NgoMIV 73 GCCGGC G^CCGGC
NheI 4140 GCTAGC G^CTAGC
PaeR7I 5598 CTCGAG C^TCGAG
PflFI 767 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2496 CACGTG CAC^GTG
PmlI 2496 CACGTG CAC^GTG
PshAI 422 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5774 AACGTT AA^CGTT
SacI 84 GAGCTC GAGCT^C
SalI 2872 GTCGAC G^TCGAC
SexAI 2867 ACCTGGT A^CC[AT]GGT
SnaBI 6206 TACGTA TAC^GTA
SwaI 1122 ATTTAAAT ATTT^AAAT
Tth111I 767 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5598 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 2925 CGCG CG^CG
5641 CGCG
AgeI 65 ACCGGT A^CCGGT
367 ACCGGT
AhdI 2831 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6305 GACCCCTTGTC
AseI 1044 ATTAAT AT^TAAT
1252 ATTAAT
AsnI 1044 ATTAAT AT^TAAT
1252 ATTAAT
AspEI 2831 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6305 GACCCCTTGTC
BalI 3358 TGGCCA TGG^CCA
4123 TGGCCA
BanI 2640 GGTGCC G^G[CT][GA]CC
2864 GGTACC
BanII 84 GAGCTC G[GA]GC[CT]^C
6671 GAGCCC
BbvCI 5581 CCTCAGC CCTCAGC(-5/-2)
1576 GCTGAGG
BciVI 2230 GTATCC GTATCC(6/5)
3914 GTATCC
BcnI 726 CCCGG CC^[GC]GG
2690 CCGGG
BglII 618 AGATCT A^GATCT
4487 AGATCT
BpmI 1644 CTCCAG CTGGAG(16/14)
156 CTGGAG
BsaAI 2496 CACGTG [CT]AC^GT[GA]
6206 TACGTA
BsmFI 305 GGGAC GGGAC(10/14)
4345 GGGAC
Bst1107I 2897 GTATAC GTA^TAC
3954 GTATAC
BstAPI 2099 GCAGACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5614 GCATTAGCTGC
BstUI 2925 CGCG CG^CG
5641 CGCG
BstZ17I 2897 GTATAC GTA^TAC
3954 GTATAC
Eam1105I 2831 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6305 GACCCCTTGTC
EcoRV 458 GATATC GAT^ATC
4438 GATATC
FauI 13 GCGGG CCCGC(4/6)
22 GCGGG
HpaI 984 GTTAAC GTT^AAC
4169 GTTAAC
MfeI 687 CAATTG C^AATTG
6361 CAATTG
MluNI 3358 TGGCCA TGG^CCA
4123 TGGCCA
MscI 3358 TGGCCA TGG^CCA
4123 TGGCCA
MunI 687 CAATTG C^AATTG
6361 CAATTG
MvnI 2925 CGCG CG^CG
5641 CGCG
NciI 726 CCCGG CC^[GC]GG
2690 CCGGG
PflMI 2492 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3429 CCAAGGGCTGG
PinAI 65 ACCGGT A^CCGGT
367 ACCGGT
PshBI 1044 ATTAAT AT^TAAT
1252 ATTAAT
SgrAI 64 CACCGGTG C[GA]^CCGG[CT]G
366 CACCGGTG
SpeI 172 ACTAGT A^CTAGT
1874 ACTAGT
Van91I 2492 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3429 CCAAGGGCTGG
XcmI 2343 CCAACTGTTAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5697 CCACCTTTGTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BbsI BbvI
BfaI BmyI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmI BsoBI Bsp1286I
Bsp1407I BspLU11I BspMI BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI