################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII AccIII AcyI AocI ApaI AscI AviII BamHI BbeI BglI BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BssHII BstBI BstEII BstPI Bsu36I CelII CpoI DrdI EagI EclXI Eco52I Eco81I EcoNI EcoO65I EcoT22I FseI FspI Hin1I KasI KspI MamI MluI MroI NarI NdeI NotI NruI NsiI NspV PacI PmeI PvuI RcaI RsrII SacII SapI SbfI SfiI SfoI SfuI SmaI SphI Sse8387I StuI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 2864 GGTACC G^GTACC AclI 5774 AACGTT AA^CGTT AflII 2713 CTTAAG C^TTAAG Alw44I 3339 GTGCAC G^TGCAC Aor51HI 4030 AGCGCT AGC^GCT ApaLI 3339 GTGCAC G^TGCAC Asp718I 2864 GGTACC G^GTACC AspI 767 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4038 CCTAGG C^CTAGG BbrPI 2496 CACGTG CAC^GTG BcgI 5047 CGAAAGAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2069 TGATCA T^GATCA BfrI 2713 CTTAAG C^TTAAG BlnI 4038 CCTAGG C^CTAGG BspDI 533 ATCGAT AT^CGAT BssSI 2430 CTCGTG CACGAG(-5/-1) BstXI 1423 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 533 ATCGAT AT^CGAT DraIII 2493 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1161 CCATGG C^C[GA][CT]GG Eco47III 4030 AGCGCT AGC^GCT FbaI 2069 TGATCA T^GATCA HgaI 6705 GCGTC GACGC(5/10) KpnI 2864 GGTACC GGTAC^C NaeI 73 GCCGGC GCC^GGC NcoI 1161 CCATGG C^CATGG NgoMIV 73 GCCGGC G^CCGGC NheI 4140 GCTAGC G^CTAGC PaeR7I 5598 CTCGAG C^TCGAG PflFI 767 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2496 CACGTG CAC^GTG PmlI 2496 CACGTG CAC^GTG PshAI 422 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 5774 AACGTT AA^CGTT SacI 84 GAGCTC GAGCT^C SalI 2872 GTCGAC G^TCGAC SexAI 2867 ACCTGGT A^CC[AT]GGT SnaBI 6206 TACGTA TAC^GTA SwaI 1122 ATTTAAAT ATTT^AAAT Tth111I 767 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 5598 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccII 2925 CGCG CG^CG 5641 CGCG AgeI 65 ACCGGT A^CCGGT 367 ACCGGT AhdI 2831 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6305 GACCCCTTGTC AseI 1044 ATTAAT AT^TAAT 1252 ATTAAT AsnI 1044 ATTAAT AT^TAAT 1252 ATTAAT AspEI 2831 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6305 GACCCCTTGTC BalI 3358 TGGCCA TGG^CCA 4123 TGGCCA BanI 2640 GGTGCC G^G[CT][GA]CC 2864 GGTACC BanII 84 GAGCTC G[GA]GC[CT]^C 6671 GAGCCC BbvCI 5581 CCTCAGC CCTCAGC(-5/-2) 1576 GCTGAGG BciVI 2230 GTATCC GTATCC(6/5) 3914 GTATCC BcnI 726 CCCGG CC^[GC]GG 2690 CCGGG BglII 618 AGATCT A^GATCT 4487 AGATCT BpmI 1644 CTCCAG CTGGAG(16/14) 156 CTGGAG BsaAI 2496 CACGTG [CT]AC^GT[GA] 6206 TACGTA BsmFI 305 GGGAC GGGAC(10/14) 4345 GGGAC Bst1107I 2897 GTATAC GTA^TAC 3954 GTATAC BstAPI 2099 GCAGACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5614 GCATTAGCTGC BstUI 2925 CGCG CG^CG 5641 CGCG BstZ17I 2897 GTATAC GTA^TAC 3954 GTATAC Eam1105I 2831 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6305 GACCCCTTGTC EcoRV 458 GATATC GAT^ATC 4438 GATATC FauI 13 GCGGG CCCGC(4/6) 22 GCGGG HpaI 984 GTTAAC GTT^AAC 4169 GTTAAC MfeI 687 CAATTG C^AATTG 6361 CAATTG MluNI 3358 TGGCCA TGG^CCA 4123 TGGCCA MscI 3358 TGGCCA TGG^CCA 4123 TGGCCA MunI 687 CAATTG C^AATTG 6361 CAATTG MvnI 2925 CGCG CG^CG 5641 CGCG NciI 726 CCCGG CC^[GC]GG 2690 CCGGG PflMI 2492 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3429 CCAAGGGCTGG PinAI 65 ACCGGT A^CCGGT 367 ACCGGT PshBI 1044 ATTAAT AT^TAAT 1252 ATTAAT SgrAI 64 CACCGGTG C[GA]^CCGG[CT]G 366 CACCGGTG SpeI 172 ACTAGT A^CTAGT 1874 ACTAGT Van91I 2492 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3429 CCAAGGGCTGG XcmI 2343 CCAACTGTTAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5697 CCACCTTTGTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BbsI BbvI BfaI BmyI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI