##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
AviII AvrII BbrPI BfrI BlnI
BsaBI BsiWI BspDI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EcoT22I FspI HindIII HpaI MamI
MluI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SfiI SfuI SgrAI
SnaBI SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 2166 TCCGGA T^CCGGA
AflIII 1610 ACATGT A^C[GA][CT]GT
Aor51HI 1469 AGCGCT AGC^GCT
Asp700I 606 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 3794 GGATAC GTATCC(6/5)
BclI 5821 TGATCA T^GATCA
BglII 803 AGATCT A^GATCT
BsaAI 4301 TACGTG [CT]AC^GT[GA]
BseAI 2166 TCCGGA T^CCGGA
Bsp1407I 659 TGTACA T^GTACA
BspEI 2166 TCCGGA T^CCGGA
BspLU11I 1610 ACATGT A^CATGT
BsrBI 142 CCGCTC CCGCTC(-3/-3)
BsrGI 659 TGTACA T^GTACA
BssSI 1874 CTCGTG CACGAG(-5/-1)
BstAPI 5416 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 6146 GACCGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1469 AGCGCT AGC^GCT
EcoRI 73 GAATTC G^AATTC
EcoRV 4050 GATATC GAT^ATC
FbaI 5821 TGATCA T^GATCA
FseI 212 GGCCGGCC GGCCGG^CC
HincII 884 GTTGAC GT[CT]^[GA]AC
HindII 884 GTTGAC GT[CT]^[GA]AC
KspI 5403 CCGCGG CCGC^GG
MroI 2166 TCCGGA T^CCGGA
PaeR7I 1281 CTCGAG C^TCGAG
PshAI 2142 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 5403 CCGCGG CCGC^GG
SphI 1301 GCATGC GCATG^C
SspBI 659 TGTACA T^GTACA
XhoI 1281 CTCGAG C^TCGAG
XmnI 606 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 1005 GGTACC G^GTACC
5718 GGTACC
Asp718I 1005 GGTACC G^GTACC
5718 GGTACC
AspI 160 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2956 GACTCAGTC
BglI 2389 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3461 GCCTGCCAGGC
BsaWI 2166 TCCGGA [AT]^CCGG[AT]
4460 ACCGGA
BsmI 2561 GCATTC GAATGC(1/-1)
3473 GCATTC
BspHI 326 TCATGA T^CATGA
4040 TCATGA
DraIII 4851 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
6994 CACGCAGTG
EagI 11 CGGCCG C^GGCCG
80 CGGCCG
EclXI 11 CGGCCG C^GGCCG
80 CGGCCG
Eco52I 11 CGGCCG C^GGCCG
80 CGGCCG
KpnI 1005 GGTACC GGTAC^C
5718 GGTACC
MfeI 2868 CAATTG C^AATTG
3870 CAATTG
MunI 2868 CAATTG C^AATTG
3870 CAATTG
NcoI 29 CCATGG C^CATGG
722 CCATGG
NspI 1301 GCATGC [GA]CATG^[CT]
1610 ACATGT
PflFI 160 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2956 GACTCAGTC
RcaI 326 TCATGA T^CATGA
4040 TCATGA
SapI 6593 GAAGAGC GCTCTTC(1/4)
3624 GCTCTTC
ScaI 4483 AGTACT AGT^ACT
6696 AGTACT
Tth111I 160 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2956 GACTCAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApaI ApoI AspEI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbsI BbvCI BbvI BcgI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
SbfI ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI Sse8387I StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI