################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII AccI AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI AviII AvrII BbrPI BfrI BlnI BsaBI BsiWI BspDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI EcoT22I FspI HindIII HpaI MamI MluI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccIII 2166 TCCGGA T^CCGGA AflIII 1610 ACATGT A^C[GA][CT]GT Aor51HI 1469 AGCGCT AGC^GCT Asp700I 606 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BciVI 3794 GGATAC GTATCC(6/5) BclI 5821 TGATCA T^GATCA BglII 803 AGATCT A^GATCT BsaAI 4301 TACGTG [CT]AC^GT[GA] BseAI 2166 TCCGGA T^CCGGA Bsp1407I 659 TGTACA T^GTACA BspEI 2166 TCCGGA T^CCGGA BspLU11I 1610 ACATGT A^CATGT BsrBI 142 CCGCTC CCGCTC(-3/-3) BsrGI 659 TGTACA T^GTACA BssSI 1874 CTCGTG CACGAG(-5/-1) BstAPI 5416 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 6146 GACCGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1469 AGCGCT AGC^GCT EcoRI 73 GAATTC G^AATTC EcoRV 4050 GATATC GAT^ATC FbaI 5821 TGATCA T^GATCA FseI 212 GGCCGGCC GGCCGG^CC HincII 884 GTTGAC GT[CT]^[GA]AC HindII 884 GTTGAC GT[CT]^[GA]AC KspI 5403 CCGCGG CCGC^GG MroI 2166 TCCGGA T^CCGGA PaeR7I 1281 CTCGAG C^TCGAG PshAI 2142 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 5403 CCGCGG CCGC^GG SphI 1301 GCATGC GCATG^C SspBI 659 TGTACA T^GTACA XhoI 1281 CTCGAG C^TCGAG XmnI 606 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Acc65I 1005 GGTACC G^GTACC 5718 GGTACC Asp718I 1005 GGTACC G^GTACC 5718 GGTACC AspI 160 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2956 GACTCAGTC BglI 2389 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3461 GCCTGCCAGGC BsaWI 2166 TCCGGA [AT]^CCGG[AT] 4460 ACCGGA BsmI 2561 GCATTC GAATGC(1/-1) 3473 GCATTC BspHI 326 TCATGA T^CATGA 4040 TCATGA DraIII 4851 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 6994 CACGCAGTG EagI 11 CGGCCG C^GGCCG 80 CGGCCG EclXI 11 CGGCCG C^GGCCG 80 CGGCCG Eco52I 11 CGGCCG C^GGCCG 80 CGGCCG KpnI 1005 GGTACC GGTAC^C 5718 GGTACC MfeI 2868 CAATTG C^AATTG 3870 CAATTG MunI 2868 CAATTG C^AATTG 3870 CAATTG NcoI 29 CCATGG C^CATGG 722 CCATGG NspI 1301 GCATGC [GA]CATG^[CT] 1610 ACATGT PflFI 160 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2956 GACTCAGTC RcaI 326 TCATGA T^CATGA 4040 TCATGA SapI 6593 GAAGAGC GCTCTTC(1/4) 3624 GCTCTTC ScaI 4483 AGTACT AGT^ACT 6696 AGTACT Tth111I 160 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2956 GACTCAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 149 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AhdI AluI AlwI AlwNI AocI ApaI ApoI AspEI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcgI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I SbfI ScrFI SexAI SfaNI SfcI SfoI SmaI SmlI Sse8387I StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI