##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AflII       
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsiEI       BsiWI       
BsmBI       BsmI        BspDI       BspHI       BsrBI       
BsrFI       BssHII      BssSI       BstBI       BstEII      
BstPI       BstUI       Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      EagI        
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FseI        FspI        
HindIII     KasI        KpnI        Ksp632I     KspI        
MluI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SwaI        Tth111I     
Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII        320  TCCGGA               T^CCGGA
AhdI         1516  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI         1883  ATTAAT               AT^TAAT
AsnI         1883  ATTAAT               AT^TAAT
AspEI        1516  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         430  GAGCAC               G[AT]GC[AT]^C
AvaI          877  CCCGGG               C^[CT]CG[GA]G
BanI          827  GGTGCC               G^G[CT][GA]CC
BanII        1747  GAGCCC               G[GA]GC[CT]^C
BbvCI        1096  GCTGAGG              CCTCAGC(-5/-2)
BglII        1087  AGATCT               A^GATCT
BsaBI         414  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         320  TCCGGA               [AT]^CCGG[AT]
BseAI         320  TCCGGA               T^CCGGA
BsgI          724  GTGCAG               GTGCAG(16/14)
BsiHKAI       430  GAGCAC               G[AT]GC[AT]^C
BsoBI         877  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1673  TGTACA               T^GTACA
BspEI         320  TCCGGA               T^CCGGA
BspLU11I      686  ACATGT               A^CATGT
BsrGI        1673  TGTACA               T^GTACA
Bst1107I     1839  GTATAC               GTA^TAC
BstZ17I      1839  GTATAC               GTA^TAC
CfoI          144  GCGC                 GCG^C
DrdI          622  GACTTTGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1516  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1435  CCTGTGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         479  GAATTC               G^AATTC
HaeII         143  AGCGCC               [GA]GCGC^[CT]
HhaI          144  GCGC                 GCG^C
HinP1I        144  GCGC                 G^CGC
HpaI           58  GTTAAC               GTT^AAC
MaeII        1990  ACGT                 A^CGT
MamI          414  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1490  CAATTG               C^AATTG
MroI          320  TCCGGA               T^CCGGA
MunI         1490  CAATTG               C^AATTG
PshBI        1883  ATTAAT               AT^TAAT
PvuII          28  CAGCTG               CAG^CTG
SmaI          877  CCCGGG               CCC^GGG
SmlI          148  CTTGAG               C^T[CT][GA]AG
SphI         1637  GCATGC               GCATG^C
SspBI        1673  TGTACA               T^GTACA
SspI         1341  AATATT               AAT^ATT
TaqI          647  TCGA                 T^CGA
TthHB8I       647  TCGA                 T^CGA
XmaCI         877  CCCGGG               C^CCGGG
XmaI          877  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI           18  GACGCC               G[GA]^CG[CT]C
             1174  GGCGTC              
AflIII        686  ACATGT               A^C[GA][CT]GT
             1990  ACGTGT              
AlwI         1611  GATCC                GGATC(4/5)
              453  GGATC               
AlwNI         346  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              417  CAGTATCTG           
BalI          281  TGGCCA               TGG^CCA
              656  TGGCCA              
BcgI           45  CGACGGCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1205  CGAGTCCCCTGC        
BciVI        1851  GGATAC               GTATCC(6/5)
              780  GTATCC              
BclI         1426  TGATCA               T^GATCA
             1924  TGATCA              
BsaHI          18  GACGCC               G[GA]^CG[CT]C
             1174  GGCGTC              
BspMI         345  GCAGGT               ACCTGC(4/8)
             1114  GCAGGT              
BsrDI         510  CATTGC               GCAATG(2/0)
              230  GCAATG              
BstAPI        345  GCAGGTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1637  GCATGCTTTGC         
BstXI         803  CCAAGGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1164  CCATGCTGCTGG        
BstYI        1087  AGATCT               [GA]^GATC[CT]
             1610  AGATCC              
EaeI          281  TGGCCA               [CT]^GGCC[GA]
              656  TGGCCA              
FbaI         1426  TGATCA               T^GATCA
             1924  TGATCA              
HgaI           18  GACGC                GACGC(5/10)
             1175  GCGTC               
Hin1I          18  GACGCC               G[GA]^CG[CT]C
             1174  GGCGTC              
MflI         1087  AGATCT               [GA]^GATC[CT]
             1610  AGATCC              
MluNI         281  TGGCCA               TGG^CCA
              656  TGGCCA              
MscI          281  TGGCCA               TGG^CCA
              656  TGGCCA              
MspA1I         28  CAGCTG               C[AC]G^C[GT]G
              633  CAGCGG              
NcoI          653  CCATGG               C^CATGG
              920  CCATGG              
PpuMI         679  AGGTCCT              [GA]G^G[AT]CC[CT]
              871  AGGACCC             
PstI           53  CTGCAG               CTGCA^G
              343  CTGCAG              
TfiI         1320  GATTC                G^A[AT]TC
             1542  GATTC               
XhoII        1087  AGATCT               [GA]^GATC[CT]
             1610  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
ApoI        AvaII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StuI        StyI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XcmI