##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsiEI BsiWI
BsmBI BsmI BspDI BspHI BsrBI
BsrFI BssHII BssSI BstBI BstEII
BstPI BstUI Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FseI FspI
HindIII KasI KpnI Ksp632I KspI
MluI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Tth111I
Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 320 TCCGGA T^CCGGA
AhdI 1516 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1883 ATTAAT AT^TAAT
AsnI 1883 ATTAAT AT^TAAT
AspEI 1516 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 430 GAGCAC G[AT]GC[AT]^C
AvaI 877 CCCGGG C^[CT]CG[GA]G
BanI 827 GGTGCC G^G[CT][GA]CC
BanII 1747 GAGCCC G[GA]GC[CT]^C
BbvCI 1096 GCTGAGG CCTCAGC(-5/-2)
BglII 1087 AGATCT A^GATCT
BsaBI 414 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 320 TCCGGA [AT]^CCGG[AT]
BseAI 320 TCCGGA T^CCGGA
BsgI 724 GTGCAG GTGCAG(16/14)
BsiHKAI 430 GAGCAC G[AT]GC[AT]^C
BsoBI 877 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1673 TGTACA T^GTACA
BspEI 320 TCCGGA T^CCGGA
BspLU11I 686 ACATGT A^CATGT
BsrGI 1673 TGTACA T^GTACA
Bst1107I 1839 GTATAC GTA^TAC
BstZ17I 1839 GTATAC GTA^TAC
CfoI 144 GCGC GCG^C
DrdI 622 GACTTTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1516 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1435 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 479 GAATTC G^AATTC
HaeII 143 AGCGCC [GA]GCGC^[CT]
HhaI 144 GCGC GCG^C
HinP1I 144 GCGC G^CGC
HpaI 58 GTTAAC GTT^AAC
MaeII 1990 ACGT A^CGT
MamI 414 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1490 CAATTG C^AATTG
MroI 320 TCCGGA T^CCGGA
MunI 1490 CAATTG C^AATTG
PshBI 1883 ATTAAT AT^TAAT
PvuII 28 CAGCTG CAG^CTG
SmaI 877 CCCGGG CCC^GGG
SmlI 148 CTTGAG C^T[CT][GA]AG
SphI 1637 GCATGC GCATG^C
SspBI 1673 TGTACA T^GTACA
SspI 1341 AATATT AAT^ATT
TaqI 647 TCGA T^CGA
TthHB8I 647 TCGA T^CGA
XmaCI 877 CCCGGG C^CCGGG
XmaI 877 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 18 GACGCC G[GA]^CG[CT]C
1174 GGCGTC
AflIII 686 ACATGT A^C[GA][CT]GT
1990 ACGTGT
AlwI 1611 GATCC GGATC(4/5)
453 GGATC
AlwNI 346 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
417 CAGTATCTG
BalI 281 TGGCCA TGG^CCA
656 TGGCCA
BcgI 45 CGACGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1205 CGAGTCCCCTGC
BciVI 1851 GGATAC GTATCC(6/5)
780 GTATCC
BclI 1426 TGATCA T^GATCA
1924 TGATCA
BsaHI 18 GACGCC G[GA]^CG[CT]C
1174 GGCGTC
BspMI 345 GCAGGT ACCTGC(4/8)
1114 GCAGGT
BsrDI 510 CATTGC GCAATG(2/0)
230 GCAATG
BstAPI 345 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1637 GCATGCTTTGC
BstXI 803 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1164 CCATGCTGCTGG
BstYI 1087 AGATCT [GA]^GATC[CT]
1610 AGATCC
EaeI 281 TGGCCA [CT]^GGCC[GA]
656 TGGCCA
FbaI 1426 TGATCA T^GATCA
1924 TGATCA
HgaI 18 GACGC GACGC(5/10)
1175 GCGTC
Hin1I 18 GACGCC G[GA]^CG[CT]C
1174 GGCGTC
MflI 1087 AGATCT [GA]^GATC[CT]
1610 AGATCC
MluNI 281 TGGCCA TGG^CCA
656 TGGCCA
MscI 281 TGGCCA TGG^CCA
656 TGGCCA
MspA1I 28 CAGCTG C[AC]G^C[GT]G
633 CAGCGG
NcoI 653 CCATGG C^CATGG
920 CCATGG
PpuMI 679 AGGTCCT [GA]G^G[AT]CC[CT]
871 AGGACCC
PstI 53 CTGCAG CTGCA^G
343 CTGCAG
TfiI 1320 GATTC G^A[AT]TC
1542 GATTC
XhoII 1087 AGATCT [GA]^GATC[CT]
1610 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AvaII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI