##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AspEI
BcgI BclI BfrI BsaBI BseAI
BsiWI BsmBI BspEI BssHII Bst1107I
BstZ17I CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FbaI
FseI HindIII KspI MamI MluI
MroI NdeI NotI NruI PacI
PinAI PmeI PshAI PvuI RsrII
SacII SalI ScaI SgrAI SnaBI
SpeI SphI SspI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 631 GGTACC G^GTACC
AccII 4739 CGCG CG^CG
AclI 1208 AACGTT AA^CGTT
AcyI 5113 GGCGCC G[GA]^CG[CT]C
ApaI 3785 GGGCCC GGGCC^C
Asp718I 631 GGTACC G^GTACC
AviII 3848 TGCGCA TGC^GCA
AvrII 1197 CCTAGG C^CTAGG
BamHI 914 GGATCC G^GATCC
BbeI 5113 GGCGCC GGCGC^C
BbrPI 3376 CACGTG CAC^GTG
BbsI 4276 GAAGAC GAAGAC(2/6)
BlnI 1197 CCTAGG C^CTAGG
BpuAI 4276 GAAGAC GAAGAC(2/6)
BsaHI 5113 GGCGCC G[GA]^CG[CT]C
BsiEI 6 CGGTCG CG[GA][CT]^CG
BspDI 4204 ATCGAT AT^CGAT
BsrFI 471 GCCGGC [GA]^CCGG[CT]
BssSI 2860 CTCGTG CACGAG(-5/-1)
BstBI 3583 TTCGAA TT^CGAA
BstUI 4739 CGCG CG^CG
Cfr10I 471 GCCGGC [GA]^CCGG[CT]
ClaI 4204 ATCGAT AT^CGAT
FspI 3848 TGCGCA TGC^GCA
HaeII 5113 GGCGCC [GA]GCGC^[CT]
Hin1I 5113 GGCGCC G[GA]^CG[CT]C
HpaI 691 GTTAAC GTT^AAC
KasI 5113 GGCGCC G^GCGCC
KpnI 631 GGTACC GGTAC^C
MvnI 4739 CGCG CG^CG
NaeI 471 GCCGGC GCC^GGC
NarI 5113 GGCGCC GG^CGCC
NgoMIV 471 GCCGGC G^CCGGC
NspV 3583 TTCGAA TT^CGAA
PaeR7I 3268 CTCGAG C^TCGAG
PmaCI 3376 CACGTG CAC^GTG
PmlI 3376 CACGTG CAC^GTG
Psp1406I 1208 AACGTT AA^CGTT
SapI 303 GAAGAGC GCTCTTC(1/4)
SbfI 2883 CCTGCAGG CCTGCA^GG
SfiI 3729 GGCCCGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5113 GGCGCC GGC^GCC
SfuI 3583 TTCGAA TT^CGAA
Sse8387I 2883 CCTGCAGG CCTGCA^GG
XbaI 4944 TCTAGA T^CTAGA
XhoI 3268 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 385 GTCTAC GT^[AC][GT]AC
4828 GTAGAC
AseI 1444 ATTAAT AT^TAAT
2731 ATTAAT
AsnI 1444 ATTAAT AT^TAAT
2731 ATTAAT
BalI 3341 TGGCCA TGG^CCA
5261 TGGCCA
BglI 3730 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5116 GCCTACGAGGC
BglII 2820 AGATCT A^GATCT
3186 AGATCT
BsaAI 571 TACGTG [CT]AC^GT[GA]
3376 CACGTG
BspHI 1415 TCATGA T^CATGA
2624 TCATGA
BspLU11I 2948 ACATGT A^CATGT
3299 ACATGT
BstEII 58 GGTAACC G^GT[ACGT]ACC
165 GGTGACC
BstPI 58 GGTAACC G^GT[ACGT]ACC
165 GGTGACC
DraIII 1025 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
2698 CACCTGGTG
DrdI 220 GACCGAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
412 GACAAGAAAGTC
EcoO65I 58 GGTAACC G^GT[ACGT]ACC
165 GGTGACC
MfeI 2768 CAATTG C^AATTG
3477 CAATTG
MluNI 3341 TGGCCA TGG^CCA
5261 TGGCCA
MscI 3341 TGGCCA TGG^CCA
5261 TGGCCA
MunI 2768 CAATTG C^AATTG
3477 CAATTG
NcoI 2243 CCATGG C^CATGG
5069 CCATGG
NheI 2409 GCTAGC G^CTAGC
2487 GCTAGC
PshBI 1444 ATTAAT AT^TAAT
2731 ATTAAT
RcaI 1415 TCATGA T^CATGA
2624 TCATGA
SexAI 2699 ACCTGGT A^CC[AT]GGT
4658 ACCTGGT
SmaI 2123 CCCGGG CCC^GGG
3731 CCCGGG
XmaCI 2123 CCCGGG C^CCGGG
3731 CCCGGG
XmaI 2123 CCCGGG C^CCGGG
3731 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AspI AvaI AvaII BanI
BanII BbvCI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmnI