##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Aor51HI AscI
Asp718I AspEI BalI BamHI BbeI
BcgI BfrI BlpI Bpu1102I BseAI
BsiWI Bsp1407I BspEI BspHI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
HindIII HpaI KasI KpnI KspI
MluI MluNI MroI MscI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmeI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI StuI SwaI Van91I
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Alw44I 2561 GTGCAC G^TGCAC
ApaI 1419 GGGCCC GGGCC^C
ApaLI 2561 GTGCAC G^TGCAC
AseI 3810 ATTAAT AT^TAAT
AsnI 3810 ATTAAT AT^TAAT
Asp700I 4158 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2168 CCTAGG C^CTAGG
BbrPI 2291 CACGTG CAC^GTG
BclI 3359 TGATCA T^GATCA
BglI 4233 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2168 CCTAGG C^CTAGG
BsaBI 2713 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4995 TCCGGT [AT]^CCGG[AT]
BsiEI 2997 CGGTCG CG[GA][CT]^CG
BspDI 4825 ATCGAT AT^CGAT
BsrBI 4213 GAGCGG CCGCTC(-3/-3)
BstAPI 4429 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 4825 ATCGAT AT^CGAT
DraIII 2649 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 3359 TGATCA T^GATCA
FseI 2408 GGCCGGCC GGCCGG^CC
HaeII 2074 GGCGCT [GA]GCGC^[CT]
HincII 4369 GTCAAC GT[CT]^[GA]AC
HindII 4369 GTCAAC GT[CT]^[GA]AC
MamI 2713 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2884 CAATTG C^AATTG
MunI 2884 CAATTG C^AATTG
NaeI 2409 GCCGGC GCC^GGC
NcoI 4181 CCATGG C^CATGG
NgoMIV 2409 GCCGGC G^CCGGC
PmaCI 2291 CACGTG CAC^GTG
PmlI 2291 CACGTG CAC^GTG
PshAI 2080 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3810 ATTAAT AT^TAAT
SacI 5142 GAGCTC GAGCT^C
ScaI 2965 AGTACT AGT^ACT
SpeI 4573 ACTAGT A^CTAGT
SspI 2976 AATATT AAT^ATT
XmnI 4158 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 4864 GACGCC G[GA]^CG[CT]C
5008 GACGCC
AocI 1758 CCTCAGG CC^T[ACGT]AGG
2204 CCTCAGG
AspI 3253 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3502 GACGGGGTC
BanI 3133 GGTGCC G^G[CT][GA]CC
4998 GGTGCC
BbvCI 3410 CCTCAGC CCTCAGC(-5/-2)
3162 GCTGAGG
BsaHI 4864 GACGCC G[GA]^CG[CT]C
5008 GACGCC
BspLU11I 3764 ACATGT A^CATGT
3852 ACATGT
BsrDI 3370 CATTGC GCAATG(2/0)
1253 GCAATG
BstEII 5106 GGTGACC G^GT[ACGT]ACC
5116 GGTGACC
BstPI 5106 GGTGACC G^GT[ACGT]ACC
5116 GGTGACC
Bsu36I 1758 CCTCAGG CC^T[ACGT]AGG
2204 CCTCAGG
DrdI 4579 GACCTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4752 GACCAAGGTGTC
EarI 4590 CTCTTC CTCTTC(1/4)
4978 GAAGAG
Eco81I 1758 CCTCAGG CC^T[ACGT]AGG
2204 CCTCAGG
EcoO65I 5106 GGTGACC G^GT[ACGT]ACC
5116 GGTGACC
Hin1I 4864 GACGCC G[GA]^CG[CT]C
5008 GACGCC
Ksp632I 4590 CTCTTC CTCTTC(1/4)
4978 GAAGAG
PaeR7I 3419 CTCGAG C^TCGAG
3547 CTCGAG
PflFI 3253 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3502 GACGGGGTC
PvuII 4430 CAGCTG CAG^CTG
4741 CAGCTG
SmaI 2517 CCCGGG CCC^GGG
3186 CCCGGG
Tth111I 3253 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3502 GACGGGGTC
XbaI 1141 TCTAGA T^CTAGA
4306 TCTAGA
XhoI 3419 CTCGAG C^TCGAG
3547 CTCGAG
XmaCI 2517 CCCGGG C^CCGGG
3186 CCCGGG
XmaI 2517 CCCGGG C^CCGGG
3186 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII AviII BanII BbsI
BbvI BciVI BcnI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII