################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Aor51HI AscI Asp718I AspEI BalI BamHI BbeI BcgI BfrI BlpI Bpu1102I BseAI BsiWI Bsp1407I BspEI BspHI BsrGI BssHII Bst1107I BstBI BstZ17I CelII CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I HindIII HpaI KasI KpnI KspI MluI MluNI MroI MscI NarI NdeI NheI NotI NruI NsiI NspV PacI PflMI PinAI PmeI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI StuI SwaI Van91I ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Alw44I 2561 GTGCAC G^TGCAC ApaI 1419 GGGCCC GGGCC^C ApaLI 2561 GTGCAC G^TGCAC AseI 3810 ATTAAT AT^TAAT AsnI 3810 ATTAAT AT^TAAT Asp700I 4158 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 2168 CCTAGG C^CTAGG BbrPI 2291 CACGTG CAC^GTG BclI 3359 TGATCA T^GATCA BglI 4233 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2168 CCTAGG C^CTAGG BsaBI 2713 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4995 TCCGGT [AT]^CCGG[AT] BsiEI 2997 CGGTCG CG[GA][CT]^CG BspDI 4825 ATCGAT AT^CGAT BsrBI 4213 GAGCGG CCGCTC(-3/-3) BstAPI 4429 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 4825 ATCGAT AT^CGAT DraIII 2649 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG FbaI 3359 TGATCA T^GATCA FseI 2408 GGCCGGCC GGCCGG^CC HaeII 2074 GGCGCT [GA]GCGC^[CT] HincII 4369 GTCAAC GT[CT]^[GA]AC HindII 4369 GTCAAC GT[CT]^[GA]AC MamI 2713 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2884 CAATTG C^AATTG MunI 2884 CAATTG C^AATTG NaeI 2409 GCCGGC GCC^GGC NcoI 4181 CCATGG C^CATGG NgoMIV 2409 GCCGGC G^CCGGC PmaCI 2291 CACGTG CAC^GTG PmlI 2291 CACGTG CAC^GTG PshAI 2080 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3810 ATTAAT AT^TAAT SacI 5142 GAGCTC GAGCT^C ScaI 2965 AGTACT AGT^ACT SpeI 4573 ACTAGT A^CTAGT SspI 2976 AATATT AAT^ATT XmnI 4158 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 4864 GACGCC G[GA]^CG[CT]C 5008 GACGCC AocI 1758 CCTCAGG CC^T[ACGT]AGG 2204 CCTCAGG AspI 3253 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3502 GACGGGGTC BanI 3133 GGTGCC G^G[CT][GA]CC 4998 GGTGCC BbvCI 3410 CCTCAGC CCTCAGC(-5/-2) 3162 GCTGAGG BsaHI 4864 GACGCC G[GA]^CG[CT]C 5008 GACGCC BspLU11I 3764 ACATGT A^CATGT 3852 ACATGT BsrDI 3370 CATTGC GCAATG(2/0) 1253 GCAATG BstEII 5106 GGTGACC G^GT[ACGT]ACC 5116 GGTGACC BstPI 5106 GGTGACC G^GT[ACGT]ACC 5116 GGTGACC Bsu36I 1758 CCTCAGG CC^T[ACGT]AGG 2204 CCTCAGG DrdI 4579 GACCTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4752 GACCAAGGTGTC EarI 4590 CTCTTC CTCTTC(1/4) 4978 GAAGAG Eco81I 1758 CCTCAGG CC^T[ACGT]AGG 2204 CCTCAGG EcoO65I 5106 GGTGACC G^GT[ACGT]ACC 5116 GGTGACC Hin1I 4864 GACGCC G[GA]^CG[CT]C 5008 GACGCC Ksp632I 4590 CTCTTC CTCTTC(1/4) 4978 GAAGAG PaeR7I 3419 CTCGAG C^TCGAG 3547 CTCGAG PflFI 3253 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3502 GACGGGGTC PvuII 4430 CAGCTG CAG^CTG 4741 CAGCTG SmaI 2517 CCCGGG CCC^GGG 3186 CCCGGG Tth111I 3253 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3502 GACGGGGTC XbaI 1141 TCTAGA T^CTAGA 4306 TCTAGA XhoI 3419 CTCGAG C^TCGAG 3547 CTCGAG XmaCI 2517 CCCGGG C^CCGGG 3186 CCCGGG XmaI 2517 CCCGGG C^CCGGG 3186 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII AviII BanII BbsI BbvI BciVI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII