##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AgeI AhdI Aor51HI Asp700I
AspEI AvrII BbeI BbrPI BlnI
BsiWI BsmBI Bsp1407I BspDI BsrGI
BstBI BstEII BstPI ClaI CpoI
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV FseI HincII HindII HindIII
HpaI KasI MluI NarI NdeI
NotI NruI NspV PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacI SalI SapI SbfI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 5153 GTATAC GT^[AC][GT]AC
AclI 362 AACGTT AA^CGTT
AcyI 2825 GACGCC G[GA]^CG[CT]C
AflII 4880 CTTAAG C^TTAAG
AflIII 3074 ACATGT A^C[GA][CT]GT
Alw44I 4455 GTGCAC G^TGCAC
ApaLI 4455 GTGCAC G^TGCAC
AscI 996 GGCGCGCC GG^CGCGCC
AseI 5364 ATTAAT AT^TAAT
AsnI 5364 ATTAAT AT^TAAT
AspI 2487 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3465 GGATCC G^GATCC
BciVI 3328 GGATAC GTATCC(6/5)
BclI 3582 TGATCA T^GATCA
BfrI 4880 CTTAAG C^TTAAG
BglII 2418 AGATCT A^GATCT
BsaAI 3874 TACGTG [CT]AC^GT[GA]
BsaHI 2825 GACGCC G[GA]^CG[CT]C
BsmI 1348 GCATTC GAATGC(1/-1)
BspLU11I 3074 ACATGT A^CATGT
Bst1107I 5153 GTATAC GTA^TAC
BstZ17I 5153 GTATAC GTA^TAC
DraI 5095 TTTAAA TTT^AAA
FbaI 3582 TGATCA T^GATCA
Hin1I 2825 GACGCC G[GA]^CG[CT]C
MfeI 5290 CAATTG C^AATTG
MunI 5290 CAATTG C^AATTG
NheI 3790 GCTAGC G^CTAGC
PacI 5409 TTAATTAA TTAAT^TAA
PaeR7I 1003 CTCGAG C^TCGAG
PflFI 2487 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 5364 ATTAAT AT^TAAT
Psp1406I 362 AACGTT AA^CGTT
ScaI 1007 AGTACT AGT^ACT
SexAI 1118 ACCTGGT A^CC[AT]GGT
SfiI 1638 GGCCATGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 944 GCATGC GCATG^C
Tth111I 2487 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1003 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 1190 GGTACC G^GTACC
4668 GGTACC
AcsI 3571 GAATTT [GA]^AATT[CT]
5600 AAATTC
ApoI 3571 GAATTT [GA]^AATT[CT]
5600 AAATTC
Asp718I 1190 GGTACC G^GTACC
4668 GGTACC
AviII 911 TGCGCA TGC^GCA
929 TGCGCA
BsaBI 1410 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1497 GATGAACATC
BsaI 1624 GAGACC GGTCTC(1/5)
269 GGTCTC
BspHI 4689 TCATGA T^CATGA
4927 TCATGA
BspMI 926 ACCTGC ACCTGC(4/8)
1587 GCAGGT
DraIII 1117 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4193 CACCCAGTG
EagI 104 CGGCCG C^GGCCG
1478 CGGCCG
EclXI 104 CGGCCG C^GGCCG
1478 CGGCCG
Eco52I 104 CGGCCG C^GGCCG
1478 CGGCCG
FspI 911 TGCGCA TGC^GCA
929 TGCGCA
KpnI 1190 GGTACC GGTAC^C
4668 GGTACC
MamI 1410 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1497 GATGAACATC
NcoI 2070 CCATGG C^CATGG
3962 CCATGG
PflMI 2551 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2835 CCAGAGAGTGG
RcaI 4689 TCATGA T^CATGA
4927 TCATGA
StuI 3802 AGGCCT AGG^CCT
4327 AGGCCT
Van91I 2551 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2835 CCAGAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BsrBI BsrDI BsrFI BsrI
BssHII BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NsiI NspI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI