################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII Acc65I AcyI AflII AgeI AhdI ApaI AscI Asp718I AspEI AviII BbeI BbrPI BclI BfrI BlpI Bpu1102I BsaAI BsaBI BsaHI BsiEI BsiWI BspDI BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacI SacII SapI SbfI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SspI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccIII 219 TCCGGA T^CCGGA Aor51HI 145 AGCGCT AGC^GCT AspI 5582 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3755 CCTAGG C^CTAGG BanI 1808 GGTGCC G^G[CT][GA]CC BbvCI 2626 CCTCAGC CCTCAGC(-5/-2) BcgI 2180 CGATGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 3054 AGATCT A^GATCT BlnI 3755 CCTAGG C^CTAGG BsaI 5403 GAGACC GGTCTC(1/5) BsaWI 219 TCCGGA [AT]^CCGG[AT] BseAI 219 TCCGGA T^CCGGA BsmBI 5433 CGTCTC CGTCTC(1/5) Bsp1407I 481 TGTACA T^GTACA BspEI 219 TCCGGA T^CCGGA BspLU11I 478 ACATGT A^CATGT BsrGI 481 TGTACA T^GTACA BssHII 135 GCGCGC G^CGCGC BstAPI 3023 GCACGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 1486 GACAGATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1409 GAAGAG CTCTTC(1/4) Eco47III 145 AGCGCT AGC^GCT HgaI 1847 GCGTC GACGC(5/10) Ksp632I 1409 GAAGAG CTCTTC(1/4) MroI 219 TCCGGA T^CCGGA PflFI 5582 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3175 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SalI 491 GTCGAC G^TCGAC ScaI 2673 AGTACT AGT^ACT SpeI 4834 ACTAGT A^CTAGT SspBI 481 TGTACA T^GTACA SwaI 3559 ATTTAAAT ATTT^AAAT Tth111I 5582 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3175 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1196 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflIII 478 ACATGT A^C[GA][CT]GT 4956 ACACGT AocI 971 CCTGAGG CC^T[ACGT]AGG 1646 CCTAAGG AseI 1221 ATTAAT AT^TAAT 2966 ATTAAT AsnI 1221 ATTAAT AT^TAAT 2966 ATTAAT BalI 5329 TGGCCA TGG^CCA 5413 TGGCCA BamHI 216 GGATCC G^GATCC 3427 GGATCC BciVI 576 GTATCC GTATCC(6/5) 5323 GTATCC BsgI 3030 GTGCAG GTGCAG(16/14) 3706 GTGCAG BsrFI 157 GCCGGC [GA]^CCGG[CT] 1781 GCCGGC Bsu36I 971 CCTGAGG CC^T[ACGT]AGG 1646 CCTAAGG Cfr10I 157 GCCGGC [GA]^CCGG[CT] 1781 GCCGGC EaeI 5329 TGGCCA [CT]^GGCC[GA] 5413 TGGCCA Eco81I 971 CCTGAGG CC^T[ACGT]AGG 1646 CCTAAGG EcoRI 734 GAATTC G^AATTC 3350 GAATTC HincII 491 GTCGAC GT[CT]^[GA]AC 2877 GTTGAC HindII 491 GTCGAC GT[CT]^[GA]AC 2877 GTTGAC MluNI 5329 TGGCCA TGG^CCA 5413 TGGCCA MscI 5329 TGGCCA TGG^CCA 5413 TGGCCA NaeI 157 GCCGGC GCC^GGC 1781 GCCGGC NgoMIV 157 GCCGGC G^CCGGC 1781 GCCGGC PshBI 1221 ATTAAT AT^TAAT 2966 ATTAAT PstI 1455 CTGCAG CTGCA^G 1523 CTGCAG SexAI 1366 ACCTGGT A^CC[AT]GGT 3587 ACCTGGT SmaI 212 CCCGGG CCC^GGG 231 CCCGGG SphI 2416 GCATGC GCATG^C 2684 GCATGC XcmI 580 CCAGATGCCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1102 CCATGTGTTTGGTGG XmaCI 212 CCCGGG C^CCGGG 231 CCCGGG XmaI 212 CCCGGG C^CCGGG 231 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AccII AciI AclI AcsI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI Psp1406I PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI