##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AcyI AflII AgeI
AhdI ApaI AscI Asp718I AspEI
AviII BbeI BbrPI BclI BfrI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsiEI BsiWI BspDI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NarI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacI SacII SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SspI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 219 TCCGGA T^CCGGA
Aor51HI 145 AGCGCT AGC^GCT
AspI 5582 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3755 CCTAGG C^CTAGG
BanI 1808 GGTGCC G^G[CT][GA]CC
BbvCI 2626 CCTCAGC CCTCAGC(-5/-2)
BcgI 2180 CGATGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3054 AGATCT A^GATCT
BlnI 3755 CCTAGG C^CTAGG
BsaI 5403 GAGACC GGTCTC(1/5)
BsaWI 219 TCCGGA [AT]^CCGG[AT]
BseAI 219 TCCGGA T^CCGGA
BsmBI 5433 CGTCTC CGTCTC(1/5)
Bsp1407I 481 TGTACA T^GTACA
BspEI 219 TCCGGA T^CCGGA
BspLU11I 478 ACATGT A^CATGT
BsrGI 481 TGTACA T^GTACA
BssHII 135 GCGCGC G^CGCGC
BstAPI 3023 GCACGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 1486 GACAGATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1409 GAAGAG CTCTTC(1/4)
Eco47III 145 AGCGCT AGC^GCT
HgaI 1847 GCGTC GACGC(5/10)
Ksp632I 1409 GAAGAG CTCTTC(1/4)
MroI 219 TCCGGA T^CCGGA
PflFI 5582 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3175 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 491 GTCGAC G^TCGAC
ScaI 2673 AGTACT AGT^ACT
SpeI 4834 ACTAGT A^CTAGT
SspBI 481 TGTACA T^GTACA
SwaI 3559 ATTTAAAT ATTT^AAAT
Tth111I 5582 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3175 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1196 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 478 ACATGT A^C[GA][CT]GT
4956 ACACGT
AocI 971 CCTGAGG CC^T[ACGT]AGG
1646 CCTAAGG
AseI 1221 ATTAAT AT^TAAT
2966 ATTAAT
AsnI 1221 ATTAAT AT^TAAT
2966 ATTAAT
BalI 5329 TGGCCA TGG^CCA
5413 TGGCCA
BamHI 216 GGATCC G^GATCC
3427 GGATCC
BciVI 576 GTATCC GTATCC(6/5)
5323 GTATCC
BsgI 3030 GTGCAG GTGCAG(16/14)
3706 GTGCAG
BsrFI 157 GCCGGC [GA]^CCGG[CT]
1781 GCCGGC
Bsu36I 971 CCTGAGG CC^T[ACGT]AGG
1646 CCTAAGG
Cfr10I 157 GCCGGC [GA]^CCGG[CT]
1781 GCCGGC
EaeI 5329 TGGCCA [CT]^GGCC[GA]
5413 TGGCCA
Eco81I 971 CCTGAGG CC^T[ACGT]AGG
1646 CCTAAGG
EcoRI 734 GAATTC G^AATTC
3350 GAATTC
HincII 491 GTCGAC GT[CT]^[GA]AC
2877 GTTGAC
HindII 491 GTCGAC GT[CT]^[GA]AC
2877 GTTGAC
MluNI 5329 TGGCCA TGG^CCA
5413 TGGCCA
MscI 5329 TGGCCA TGG^CCA
5413 TGGCCA
NaeI 157 GCCGGC GCC^GGC
1781 GCCGGC
NgoMIV 157 GCCGGC G^CCGGC
1781 GCCGGC
PshBI 1221 ATTAAT AT^TAAT
2966 ATTAAT
PstI 1455 CTGCAG CTGCA^G
1523 CTGCAG
SexAI 1366 ACCTGGT A^CC[AT]GGT
3587 ACCTGGT
SmaI 212 CCCGGG CCC^GGG
231 CCCGGG
SphI 2416 GCATGC GCATG^C
2684 GCATGC
XcmI 580 CCAGATGCCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1102 CCATGTGTTTGGTGG
XmaCI 212 CCCGGG C^CCGGG
231 CCCGGG
XmaI 212 CCCGGG C^CCGGG
231 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AclI AcsI
AfaI AluI Alw44I AlwI AlwNI
ApaLI ApoI Asp700I AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PleI PpuMI
Psp1406I PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI