##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AscI AvrII BciVI BfrI BlnI
BsaBI BseAI BsiWI BsmBI BspDI
BspEI BspLU11I BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraI
EcoO65I EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KspI MamI
MfeI MluI MroI MunI NheI
NotI NruI NsiI PacI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI ScaI SexAI SgrAI SnaBI
SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 4499 GACGTC GACGT^C
Acc65I 510 GGTACC G^GTACC
AcsI 2798 GAATTC [GA]^AATT[CT]
AhdI 395 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4436 GTGCAC G^TGCAC
AocI 356 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2139 AGCGCT AGC^GCT
ApaLI 4436 GTGCAC G^TGCAC
ApoI 2798 GAATTC [GA]^AATT[CT]
AseI 2099 ATTAAT AT^TAAT
AsnI 2099 ATTAAT AT^TAAT
Asp718I 510 GGTACC G^GTACC
AspEI 395 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4619 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 4811 GCTGAGG CCTCAGC(-5/-2)
BclI 5091 TGATCA T^GATCA
BglII 681 AGATCT A^GATCT
BlpI 1756 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1756 GCTCAGC GC^T[ACGT]AGC
BsaWI 1782 ACCGGA [AT]^CCGG[AT]
BsiEI 1469 CGGCCG CG[GA][CT]^CG
Bsp1407I 612 TGTACA T^GTACA
BsrGI 612 TGTACA T^GTACA
BstBI 2795 TTCGAA TT^CGAA
Bsu36I 356 CCTGAGG CC^T[ACGT]AGG
CelII 1756 GCTCAGC GC^T[ACGT]AGC
DrdI 2037 GACTAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1469 CGGCCG C^GGCCG
Eam1105I 395 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1469 CGGCCG C^GGCCG
Eco47III 2139 AGCGCT AGC^GCT
Eco52I 1469 CGGCCG C^GGCCG
Eco81I 356 CCTGAGG CC^T[ACGT]AGG
EcoRI 2798 GAATTC G^AATTC
FbaI 5091 TGATCA T^GATCA
KpnI 510 GGTACC GGTAC^C
NaeI 4690 GCCGGC GCC^GGC
NdeI 879 CATATG CA^TATG
NgoMIV 4690 GCCGGC G^CCGGC
NspV 2795 TTCGAA TT^CGAA
PflFI 4619 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2099 ATTAAT AT^TAAT
SbfI 2320 CCTGCAGG CCTGCA^GG
SfiI 2288 GGCCAGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2795 TTCGAA TT^CGAA
SphI 3420 GCATGC GCATG^C
Sse8387I 2320 CCTGCAGG CCTGCA^GG
SspBI 612 TGTACA T^GTACA
Tth111I 4619 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 2162 ACACGT A^C[GA][CT]GT
2643 ACGTGT
ApaI 1182 GGGCCC GGGCC^C
4595 GGGCCC
Asp700I 916 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4048 GAATGGGTTC
BamHI 28 GGATCC G^GATCC
3204 GGATCC
BbeI 3652 GGCGCC GGCGC^C
4402 GGCGCC
BbrPI 2452 CACGTG CAC^GTG
2642 CACGTG
BcgI 5048 CGAGCCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4485 GCATGGCCATCG
BfaI 899 CTAG C^TAG
1826 CTAG
BsmI 1239 GCATTC GAATGC(1/-1)
1335 GCATTC
BspHI 1590 TCATGA T^CATGA
5352 TCATGA
BsrFI 4672 GCCGGT [GA]^CCGG[CT]
4690 GCCGGC
BssSI 3863 CACGAG CACGAG(-5/-1)
1488 CTCGTG
Cfr10I 4672 GCCGGT [GA]^CCGG[CT]
4690 GCCGGC
KasI 3652 GGCGCC G^GCGCC
4402 GGCGCC
MaeI 899 CTAG C^TAG
1826 CTAG
NarI 3652 GGCGCC GG^CGCC
4402 GGCGCC
PmaCI 2452 CACGTG CAC^GTG
2642 CACGTG
PmlI 2452 CACGTG CAC^GTG
2642 CACGTG
PshAI 1618 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3395 GACCCCTGTC
RcaI 1590 TCATGA T^CATGA
5352 TCATGA
SacI 1106 GAGCTC GAGCT^C
1754 GAGCTC
SfoI 3652 GGCGCC GGC^GCC
4402 GGCGCC
SmaI 1448 CCCGGG CCC^GGG
4635 CCCGGG
SspI 2419 AATATT AAT^ATT
5085 AATATT
StuI 1200 AGGCCT AGG^CCT
2491 AGGCCT
XmaCI 1448 CCCGGG C^CCGGG
4635 CCCGGG
XmaI 1448 CCCGGG C^CCGGG
4635 CCCGGG
XmnI 916 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4048 GAATGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
AviII BalI BanI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII