################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AatII AgeI Alw44I ApaLI AscI Asp700I AviII BclI BsaBI BsiWI Bsp1407I BspLU11I BsrGI BssHII Bst1107I BstEII BstPI BstZ17I CpoI DraI DraIII DrdI EcoO65I EcoT22I FbaI FspI HpaI MamI MluI NdeI NheI NotI NruI NsiI PaeR7I PinAI PmeI PvuI RsrII SalI ScaI SfiI SgrAI SpeI SspBI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 5279 GGTACC G^GTACC AccIII 1179 TCCGGA T^CCGGA AclI 1408 AACGTT AA^CGTT AflII 2946 CTTAAG C^TTAAG AflIII 2774 ACACGT A^C[GA][CT]GT AhdI 1654 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 397 AGCGCT AGC^GCT AseI 2661 ATTAAT AT^TAAT AsnI 2661 ATTAAT AT^TAAT Asp718I 5279 GGTACC G^GTACC AspEI 1654 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BfrI 2946 CTTAAG C^TTAAG BglI 2575 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4544 AGATCT A^GATCT BlpI 3111 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3111 GCTCAGC GC^T[ACGT]AGC BsaWI 1179 TCCGGA [AT]^CCGG[AT] BseAI 1179 TCCGGA T^CCGGA BspDI 1237 ATCGAT AT^CGAT BspEI 1179 TCCGGA T^CCGGA BspHI 2962 TCATGA T^CATGA BsrBI 805 CCGCTC CCGCTC(-3/-3) BstBI 1957 TTCGAA TT^CGAA CelII 3111 GCTCAGC GC^T[ACGT]AGC ClaI 1237 ATCGAT AT^CGAT EagI 372 CGGCCG C^GGCCG Eam1105I 1654 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 372 CGGCCG C^GGCCG Eco47III 397 AGCGCT AGC^GCT Eco52I 372 CGGCCG C^GGCCG FseI 5698 GGCCGGCC GGCCGG^CC HincII 3200 GTCAAC GT[CT]^[GA]AC HindII 3200 GTCAAC GT[CT]^[GA]AC KpnI 5279 GGTACC GGTAC^C KspI 578 CCGCGG CCGC^GG MfeI 1437 CAATTG C^AATTG MroI 1179 TCCGGA T^CCGGA MunI 1437 CAATTG C^AATTG NcoI 1057 CCATGG C^CATGG NspV 1957 TTCGAA TT^CGAA PacI 4624 TTAATTAA TTAAT^TAA PshBI 2661 ATTAAT AT^TAAT Psp1406I 1408 AACGTT AA^CGTT RcaI 2962 TCATGA T^CATGA SacII 578 CCGCGG CCGC^GG SbfI 5390 CCTGCAGG CCTGCA^GG SfuI 1957 TTCGAA TT^CGAA SnaBI 665 TACGTA TAC^GTA SphI 5121 GCATGC GCATG^C Sse8387I 5390 CCTGCAGG CCTGCA^GG XbaI 4568 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccI 668 GTAGAC GT^[AC][GT]AC 3489 GTCTAC AocI 2137 CCTGAGG CC^T[ACGT]AGG 3179 CCTGAGG BcgI 1882 GCACGTGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4373 GCAGGAGAATCG BciVI 183 GGATAC GTATCC(6/5) 1152 GGATAC BsiEI 62 CGACCG CG[GA][CT]^CG 372 CGGCCG BsmI 2890 GCATTC GAATGC(1/-1) 4432 GCATTC Bsu36I 2137 CCTGAGG CC^T[ACGT]AGG 3179 CCTGAGG Eco81I 2137 CCTGAGG CC^T[ACGT]AGG 3179 CCTGAGG EcoRI 976 GAATTC G^AATTC 1306 GAATTC EcoRV 3581 GATATC GAT^ATC 5480 GATATC NspI 3045 ACATGC [GA]CATG^[CT] 5121 GCATGC PflMI 313 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5004 CCAGGATCTGG PshAI 226 GACACTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5349 GACCTGGGTC SexAI 4922 ACCAGGT A^CC[AT]GGT 5298 ACCTGGT Van91I 313 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5004 CCAGGATCTGG XcmI 1048 CCAATGGATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5289 CCAGCAGAGACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AspI AvaI AvaII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcnI BfaI BlnI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PflFI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII XmaCI XmaI