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# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AgeI Alw44I ApaLI AscI
Asp700I AviII BclI BsaBI BsiWI
Bsp1407I BspLU11I BsrGI BssHII Bst1107I
BstEII BstPI BstZ17I CpoI DraI
DraIII DrdI EcoO65I EcoT22I FbaI
FspI HpaI MamI MluI NdeI
NheI NotI NruI NsiI PaeR7I
PinAI PmeI PvuI RsrII SalI
ScaI SfiI SgrAI SpeI SspBI
SspI SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 5279 GGTACC G^GTACC
AccIII 1179 TCCGGA T^CCGGA
AclI 1408 AACGTT AA^CGTT
AflII 2946 CTTAAG C^TTAAG
AflIII 2774 ACACGT A^C[GA][CT]GT
AhdI 1654 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 397 AGCGCT AGC^GCT
AseI 2661 ATTAAT AT^TAAT
AsnI 2661 ATTAAT AT^TAAT
Asp718I 5279 GGTACC G^GTACC
AspEI 1654 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfrI 2946 CTTAAG C^TTAAG
BglI 2575 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4544 AGATCT A^GATCT
BlpI 3111 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3111 GCTCAGC GC^T[ACGT]AGC
BsaWI 1179 TCCGGA [AT]^CCGG[AT]
BseAI 1179 TCCGGA T^CCGGA
BspDI 1237 ATCGAT AT^CGAT
BspEI 1179 TCCGGA T^CCGGA
BspHI 2962 TCATGA T^CATGA
BsrBI 805 CCGCTC CCGCTC(-3/-3)
BstBI 1957 TTCGAA TT^CGAA
CelII 3111 GCTCAGC GC^T[ACGT]AGC
ClaI 1237 ATCGAT AT^CGAT
EagI 372 CGGCCG C^GGCCG
Eam1105I 1654 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 372 CGGCCG C^GGCCG
Eco47III 397 AGCGCT AGC^GCT
Eco52I 372 CGGCCG C^GGCCG
FseI 5698 GGCCGGCC GGCCGG^CC
HincII 3200 GTCAAC GT[CT]^[GA]AC
HindII 3200 GTCAAC GT[CT]^[GA]AC
KpnI 5279 GGTACC GGTAC^C
KspI 578 CCGCGG CCGC^GG
MfeI 1437 CAATTG C^AATTG
MroI 1179 TCCGGA T^CCGGA
MunI 1437 CAATTG C^AATTG
NcoI 1057 CCATGG C^CATGG
NspV 1957 TTCGAA TT^CGAA
PacI 4624 TTAATTAA TTAAT^TAA
PshBI 2661 ATTAAT AT^TAAT
Psp1406I 1408 AACGTT AA^CGTT
RcaI 2962 TCATGA T^CATGA
SacII 578 CCGCGG CCGC^GG
SbfI 5390 CCTGCAGG CCTGCA^GG
SfuI 1957 TTCGAA TT^CGAA
SnaBI 665 TACGTA TAC^GTA
SphI 5121 GCATGC GCATG^C
Sse8387I 5390 CCTGCAGG CCTGCA^GG
XbaI 4568 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 668 GTAGAC GT^[AC][GT]AC
3489 GTCTAC
AocI 2137 CCTGAGG CC^T[ACGT]AGG
3179 CCTGAGG
BcgI 1882 GCACGTGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4373 GCAGGAGAATCG
BciVI 183 GGATAC GTATCC(6/5)
1152 GGATAC
BsiEI 62 CGACCG CG[GA][CT]^CG
372 CGGCCG
BsmI 2890 GCATTC GAATGC(1/-1)
4432 GCATTC
Bsu36I 2137 CCTGAGG CC^T[ACGT]AGG
3179 CCTGAGG
Eco81I 2137 CCTGAGG CC^T[ACGT]AGG
3179 CCTGAGG
EcoRI 976 GAATTC G^AATTC
1306 GAATTC
EcoRV 3581 GATATC GAT^ATC
5480 GATATC
NspI 3045 ACATGC [GA]CATG^[CT]
5121 GCATGC
PflMI 313 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5004 CCAGGATCTGG
PshAI 226 GACACTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5349 GACCTGGGTC
SexAI 4922 ACCAGGT A^CC[AT]GGT
5298 ACCTGGT
Van91I 313 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5004 CCAGGATCTGG
XcmI 1048 CCAATGGATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5289 CCAGCAGAGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AspI AvaI AvaII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcnI
BfaI BlnI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PflFI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII
XmaCI XmaI