################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI AocI ApaI AscI Asp718I AspEI AviII BamHI BanI BbeI BbvCI BcgI BglI BsaHI BseAI BsiEI BsiWI BspDI BspEI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV FseI FspI Hin1I KasI KpnI KspI MluI MroI MspA1I MvnI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmeI PpuMI Psp1406I PstI PvuI PvuII RsrII SacII SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 1775 GTCGAC GT^[AC][GT]AC AlwNI 2168 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 9 CCCGAG C^[CT]CG[GA]G BbrPI 481 CACGTG CAC^GTG BciVI 618 GGATAC GTATCC(6/5) BcnI 4961 CCGGG CC^[GC]GG BglII 4633 AGATCT A^GATCT BlpI 4942 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4942 GCTGAGC GC^T[ACGT]AGC BsaAI 481 CACGTG [CT]AC^GT[GA] BsaWI 1556 ACCGGA [AT]^CCGG[AT] BsgI 2851 CTGCAC GTGCAG(16/14) BsmI 1184 GAATGC GAATGC(1/-1) BsoBI 9 CCCGAG C^[CT]CG[GA]G Bsp1407I 2098 TGTACA T^GTACA BsrGI 2098 TGTACA T^GTACA BstAPI 4743 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 489 TTCGAA TT^CGAA BstXI 2634 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 4942 GCTGAGC GC^T[ACGT]AGC DraII 1663 AGGCCCC [GA]G^G[ACGT]CC[CT] DrdI 5122 GACACCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 1663 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoT22I 4411 ATGCAT ATGCA^T HgaI 3970 GCGTC GACGC(5/10) MfeI 738 CAATTG C^AATTG MunI 738 CAATTG C^AATTG NciI 4961 CCGGG CC^[GC]GG NsiI 4411 ATGCAT ATGCA^T NspV 489 TTCGAA TT^CGAA PmaCI 481 CACGTG CAC^GTG PmlI 481 CACGTG CAC^GTG PshAI 5146 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 1775 GTCGAC G^TCGAC SfcI 2243 CTGTAG C^T[GA][CT]AG SfuI 489 TTCGAA TT^CGAA SpeI 803 ACTAGT A^CTAGT SspBI 2098 TGTACA T^GTACA StuI 4767 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Aor51HI 104 AGCGCT AGC^GCT 348 AGCGCT AspI 2333 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3966 GACAGCGTC AvaII 4654 GGTCC G^G[AT]CC 4709 GGACC AvrII 1258 CCTAGG C^CTAGG 2302 CCTAGG BlnI 1258 CCTAGG C^CTAGG 2302 CCTAGG BsaBI 848 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5515 GATGATAATC BseRI 2788 CTCCTC GAGGAG(10/8) 3779 GAGGAG BsmBI 2714 CGTCTC CGTCTC(1/5) 242 GAGACG BspMI 1973 ACCTGC ACCTGC(4/8) 5223 ACCTGC DsaI 1714 CCATGG C^C[GA][CT]GG 4875 CCATGG Eco47III 104 AGCGCT AGC^GCT 348 AGCGCT EcoNI 68 CCTGTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 608 CCTTTAGTAGG EcoRI 1337 GAATTC G^AATTC 2906 GAATTC FauI 1019 CCCGC CCCGC(4/6) 5458 GCGGG HapII 1557 CCGG C^CGG 4961 CCGG HpaII 1557 CCGG C^CGG 4961 CCGG MamI 848 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5515 GATGATAATC MspI 1557 CCGG C^CGG 4961 CCGG NcoI 1714 CCATGG C^CATGG 4875 CCATGG NdeI 1190 CATATG CA^TATG 1353 CATATG PflFI 2333 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3966 GACAGCGTC SacI 12 GAGCTC GAGCT^C 954 GAGCTC SapI 2349 GAAGAGC GCTCTTC(1/4) 5299 GAAGAGC ScaI 1869 AGTACT AGT^ACT 5217 AGTACT SphI 1080 GCATGC GCATG^C 2033 GCATGC Tth111I 2333 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3966 GACAGCGTC XbaI 3432 TCTAGA T^CTAGA 4630 TCTAGA XcmI 39 CCAAGAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 306 CCAACATAGCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcsI AfaI AflII AflIII AluI Alw44I AlwI ApaLI ApoI AseI AsnI Asp700I AspHI BalI BanII BbsI BbvI BclI BfaI BfrI BmyI BpmI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoRII EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HincII HindII HindIII HinfI HpaI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI