##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AocI ApaI
AscI Asp718I AspEI AviII BamHI
BanI BbeI BbvCI BcgI BglI
BsaHI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRV FseI FspI
Hin1I KasI KpnI KspI MluI
MroI MspA1I MvnI NaeI NarI
NgoMIV NheI NotI NruI PacI
PaeR7I PflMI PinAI PmeI PpuMI
Psp1406I PstI PvuI PvuII RsrII
SacII SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SwaI
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1775 GTCGAC GT^[AC][GT]AC
AlwNI 2168 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 9 CCCGAG C^[CT]CG[GA]G
BbrPI 481 CACGTG CAC^GTG
BciVI 618 GGATAC GTATCC(6/5)
BcnI 4961 CCGGG CC^[GC]GG
BglII 4633 AGATCT A^GATCT
BlpI 4942 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4942 GCTGAGC GC^T[ACGT]AGC
BsaAI 481 CACGTG [CT]AC^GT[GA]
BsaWI 1556 ACCGGA [AT]^CCGG[AT]
BsgI 2851 CTGCAC GTGCAG(16/14)
BsmI 1184 GAATGC GAATGC(1/-1)
BsoBI 9 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2098 TGTACA T^GTACA
BsrGI 2098 TGTACA T^GTACA
BstAPI 4743 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 489 TTCGAA TT^CGAA
BstXI 2634 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4942 GCTGAGC GC^T[ACGT]AGC
DraII 1663 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 5122 GACACCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1663 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 4411 ATGCAT ATGCA^T
HgaI 3970 GCGTC GACGC(5/10)
MfeI 738 CAATTG C^AATTG
MunI 738 CAATTG C^AATTG
NciI 4961 CCGGG CC^[GC]GG
NsiI 4411 ATGCAT ATGCA^T
NspV 489 TTCGAA TT^CGAA
PmaCI 481 CACGTG CAC^GTG
PmlI 481 CACGTG CAC^GTG
PshAI 5146 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1775 GTCGAC G^TCGAC
SfcI 2243 CTGTAG C^T[GA][CT]AG
SfuI 489 TTCGAA TT^CGAA
SpeI 803 ACTAGT A^CTAGT
SspBI 2098 TGTACA T^GTACA
StuI 4767 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Aor51HI 104 AGCGCT AGC^GCT
348 AGCGCT
AspI 2333 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3966 GACAGCGTC
AvaII 4654 GGTCC G^G[AT]CC
4709 GGACC
AvrII 1258 CCTAGG C^CTAGG
2302 CCTAGG
BlnI 1258 CCTAGG C^CTAGG
2302 CCTAGG
BsaBI 848 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5515 GATGATAATC
BseRI 2788 CTCCTC GAGGAG(10/8)
3779 GAGGAG
BsmBI 2714 CGTCTC CGTCTC(1/5)
242 GAGACG
BspMI 1973 ACCTGC ACCTGC(4/8)
5223 ACCTGC
DsaI 1714 CCATGG C^C[GA][CT]GG
4875 CCATGG
Eco47III 104 AGCGCT AGC^GCT
348 AGCGCT
EcoNI 68 CCTGTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
608 CCTTTAGTAGG
EcoRI 1337 GAATTC G^AATTC
2906 GAATTC
FauI 1019 CCCGC CCCGC(4/6)
5458 GCGGG
HapII 1557 CCGG C^CGG
4961 CCGG
HpaII 1557 CCGG C^CGG
4961 CCGG
MamI 848 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5515 GATGATAATC
MspI 1557 CCGG C^CGG
4961 CCGG
NcoI 1714 CCATGG C^CATGG
4875 CCATGG
NdeI 1190 CATATG CA^TATG
1353 CATATG
PflFI 2333 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3966 GACAGCGTC
SacI 12 GAGCTC GAGCT^C
954 GAGCTC
SapI 2349 GAAGAGC GCTCTTC(1/4)
5299 GAAGAGC
ScaI 1869 AGTACT AGT^ACT
5217 AGTACT
SphI 1080 GCATGC GCATG^C
2033 GCATGC
Tth111I 2333 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3966 GACAGCGTC
XbaI 3432 TCTAGA T^CTAGA
4630 TCTAGA
XcmI 39 CCAAGAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
306 CCAACATAGCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI Alw44I AlwI ApaLI ApoI
AseI AsnI Asp700I AspHI BalI
BanII BbsI BbvI BclI BfaI
BfrI BmyI BpmI BpuAI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspLU11I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HincII HindII HindIII HinfI
HpaI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI