################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AccII AclI AflII AgeI ApaI AscI AspI AviII AvrII BbeI BbrPI BfrI BlnI BsaAI BsiEI BsiWI BspDI BsrBI BssHII BssSI Bst1107I BstUI BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I EcoRV FseI FspI HpaI KasI KspI MluI MvnI NarI NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 4376 GACGTC GACGT^C AccI 105 GTCGAC GT^[AC][GT]AC AccIII 54 TCCGGA T^CCGGA AflIII 1386 ACATGT A^C[GA][CT]GT AhdI 2023 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 400 GTGCAC G^TGCAC AocI 1722 CCTGAGG CC^T[ACGT]AGG Aor51HI 22 AGCGCT AGC^GCT ApaLI 400 GTGCAC G^TGCAC AseI 322 ATTAAT AT^TAAT AsnI 322 ATTAAT AT^TAAT AspEI 2023 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BcgI 402 GCACAGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1512 TGATCA T^GATCA BglI 1579 GCCTCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 987 GCTGAGC GC^T[ACGT]AGC Bpu1102I 987 GCTGAGC GC^T[ACGT]AGC BsaBI 1710 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 54 TCCGGA [AT]^CCGG[AT] BseAI 54 TCCGGA T^CCGGA BsmI 153 GAATGC GAATGC(1/-1) BspEI 54 TCCGGA T^CCGGA BspLU11I 1386 ACATGT A^CATGT BsrDI 1911 CATTGC GCAATG(2/0) BstAPI 2137 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1722 CCTGAGG CC^T[ACGT]AGG CelII 987 GCTGAGC GC^T[ACGT]AGC Eam1105I 2023 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 22 AGCGCT AGC^GCT Eco81I 1722 CCTGAGG CC^T[ACGT]AGG FbaI 1512 TGATCA T^GATCA HaeII 22 AGCGCT [GA]GCGC^[CT] MamI 1710 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 54 TCCGGA T^CCGGA NaeI 2253 GCCGGC GCC^GGC NgoMIV 2253 GCCGGC G^CCGGC PshAI 186 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 322 ATTAAT AT^TAAT SalI 105 GTCGAC G^TCGAC SapI 1749 GAAGAGC GCTCTTC(1/4) SexAI 2282 ACCTGGT A^CC[AT]GGT SpeI 1923 ACTAGT A^CTAGT SphI 2273 GCATGC GCATG^C SspI 3163 AATATT AAT^ATT StuI 3216 AGGCCT AGG^CCT XbaI 1369 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AcyI 407 GGCGTC G[GA]^CG[CT]C 4376 GACGTC AvaI 195 CTCGGG C^[CT]CG[GA]G 2301 CTCGGG BalI 2715 TGGCCA TGG^CCA 4917 TGGCCA BamHI 959 GGATCC G^GATCC 1814 GGATCC BbsI 3195 GAAGAC GAAGAC(2/6) 5070 GAAGAC BciVI 2945 GGATAC GTATCC(6/5) 3880 GGATAC BpuAI 3195 GAAGAC GAAGAC(2/6) 5070 GAAGAC BsaHI 407 GGCGTC G[GA]^CG[CT]C 4376 GACGTC BsmBI 192 CGTCTC CGTCTC(1/5) 4378 CGTCTC BsoBI 195 CTCGGG C^[CT]CG[GA]G 2301 CTCGGG BspHI 758 TCATGA T^CATGA 1539 TCATGA BspMI 532 ACCTGC ACCTGC(4/8) 4211 ACCTGC BsrFI 2253 GCCGGC [GA]^CCGG[CT] 2452 ACCGGC BstBI 1313 TTCGAA TT^CGAA 1525 TTCGAA CfoI 23 GCGC GCG^C 889 GCGC Cfr10I 2253 GCCGGC [GA]^CCGG[CT] 2452 ACCGGC DraIII 394 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 2188 CACTTTGTG EcoRI 923 GAATTC G^AATTC 2833 GAATTC EcoT22I 3100 ATGCAT ATGCA^T 5124 ATGCAT HgaI 408 GCGTC GACGC(5/10) 1536 GCGTC HhaI 23 GCGC GCG^C 889 GCGC Hin1I 407 GGCGTC G[GA]^CG[CT]C 4376 GACGTC HinP1I 23 GCGC G^CGC 889 GCGC HindIII 672 AAGCTT A^AGCTT 1883 AAGCTT MfeI 2801 CAATTG C^AATTG 5065 CAATTG MluNI 2715 TGGCCA TGG^CCA 4917 TGGCCA MscI 2715 TGGCCA TGG^CCA 4917 TGGCCA MunI 2801 CAATTG C^AATTG 5065 CAATTG NcoI 1574 CCATGG C^CATGG 1608 CCATGG NsiI 3100 ATGCAT ATGCA^T 5124 ATGCAT NspV 1313 TTCGAA TT^CGAA 1525 TTCGAA PvuII 492 CAGCTG CAG^CTG 985 CAGCTG RcaI 758 TCATGA T^CATGA 1539 TCATGA SfuI 1313 TTCGAA TT^CGAA 1525 TTCGAA SmlI 360 CTCAAG C^T[CT][GA]AG 2639 CTCAAG XcmI 1574 CCATGGCCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4216 CCACGGATCGAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### Acc65I AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I Asp718I AspHI AvaII BanI BanII BbvCI BbvI BcnI BfaI BglII BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI