##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccII AclI AflII AgeI ApaI
AscI AspI AviII AvrII BbeI
BbrPI BfrI BlnI BsaAI BsiEI
BsiWI BspDI BsrBI BssHII BssSI
Bst1107I BstUI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco52I EcoRV
FseI FspI HpaI KasI KspI
MluI MvnI NarI NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 4376 GACGTC GACGT^C
AccI 105 GTCGAC GT^[AC][GT]AC
AccIII 54 TCCGGA T^CCGGA
AflIII 1386 ACATGT A^C[GA][CT]GT
AhdI 2023 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 400 GTGCAC G^TGCAC
AocI 1722 CCTGAGG CC^T[ACGT]AGG
Aor51HI 22 AGCGCT AGC^GCT
ApaLI 400 GTGCAC G^TGCAC
AseI 322 ATTAAT AT^TAAT
AsnI 322 ATTAAT AT^TAAT
AspEI 2023 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 402 GCACAGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1512 TGATCA T^GATCA
BglI 1579 GCCTCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 987 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 987 GCTGAGC GC^T[ACGT]AGC
BsaBI 1710 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 54 TCCGGA [AT]^CCGG[AT]
BseAI 54 TCCGGA T^CCGGA
BsmI 153 GAATGC GAATGC(1/-1)
BspEI 54 TCCGGA T^CCGGA
BspLU11I 1386 ACATGT A^CATGT
BsrDI 1911 CATTGC GCAATG(2/0)
BstAPI 2137 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1722 CCTGAGG CC^T[ACGT]AGG
CelII 987 GCTGAGC GC^T[ACGT]AGC
Eam1105I 2023 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 22 AGCGCT AGC^GCT
Eco81I 1722 CCTGAGG CC^T[ACGT]AGG
FbaI 1512 TGATCA T^GATCA
HaeII 22 AGCGCT [GA]GCGC^[CT]
MamI 1710 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 54 TCCGGA T^CCGGA
NaeI 2253 GCCGGC GCC^GGC
NgoMIV 2253 GCCGGC G^CCGGC
PshAI 186 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 322 ATTAAT AT^TAAT
SalI 105 GTCGAC G^TCGAC
SapI 1749 GAAGAGC GCTCTTC(1/4)
SexAI 2282 ACCTGGT A^CC[AT]GGT
SpeI 1923 ACTAGT A^CTAGT
SphI 2273 GCATGC GCATG^C
SspI 3163 AATATT AAT^ATT
StuI 3216 AGGCCT AGG^CCT
XbaI 1369 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 407 GGCGTC G[GA]^CG[CT]C
4376 GACGTC
AvaI 195 CTCGGG C^[CT]CG[GA]G
2301 CTCGGG
BalI 2715 TGGCCA TGG^CCA
4917 TGGCCA
BamHI 959 GGATCC G^GATCC
1814 GGATCC
BbsI 3195 GAAGAC GAAGAC(2/6)
5070 GAAGAC
BciVI 2945 GGATAC GTATCC(6/5)
3880 GGATAC
BpuAI 3195 GAAGAC GAAGAC(2/6)
5070 GAAGAC
BsaHI 407 GGCGTC G[GA]^CG[CT]C
4376 GACGTC
BsmBI 192 CGTCTC CGTCTC(1/5)
4378 CGTCTC
BsoBI 195 CTCGGG C^[CT]CG[GA]G
2301 CTCGGG
BspHI 758 TCATGA T^CATGA
1539 TCATGA
BspMI 532 ACCTGC ACCTGC(4/8)
4211 ACCTGC
BsrFI 2253 GCCGGC [GA]^CCGG[CT]
2452 ACCGGC
BstBI 1313 TTCGAA TT^CGAA
1525 TTCGAA
CfoI 23 GCGC GCG^C
889 GCGC
Cfr10I 2253 GCCGGC [GA]^CCGG[CT]
2452 ACCGGC
DraIII 394 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2188 CACTTTGTG
EcoRI 923 GAATTC G^AATTC
2833 GAATTC
EcoT22I 3100 ATGCAT ATGCA^T
5124 ATGCAT
HgaI 408 GCGTC GACGC(5/10)
1536 GCGTC
HhaI 23 GCGC GCG^C
889 GCGC
Hin1I 407 GGCGTC G[GA]^CG[CT]C
4376 GACGTC
HinP1I 23 GCGC G^CGC
889 GCGC
HindIII 672 AAGCTT A^AGCTT
1883 AAGCTT
MfeI 2801 CAATTG C^AATTG
5065 CAATTG
MluNI 2715 TGGCCA TGG^CCA
4917 TGGCCA
MscI 2715 TGGCCA TGG^CCA
4917 TGGCCA
MunI 2801 CAATTG C^AATTG
5065 CAATTG
NcoI 1574 CCATGG C^CATGG
1608 CCATGG
NsiI 3100 ATGCAT ATGCA^T
5124 ATGCAT
NspV 1313 TTCGAA TT^CGAA
1525 TTCGAA
PvuII 492 CAGCTG CAG^CTG
985 CAGCTG
RcaI 758 TCATGA T^CATGA
1539 TCATGA
SfuI 1313 TTCGAA TT^CGAA
1525 TTCGAA
SmlI 360 CTCAAG C^T[CT][GA]AG
2639 CTCAAG
XcmI 1574 CCATGGCCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4216 CCACGGATCGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I Asp718I
AspHI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI