##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
AocI ApaI ApaLI AscI Asp718I
AvrII BbrPI BciVI BfrI BlnI
BsiWI BsmI BspDI BssSI BstBI
Bsu36I ClaI CpoI Eco81I EcoT22I
FseI KpnI MfeI MluI MunI
NcoI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SbfI
ScaI SfiI SfuI SnaBI SpeI
Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1236 GACGTC GACGT^C
AccIII 1681 TCCGGA T^CCGGA
AflIII 4193 ACATGT A^C[GA][CT]GT
AhdI 2996 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2816 AGCGCT AGC^GCT
AseI 797 ATTAAT AT^TAAT
AsnI 797 ATTAAT AT^TAAT
Asp700I 175 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2996 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1096 TGCGCA TGC^GCA
BglII 1285 AGATCT A^GATCT
BsaAI 1146 TACGTG [CT]AC^GT[GA]
BseAI 1681 TCCGGA T^CCGGA
BspEI 1681 TCCGGA T^CCGGA
BspLU11I 4193 ACATGT A^CATGT
Bst1107I 5018 GTATAC GTA^TAC
BstEII 1075 GGTCACC G^GT[ACGT]ACC
BstPI 1075 GGTCACC G^GT[ACGT]ACC
BstZ17I 5018 GTATAC GTA^TAC
DrdI 3963 GACGGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3037 CGGCCG C^GGCCG
Eam1105I 2996 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3037 CGGCCG C^GGCCG
Eco47III 2816 AGCGCT AGC^GCT
Eco52I 3037 CGGCCG C^GGCCG
EcoO65I 1075 GGTCACC G^GT[ACGT]ACC
EcoRI 5271 GAATTC G^AATTC
EcoRV 3995 GATATC GAT^ATC
FspI 1096 TGCGCA TGC^GCA
HpaI 5194 GTTAAC GTT^AAC
KspI 217 CCGCGG CCGC^GG
MroI 1681 TCCGGA T^CCGGA
NdeI 3689 CATATG CA^TATG
PaeR7I 554 CTCGAG C^TCGAG
PshAI 4466 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 797 ATTAAT AT^TAAT
SacII 217 CCGCGG CCGC^GG
SalI 4898 GTCGAC G^TCGAC
SexAI 1108 ACCAGGT A^CC[AT]GGT
SgrAI 2592 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 4186 CCCGGG CCC^GGG
StuI 1298 AGGCCT AGG^CCT
XhoI 554 CTCGAG C^TCGAG
XmaCI 4186 CCCGGG C^CCGGG
XmaI 4186 CCCGGG C^CCGGG
XmnI 175 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 4898 GTCGAC GT^[AC][GT]AC
5018 GTATAC
AspI 1233 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2576 GACATCGTC
BamHI 394 GGATCC G^GATCC
1995 GGATCC
BbeI 226 GGCGCC GGCGC^C
1813 GGCGCC
BcgI 1705 GCATGATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3265 GCAAGGTGATCG
BlpI 2819 GCTCAGC GC^T[ACGT]AGC
3426 GCTGAGC
Bpu1102I 2819 GCTCAGC GC^T[ACGT]AGC
3426 GCTGAGC
BsaBI 887 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2862 GATATTGATC
BsiEI 602 CGACCG CG[GA][CT]^CG
3037 CGGCCG
BsmBI 4356 CGTCTC CGTCTC(1/5)
1668 GAGACG
Bsp1407I 4211 TGTACA T^GTACA
5173 TGTACA
BsrGI 4211 TGTACA T^GTACA
5173 TGTACA
CelII 2819 GCTCAGC GC^T[ACGT]AGC
3426 GCTGAGC
DraI 2025 TTTAAA TTT^AAA
4379 TTTAAA
DraIII 3213 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3886 CACCCTGTG
DsaI 217 CCGCGG C^C[GA][CT]GG
847 CCGTGG
KasI 226 GGCGCC G^GCGCC
1813 GGCGCC
MamI 887 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2862 GATATTGATC
NarI 226 GGCGCC GG^CGCC
1813 GGCGCC
PflFI 1233 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2576 GACATCGTC
PflMI 230 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2123 CCAGTGTGTGG
SapI 42 GCTCTTC GCTCTTC(1/4)
533 GCTCTTC
SfoI 226 GGCGCC GGC^GCC
1813 GGCGCC
SmlI 554 CTCGAG C^T[CT][GA]AG
927 CTCAAG
SspBI 4211 TGTACA T^GTACA
5173 TGTACA
Tth111I 1233 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2576 GACATCGTC
Van91I 230 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2123 CCAGTGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII