##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI Asp718I AspEI AvrII BbeI
BbrPI BcgI BglI BglII BlnI
BsiWI Bst1107I BstZ17I CpoI DraIII
Eam1105I HpaI KasI KpnI MfeI
MluI MunI NarI NcoI NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SfiI SfoI SgrAI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 5823 TCCGGA T^CCGGA
AflII 5017 CTTAAG C^TTAAG
Aor51HI 3417 AGCGCT AGC^GCT
ApaI 3157 GGGCCC GGGCC^C
AviII 1236 TGCGCA TGC^GCA
BalI 1751 TGGCCA TGG^CCA
BamHI 3429 GGATCC G^GATCC
BciVI 824 GTATCC GTATCC(6/5)
BclI 2586 TGATCA T^GATCA
BfrI 5017 CTTAAG C^TTAAG
BsaBI 2587 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 5823 TCCGGA [AT]^CCGG[AT]
BseAI 5823 TCCGGA T^CCGGA
BsmBI 3543 CGTCTC CGTCTC(1/5)
BspDI 2758 ATCGAT AT^CGAT
BspEI 5823 TCCGGA T^CCGGA
BspHI 2555 TCATGA T^CATGA
BssHII 3714 GCGCGC G^CGCGC
ClaI 2758 ATCGAT AT^CGAT
Eco47III 3417 AGCGCT AGC^GCT
EcoRI 5090 GAATTC G^AATTC
EcoT22I 1934 ATGCAT ATGCA^T
FbaI 2586 TGATCA T^GATCA
FseI 3753 GGCCGGCC GGCCGG^CC
FspI 1236 TGCGCA TGC^GCA
HincII 4798 GTCAAC GT[CT]^[GA]AC
HindII 4798 GTCAAC GT[CT]^[GA]AC
KspI 2051 CCGCGG CCGC^GG
MamI 2587 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1751 TGGCCA TGG^CCA
MroI 5823 TCCGGA T^CCGGA
MscI 1751 TGGCCA TGG^CCA
NsiI 1934 ATGCAT ATGCA^T
PaeR7I 4811 CTCGAG C^TCGAG
RcaI 2555 TCATGA T^CATGA
SacII 2051 CCGCGG CCGC^GG
SbfI 1467 CCTGCAGG CCTGCA^GG
SmaI 3561 CCCGGG CCC^GGG
SnaBI 1290 TACGTA TAC^GTA
SphI 992 GCATGC GCATG^C
Sse8387I 1467 CCTGCAGG CCTGCA^GG
XbaI 6177 TCTAGA T^CTAGA
XcmI 1079 CCATGCTGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4811 CTCGAG C^TCGAG
XmaCI 3561 CCCGGG C^CCGGG
XmaI 3561 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 3541 GGCGTC G[GA]^CG[CT]C
4795 GGCGTC
AocI 356 CCTCAGG CC^T[ACGT]AGG
1954 CCTGAGG
AseI 2773 ATTAAT AT^TAAT
5973 ATTAAT
AsnI 2773 ATTAAT AT^TAAT
5973 ATTAAT
BanI 419 GGCACC G^G[CT][GA]CC
5111 GGTGCC
BbvCI 1096 CCTCAGC CCTCAGC(-5/-2)
2079 GCTGAGG
BlpI 2842 GCTCAGC GC^T[ACGT]AGC
4086 GCTGAGC
Bpu1102I 2842 GCTCAGC GC^T[ACGT]AGC
4086 GCTGAGC
BsaAI 1207 CACGTA [CT]AC^GT[GA]
1290 TACGTA
BsaHI 3541 GGCGTC G[GA]^CG[CT]C
4795 GGCGTC
BsaI 514 GGTCTC GGTCTC(1/5)
3182 GGTCTC
Bsp1407I 2742 TGTACA T^GTACA
5264 TGTACA
BspLU11I 4757 ACATGT A^CATGT
5124 ACATGT
BsrBI 3865 GAGCGG CCGCTC(-3/-3)
3949 GAGCGG
BsrGI 2742 TGTACA T^GTACA
5264 TGTACA
BstEII 2653 GGTGACC G^GT[ACGT]ACC
4060 GGTCACC
BstPI 2653 GGTGACC G^GT[ACGT]ACC
4060 GGTCACC
BstXI 107 CCAGCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3531 CCAGGTAGCTGG
Bsu36I 356 CCTCAGG CC^T[ACGT]AGG
1954 CCTGAGG
CelII 2842 GCTCAGC GC^T[ACGT]AGC
4086 GCTGAGC
DrdI 2902 GACAGCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3316 GACGCTAGTGTC
EagI 2005 CGGCCG C^GGCCG
3868 CGGCCG
EclXI 2005 CGGCCG C^GGCCG
3868 CGGCCG
Eco52I 2005 CGGCCG C^GGCCG
3868 CGGCCG
Eco81I 356 CCTCAGG CC^T[ACGT]AGG
1954 CCTGAGG
EcoO65I 2653 GGTGACC G^GT[ACGT]ACC
4060 GGTCACC
EcoRV 5406 GATATC GAT^ATC
6079 GATATC
Hin1I 3541 GGCGTC G[GA]^CG[CT]C
4795 GGCGTC
HindIII 1371 AAGCTT A^AGCTT
4245 AAGCTT
NaeI 3754 GCCGGC GCC^GGC
4129 GCCGGC
NgoMIV 3754 GCCGGC G^CCGGC
4129 GCCGGC
PshAI 508 GACTTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
835 GACCTTCGTC
PshBI 2773 ATTAAT AT^TAAT
5973 ATTAAT
ScaI 248 AGTACT AGT^ACT
5318 AGTACT
SpeI 3477 ACTAGT A^CTAGT
5321 ACTAGT
SspBI 2742 TGTACA T^GTACA
5264 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI Asp700I AspHI AspI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstBI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI NspV PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfuI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII
XmnI