##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

Acc65I      AclI        AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        Asp718I     
AspEI       AviII       BamHI       BbeI        BbrPI       
BciVI       BglI        BsaAI       BsaBI       BsiWI       
BspDI       BssHII      BstBI       BstXI       Bsu36I      
CfoI        ClaI        CpoI        Eam1105I    Eco47III    
Eco81I      EcoRV       FspI        HaeII       HhaI        
HinP1I      KasI        KpnI        KspI        MamI        
MluI        NarI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       Sse8387I    Van91I      
XhoI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII         947  GACGTC               GACGT^C
AccII        1781  CGCG                 CG^CG
AccIII       2114  TCCGGA               T^CCGGA
AcyI          947  GACGTC               G[GA]^CG[CT]C
AlwNI        1226  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI         3099  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          334  CCCGGG               C^[CT]CG[GA]G
AvrII        1758  CCTAGG               C^CTAGG
BalI         4621  TGGCCA               TGG^CCA
BcgI         4717  GCAGGAGAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          390  TGATCA               T^GATCA
BlnI         1758  CCTAGG               C^CTAGG
BlpI         3010  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     3010  GCTAAGC              GC^T[ACGT]AGC
BsaHI         947  GACGTC               G[GA]^CG[CT]C
BsaWI        2114  TCCGGA               [AT]^CCGG[AT]
BseAI        2114  TCCGGA               T^CCGGA
BsiEI         344  CGGCCG               CG[GA][CT]^CG
BsoBI         334  CCCGGG               C^[CT]CG[GA]G
BspEI        2114  TCCGGA               T^CCGGA
BspHI        1539  TCATGA               T^CATGA
BsrBI        1559  CCGCTC               CCGCTC(-3/-3)
BsrDI        4927  CATTGC               GCAATG(2/0)
BssSI        5247  CTCGTG               CACGAG(-5/-1)
BstEII        865  GGTGACC              G^GT[ACGT]ACC
BstPI         865  GGTGACC              G^GT[ACGT]ACC
BstUI        1781  CGCG                 CG^CG
CelII        3010  GCTAAGC              GC^T[ACGT]AGC
DraIII       1046  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          501  GACTAAGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          344  CGGCCG               C^GGCCG
EclXI         344  CGGCCG               C^GGCCG
Eco52I        344  CGGCCG               C^GGCCG
EcoO65I       865  GGTGACC              G^GT[ACGT]ACC
FbaI          390  TGATCA               T^GATCA
FseI         1764  GGCCGGCC             GGCCGG^CC
Hin1I         947  GACGTC               G[GA]^CG[CT]C
HpaI         3881  GTTAAC               GTT^AAC
MluNI        4621  TGGCCA               TGG^CCA
MroI         2114  TCCGGA               T^CCGGA
MscI         4621  TGGCCA               TGG^CCA
MvnI         1781  CGCG                 CG^CG
NdeI          728  CATATG               CA^TATG
NheI         3616  GCTAGC               G^CTAGC
PflFI        3099  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        5076  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI        2328  GACTAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1539  TCATGA               T^CATGA
SacI         4283  GAGCTC               GAGCT^C
ScaI         3173  AGTACT               AGT^ACT
SexAI        5133  ACCAGGT              A^CC[AT]GGT
SmaI          334  CCCGGG               CCC^GGG
SphI         1164  GCATGC               GCATG^C
SwaI         4207  ATTTAAAT             ATTT^AAAT
Tth111I      3099  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         5451  TCTAGA               T^CTAGA
XmaCI         334  CCCGGG               C^CCGGG
XmaI          334  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI         1524  GTATAC               GT^[AC][GT]AC
             5358  GTATAC              
AflII        1315  CTTAAG               C^TTAAG
             2198  CTTAAG              
AflIII       2525  ACATGT               A^C[GA][CT]GT
             5364  ACATGT              
AseI         2694  ATTAAT               AT^TAAT
             2941  ATTAAT              
AsnI         2694  ATTAAT               AT^TAAT
             2941  ATTAAT              
Asp700I       130  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3472  GAACCTTTTC          
BbvCI        1950  CCTCAGC              CCTCAGC(-5/-2)
             4711  GCTGAGG             
BfrI         1315  CTTAAG               C^TTAAG
             2198  CTTAAG              
BglII        3813  AGATCT               A^GATCT
             5260  AGATCT              
BsgI          689  CTGCAC               GTGCAG(16/14)
             4774  CTGCAC              
BsmBI        4641  CGTCTC               CGTCTC(1/5)
             4806  CGTCTC              
BspLU11I     2525  ACATGT               A^CATGT
             5364  ACATGT              
BsrFI         342  GCCGGC               [GA]^CCGG[CT]
             1765  GCCGGC              
Bst1107I     1524  GTATAC               GTA^TAC
             5358  GTATAC              
BstZ17I      1524  GTATAC               GTA^TAC
             5358  GTATAC              
Cfr10I        342  GCCGGC               [GA]^CCGG[CT]
             1765  GCCGGC              
EcoRI        1439  GAATTC               G^AATTC
             3122  GAATTC              
HgaI         1779  GACGC                GACGC(5/10)
             3054  GCGTC               
HincII       3881  GTTAAC               GT[CT]^[GA]AC
             4902  GTTGAC              
HindII       3881  GTTAAC               GT[CT]^[GA]AC
             4902  GTTGAC              
MfeI         1158  CAATTG               C^AATTG
             4099  CAATTG              
MunI         1158  CAATTG               C^AATTG
             4099  CAATTG              
NaeI          342  GCCGGC               GCC^GGC
             1765  GCCGGC              
NcoI          832  CCATGG               C^CATGG
             4886  CCATGG              
NgoMIV        342  GCCGGC               G^CCGGC
             1765  GCCGGC              
PshBI        2694  ATTAAT               AT^TAAT
             2941  ATTAAT              
SpeI         4338  ACTAGT               A^CTAGT
             4518  ACTAGT              
StuI         2720  AGGCCT               AGG^CCT
             5024  AGGCCT              
XcmI         3169  CCAAAGTACTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4304  CCAAATATCCAATGG     
XmnI          130  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3472  GAACCTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApaI        ApoI        AspHI       AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BspMI       
BsrGI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
EcoT22I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PstI        PvuII       RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII