################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## Acc65I AclI AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AviII BamHI BbeI BbrPI BciVI BglI BsaAI BsaBI BsiWI BspDI BssHII BstBI BstXI Bsu36I CfoI ClaI CpoI Eam1105I Eco47III Eco81I EcoRV FspI HaeII HhaI HinP1I KasI KpnI KspI MamI MluI NarI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI Sse8387I Van91I XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 947 GACGTC GACGT^C AccII 1781 CGCG CG^CG AccIII 2114 TCCGGA T^CCGGA AcyI 947 GACGTC G[GA]^CG[CT]C AlwNI 1226 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 3099 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 334 CCCGGG C^[CT]CG[GA]G AvrII 1758 CCTAGG C^CTAGG BalI 4621 TGGCCA TGG^CCA BcgI 4717 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 390 TGATCA T^GATCA BlnI 1758 CCTAGG C^CTAGG BlpI 3010 GCTAAGC GC^T[ACGT]AGC Bpu1102I 3010 GCTAAGC GC^T[ACGT]AGC BsaHI 947 GACGTC G[GA]^CG[CT]C BsaWI 2114 TCCGGA [AT]^CCGG[AT] BseAI 2114 TCCGGA T^CCGGA BsiEI 344 CGGCCG CG[GA][CT]^CG BsoBI 334 CCCGGG C^[CT]CG[GA]G BspEI 2114 TCCGGA T^CCGGA BspHI 1539 TCATGA T^CATGA BsrBI 1559 CCGCTC CCGCTC(-3/-3) BsrDI 4927 CATTGC GCAATG(2/0) BssSI 5247 CTCGTG CACGAG(-5/-1) BstEII 865 GGTGACC G^GT[ACGT]ACC BstPI 865 GGTGACC G^GT[ACGT]ACC BstUI 1781 CGCG CG^CG CelII 3010 GCTAAGC GC^T[ACGT]AGC DraIII 1046 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 501 GACTAAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 344 CGGCCG C^GGCCG EclXI 344 CGGCCG C^GGCCG Eco52I 344 CGGCCG C^GGCCG EcoO65I 865 GGTGACC G^GT[ACGT]ACC FbaI 390 TGATCA T^GATCA FseI 1764 GGCCGGCC GGCCGG^CC Hin1I 947 GACGTC G[GA]^CG[CT]C HpaI 3881 GTTAAC GTT^AAC MluNI 4621 TGGCCA TGG^CCA MroI 2114 TCCGGA T^CCGGA MscI 4621 TGGCCA TGG^CCA MvnI 1781 CGCG CG^CG NdeI 728 CATATG CA^TATG NheI 3616 GCTAGC G^CTAGC PflFI 3099 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 5076 AGGACCT [GA]G^G[AT]CC[CT] PshAI 2328 GACTAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1539 TCATGA T^CATGA SacI 4283 GAGCTC GAGCT^C ScaI 3173 AGTACT AGT^ACT SexAI 5133 ACCAGGT A^CC[AT]GGT SmaI 334 CCCGGG CCC^GGG SphI 1164 GCATGC GCATG^C SwaI 4207 ATTTAAAT ATTT^AAAT Tth111I 3099 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5451 TCTAGA T^CTAGA XmaCI 334 CCCGGG C^CCGGG XmaI 334 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1524 GTATAC GT^[AC][GT]AC 5358 GTATAC AflII 1315 CTTAAG C^TTAAG 2198 CTTAAG AflIII 2525 ACATGT A^C[GA][CT]GT 5364 ACATGT AseI 2694 ATTAAT AT^TAAT 2941 ATTAAT AsnI 2694 ATTAAT AT^TAAT 2941 ATTAAT Asp700I 130 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3472 GAACCTTTTC BbvCI 1950 CCTCAGC CCTCAGC(-5/-2) 4711 GCTGAGG BfrI 1315 CTTAAG C^TTAAG 2198 CTTAAG BglII 3813 AGATCT A^GATCT 5260 AGATCT BsgI 689 CTGCAC GTGCAG(16/14) 4774 CTGCAC BsmBI 4641 CGTCTC CGTCTC(1/5) 4806 CGTCTC BspLU11I 2525 ACATGT A^CATGT 5364 ACATGT BsrFI 342 GCCGGC [GA]^CCGG[CT] 1765 GCCGGC Bst1107I 1524 GTATAC GTA^TAC 5358 GTATAC BstZ17I 1524 GTATAC GTA^TAC 5358 GTATAC Cfr10I 342 GCCGGC [GA]^CCGG[CT] 1765 GCCGGC EcoRI 1439 GAATTC G^AATTC 3122 GAATTC HgaI 1779 GACGC GACGC(5/10) 3054 GCGTC HincII 3881 GTTAAC GT[CT]^[GA]AC 4902 GTTGAC HindII 3881 GTTAAC GT[CT]^[GA]AC 4902 GTTGAC MfeI 1158 CAATTG C^AATTG 4099 CAATTG MunI 1158 CAATTG C^AATTG 4099 CAATTG NaeI 342 GCCGGC GCC^GGC 1765 GCCGGC NcoI 832 CCATGG C^CATGG 4886 CCATGG NgoMIV 342 GCCGGC G^CCGGC 1765 GCCGGC PshBI 2694 ATTAAT AT^TAAT 2941 ATTAAT SpeI 4338 ACTAGT A^CTAGT 4518 ACTAGT StuI 2720 AGGCCT AGG^CCT 5024 AGGCCT XcmI 3169 CCAAAGTACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4304 CCAAATATCCAATGG XmnI 130 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3472 GAACCTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApaI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII