##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp718I
AspEI AviII BamHI BbeI BbrPI
BciVI BglI BsaAI BsaBI BsiWI
BspDI BssHII BstBI BstXI Bsu36I
CfoI ClaI CpoI Eam1105I Eco47III
Eco81I EcoRV FspI HaeII HhaI
HinP1I KasI KpnI KspI MamI
MluI NarI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 947 GACGTC GACGT^C
AccII 1781 CGCG CG^CG
AccIII 2114 TCCGGA T^CCGGA
AcyI 947 GACGTC G[GA]^CG[CT]C
AlwNI 1226 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 3099 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 334 CCCGGG C^[CT]CG[GA]G
AvrII 1758 CCTAGG C^CTAGG
BalI 4621 TGGCCA TGG^CCA
BcgI 4717 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 390 TGATCA T^GATCA
BlnI 1758 CCTAGG C^CTAGG
BlpI 3010 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3010 GCTAAGC GC^T[ACGT]AGC
BsaHI 947 GACGTC G[GA]^CG[CT]C
BsaWI 2114 TCCGGA [AT]^CCGG[AT]
BseAI 2114 TCCGGA T^CCGGA
BsiEI 344 CGGCCG CG[GA][CT]^CG
BsoBI 334 CCCGGG C^[CT]CG[GA]G
BspEI 2114 TCCGGA T^CCGGA
BspHI 1539 TCATGA T^CATGA
BsrBI 1559 CCGCTC CCGCTC(-3/-3)
BsrDI 4927 CATTGC GCAATG(2/0)
BssSI 5247 CTCGTG CACGAG(-5/-1)
BstEII 865 GGTGACC G^GT[ACGT]ACC
BstPI 865 GGTGACC G^GT[ACGT]ACC
BstUI 1781 CGCG CG^CG
CelII 3010 GCTAAGC GC^T[ACGT]AGC
DraIII 1046 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 501 GACTAAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 344 CGGCCG C^GGCCG
EclXI 344 CGGCCG C^GGCCG
Eco52I 344 CGGCCG C^GGCCG
EcoO65I 865 GGTGACC G^GT[ACGT]ACC
FbaI 390 TGATCA T^GATCA
FseI 1764 GGCCGGCC GGCCGG^CC
Hin1I 947 GACGTC G[GA]^CG[CT]C
HpaI 3881 GTTAAC GTT^AAC
MluNI 4621 TGGCCA TGG^CCA
MroI 2114 TCCGGA T^CCGGA
MscI 4621 TGGCCA TGG^CCA
MvnI 1781 CGCG CG^CG
NdeI 728 CATATG CA^TATG
NheI 3616 GCTAGC G^CTAGC
PflFI 3099 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 5076 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2328 GACTAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1539 TCATGA T^CATGA
SacI 4283 GAGCTC GAGCT^C
ScaI 3173 AGTACT AGT^ACT
SexAI 5133 ACCAGGT A^CC[AT]GGT
SmaI 334 CCCGGG CCC^GGG
SphI 1164 GCATGC GCATG^C
SwaI 4207 ATTTAAAT ATTT^AAAT
Tth111I 3099 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5451 TCTAGA T^CTAGA
XmaCI 334 CCCGGG C^CCGGG
XmaI 334 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1524 GTATAC GT^[AC][GT]AC
5358 GTATAC
AflII 1315 CTTAAG C^TTAAG
2198 CTTAAG
AflIII 2525 ACATGT A^C[GA][CT]GT
5364 ACATGT
AseI 2694 ATTAAT AT^TAAT
2941 ATTAAT
AsnI 2694 ATTAAT AT^TAAT
2941 ATTAAT
Asp700I 130 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3472 GAACCTTTTC
BbvCI 1950 CCTCAGC CCTCAGC(-5/-2)
4711 GCTGAGG
BfrI 1315 CTTAAG C^TTAAG
2198 CTTAAG
BglII 3813 AGATCT A^GATCT
5260 AGATCT
BsgI 689 CTGCAC GTGCAG(16/14)
4774 CTGCAC
BsmBI 4641 CGTCTC CGTCTC(1/5)
4806 CGTCTC
BspLU11I 2525 ACATGT A^CATGT
5364 ACATGT
BsrFI 342 GCCGGC [GA]^CCGG[CT]
1765 GCCGGC
Bst1107I 1524 GTATAC GTA^TAC
5358 GTATAC
BstZ17I 1524 GTATAC GTA^TAC
5358 GTATAC
Cfr10I 342 GCCGGC [GA]^CCGG[CT]
1765 GCCGGC
EcoRI 1439 GAATTC G^AATTC
3122 GAATTC
HgaI 1779 GACGC GACGC(5/10)
3054 GCGTC
HincII 3881 GTTAAC GT[CT]^[GA]AC
4902 GTTGAC
HindII 3881 GTTAAC GT[CT]^[GA]AC
4902 GTTGAC
MfeI 1158 CAATTG C^AATTG
4099 CAATTG
MunI 1158 CAATTG C^AATTG
4099 CAATTG
NaeI 342 GCCGGC GCC^GGC
1765 GCCGGC
NcoI 832 CCATGG C^CATGG
4886 CCATGG
NgoMIV 342 GCCGGC G^CCGGC
1765 GCCGGC
PshBI 2694 ATTAAT AT^TAAT
2941 ATTAAT
SpeI 4338 ACTAGT A^CTAGT
4518 ACTAGT
StuI 2720 AGGCCT AGG^CCT
5024 AGGCCT
XcmI 3169 CCAAAGTACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4304 CCAAATATCCAATGG
XmnI 130 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3472 GAACCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII