##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AspEI
BglI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BssHII BstBI BstEII
BstPI ClaI CpoI DrdI Eam1105I
Eco47III EcoO65I EcoRV EcoT22I FseI
HpaI KspI MfeI MluI MroI
MunI NcoI NdeI NotI NruI
NsiI NspV PacI PinAI PmeI
Psp1406I PvuI RsrII SacII ScaI
SfiI SfuI SgrAI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 5378 GGTACC G^GTACC
AflII 2480 CTTAAG C^TTAAG
AseI 5910 ATTAAT AT^TAAT
AsnI 5910 ATTAAT AT^TAAT
Asp718I 5378 GGTACC G^GTACC
AspI 4261 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 778 TGCGCA TGC^GCA
BamHI 5282 GGATCC G^GATCC
BbeI 180 GGCGCC GGCGC^C
BbrPI 4056 CACGTG CAC^GTG
BbsI 4851 GTCTTC GAAGAC(2/6)
BbvCI 351 GCTGAGG CCTCAGC(-5/-2)
BcgI 4790 CGAGGTAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2480 CTTAAG C^TTAAG
BlpI 860 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 860 GCTGAGC GC^T[ACGT]AGC
BpuAI 4851 GTCTTC GAAGAC(2/6)
BsaBI 800 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 141 CGGCCG CG[GA][CT]^CG
Bsp1407I 2198 TGTACA T^GTACA
BspLU11I 5818 ACATGT A^CATGT
BsrDI 1239 CATTGC GCAATG(2/0)
BsrFI 143 GCCGGC [GA]^CCGG[CT]
BsrGI 2198 TGTACA T^GTACA
BssSI 1948 CTCGTG CACGAG(-5/-1)
CelII 860 GCTGAGC GC^T[ACGT]AGC
Cfr10I 143 GCCGGC [GA]^CCGG[CT]
EagI 141 CGGCCG C^GGCCG
EclXI 141 CGGCCG C^GGCCG
Eco52I 141 CGGCCG C^GGCCG
EcoRI 4957 GAATTC G^AATTC
FspI 778 TGCGCA TGC^GCA
HincII 2000 GTCGAC GT[CT]^[GA]AC
HindII 2000 GTCGAC GT[CT]^[GA]AC
HindIII 6154 AAGCTT A^AGCTT
KasI 180 GGCGCC G^GCGCC
KpnI 5378 GGTACC GGTAC^C
MamI 800 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 143 GCCGGC GCC^GGC
NarI 180 GGCGCC GG^CGCC
NgoMIV 143 GCCGGC G^CCGGC
NheI 1513 GCTAGC G^CTAGC
PaeR7I 3681 CTCGAG C^TCGAG
PflFI 4261 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4056 CACGTG CAC^GTG
PmlI 4056 CACGTG CAC^GTG
PshAI 4450 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5910 ATTAAT AT^TAAT
SalI 2000 GTCGAC G^TCGAC
SbfI 3041 CCTGCAGG CCTGCA^GG
SexAI 2053 ACCAGGT A^CC[AT]GGT
SfoI 180 GGCGCC GGC^GCC
SnaBI 1705 TACGTA TAC^GTA
SphI 3125 GCATGC GCATG^C
Sse8387I 3041 CCTGCAGG CCTGCA^GG
SspBI 2198 TGTACA T^GTACA
Tth111I 4261 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3681 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 180 GGCGCC G[GA]^CG[CT]C
963 GGCGTC
AflIII 1733 ACGTGT A^C[GA][CT]GT
5818 ACATGT
Asp700I 281 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4289 GAAGTCGTTC
AvrII 3342 CCTAGG C^CTAGG
3430 CCTAGG
BalI 253 TGGCCA TGG^CCA
2792 TGGCCA
BciVI 1085 GTATCC GTATCC(6/5)
3284 GTATCC
BclI 1655 TGATCA T^GATCA
3489 TGATCA
BlnI 3342 CCTAGG C^CTAGG
3430 CCTAGG
BsaHI 180 GGCGCC G[GA]^CG[CT]C
963 GGCGTC
BsaI 1975 GAGACC GGTCTC(1/5)
2947 GGTCTC
BsmI 1540 GCATTC GAATGC(1/-1)
2901 GCATTC
BspHI 1934 TCATGA T^CATGA
4446 TCATGA
BsrBI 123 GAGCGG CCGCTC(-3/-3)
518 GAGCGG
Bst1107I 2489 GTATAC GTA^TAC
6148 GTATAC
BstAPI 577 GCATCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3670 GCAGTCACTGC
BstZ17I 2489 GTATAC GTA^TAC
6148 GTATAC
DraIII 4079 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5625 CACTGTGTG
FbaI 1655 TGATCA T^GATCA
3489 TGATCA
Hin1I 180 GGCGCC G[GA]^CG[CT]C
963 GGCGTC
MluNI 253 TGGCCA TGG^CCA
2792 TGGCCA
MscI 253 TGGCCA TGG^CCA
2792 TGGCCA
PvuII 743 CAGCTG CAG^CTG
1644 CAGCTG
RcaI 1934 TCATGA T^CATGA
4446 TCATGA
SapI 320 GAAGAGC GCTCTTC(1/4)
3358 GCTCTTC
SmaI 1624 CCCGGG CCC^GGG
5374 CCCGGG
SspI 6033 AATATT AAT^ATT
6244 AATATT
StuI 3427 AGGCCT AGG^CCT
4208 AGGCCT
XbaI 3443 TCTAGA T^CTAGA
4240 TCTAGA
XmaCI 1624 CCCGGG C^CCGGG
5374 CCCGGG
XmaI 1624 CCCGGG C^CCGGG
5374 CCCGGG
XmnI 281 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4289 GAAGTCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BglII
BmyI BpmI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII