##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccIII AclI AflII AscI
Asp718I AviII AvrII BbrPI BclI
BfrI BglII BlnI BseAI Bsp1407I
BspEI BsrBI BsrGI BssHII BssSI
BstBI CpoI DrdI EagI EclXI
Eco52I EcoT22I FbaI FseI FspI
KpnI KspI MfeI MroI MunI
NaeI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmlI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfuI SgrAI SmaI SpeI Sse8387I
SspBI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AgeI 3427 ACCGGT A^CCGGT
AhdI 3416 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4579 AGCGCT AGC^GCT
AseI 1054 ATTAAT AT^TAAT
AsnI 1054 ATTAAT AT^TAAT
AspEI 3416 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4202 GGATCC G^GATCC
BbeI 4396 GGCGCC GGCGC^C
BcgI 2136 GCAGACAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 540 GTATCC GTATCC(6/5)
BglI 3903 GCCTTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4061 TACGTA [CT]AC^GT[GA]
BsaBI 4197 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3427 ACCGGT [AT]^CCGG[AT]
BsiEI 4898 CGACCG CG[GA][CT]^CG
BsiWI 3942 CGTACG C^GTACG
BspHI 1695 TCATGA T^CATGA
BstAPI 4568 GCATCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 3343 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3638 CCACGG C^C[GA][CT]GG
Eam1105I 3416 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4579 AGCGCT AGC^GCT
EcoRV 1215 GATATC GAT^ATC
HindIII 3625 AAGCTT A^AGCTT
KasI 4396 GGCGCC G^GCGCC
MamI 4197 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4730 ACGCGT A^CGCGT
NarI 4396 GGCGCC GG^CGCC
NheI 4973 GCTAGC G^CTAGC
PinAI 3427 ACCGGT A^CCGGT
PmeI 5128 GTTTAAAC GTTT^AAAC
PshAI 4899 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1054 ATTAAT AT^TAAT
PstI 4464 CTGCAG CTGCA^G
PvuII 4077 CAGCTG CAG^CTG
RcaI 1695 TCATGA T^CATGA
SacI 4515 GAGCTC GAGCT^C
ScaI 546 AGTACT AGT^ACT
SexAI 993 ACCAGGT A^CC[AT]GGT
SfoI 4396 GGCGCC GGC^GCC
SnaBI 4061 TACGTA TAC^GTA
SphI 738 GCATGC GCATG^C
StuI 3591 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3616 GACGTC GACGT^C
4807 GACGTC
Alw44I 1199 GTGCAC G^TGCAC
1429 GTGCAC
AocI 1516 CCTCAGG CC^T[ACGT]AGG
2390 CCTCAGG
ApaLI 1199 GTGCAC G^TGCAC
1429 GTGCAC
AspI 157 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1306 GACGGAGTC
AvaI 2902 CCCGAG C^[CT]CG[GA]G
4623 CCCGAG
BalI 1490 TGGCCA TGG^CCA
1947 TGGCCA
BanI 2188 GGTGCC G^G[CT][GA]CC
4396 GGCGCC
BlpI 4505 GCTCAGC GC^T[ACGT]AGC
4517 GCTCAGC
Bpu1102I 4505 GCTCAGC GC^T[ACGT]AGC
4517 GCTCAGC
BsmI 1335 GAATGC GAATGC(1/-1)
1656 GCATTC
BsoBI 2902 CCCGAG C^[CT]CG[GA]G
4623 CCCGAG
BspDI 4386 ATCGAT AT^CGAT
5147 ATCGAT
Bst1107I 228 GTATAC GTA^TAC
2513 GTATAC
BstEII 2238 GGTCACC G^GT[ACGT]ACC
2395 GGTAACC
BstPI 2238 GGTCACC G^GT[ACGT]ACC
2395 GGTAACC
BstXI 1409 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1544 CCAAAGTGCTGG
BstZ17I 228 GTATAC GTA^TAC
2513 GTATAC
Bsu36I 1516 CCTCAGG CC^T[ACGT]AGG
2390 CCTCAGG
CelII 4505 GCTCAGC GC^T[ACGT]AGC
4517 GCTCAGC
ClaI 4386 ATCGAT AT^CGAT
5147 ATCGAT
Eco81I 1516 CCTCAGG CC^T[ACGT]AGG
2390 CCTCAGG
EcoNI 2063 CCTGTCGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3371 CCTGAGCAAGG
EcoO65I 2238 GGTCACC G^GT[ACGT]ACC
2395 GGTAACC
EcoRI 85 GAATTC G^AATTC
1959 GAATTC
HaeII 4396 GGCGCC [GA]GCGC^[CT]
4579 AGCGCT
MluNI 1490 TGGCCA TGG^CCA
1947 TGGCCA
MscI 1490 TGGCCA TGG^CCA
1947 TGGCCA
PflFI 157 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1306 GACGGAGTC
PflMI 2113 CCACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3342 CCACCCTGTGG
Tth111I 157 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1306 GACGGAGTC
Van91I 2113 CCACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3342 CCACCCTGTGG
XcmI 2113 CCACAGGTTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4189 CCAAGTATGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI Asp700I AspHI AvaII
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspLU11I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI