##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AccIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AseI AsnI
AspI BseAI BsiWI BspDI BspEI
BstAPI BstBI Bsu36I ClaI CpoI
DraIII Eco47III Eco81I EcoRV HindIII
HpaI MfeI MluI MroI MunI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
PvuI RsrII SacI SfiI SfuI
SnaBI SpeI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 5020 GGTACC G^GTACC
AclI 3369 AACGTT AA^CGTT
AflII 4409 CTTAAG C^TTAAG
AflIII 2374 ACATGT A^C[GA][CT]GT
AhdI 855 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 2887 GGCGCGCC GG^CGCGCC
Asp718I 5020 GGTACC G^GTACC
AspEI 855 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4103 CCTAGG C^CTAGG
BbeI 311 GGCGCC GGCGC^C
BbrPI 1473 CACGTG CAC^GTG
BbsI 4064 GAAGAC GAAGAC(2/6)
BfrI 4409 CTTAAG C^TTAAG
BlnI 4103 CCTAGG C^CTAGG
BpuAI 4064 GAAGAC GAAGAC(2/6)
BsaAI 1473 CACGTG [CT]AC^GT[GA]
BsaBI 2186 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 305 CGTCTC CGTCTC(1/5)
Bsp1407I 3255 TGTACA T^GTACA
BspLU11I 2374 ACATGT A^CATGT
BsrGI 3255 TGTACA T^GTACA
Bst1107I 5628 GTATAC GTA^TAC
BstZ17I 5628 GTATAC GTA^TAC
DrdI 5136 GACACTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 855 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 5414 ATGCAT ATGCA^T
FseI 82 GGCCGGCC GGCCGG^CC
KasI 311 GGCGCC G^GCGCC
KpnI 5020 GGTACC GGTAC^C
MamI 2186 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 311 GGCGCC GG^CGCC
NdeI 1881 CATATG CA^TATG
NsiI 5414 ATGCAT ATGCA^T
PmaCI 1473 CACGTG CAC^GTG
PmlI 1473 CACGTG CAC^GTG
PshAI 583 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3369 AACGTT AA^CGTT
SalI 3263 GTCGAC G^TCGAC
SbfI 3842 CCTGCAGG CCTGCA^GG
SexAI 4604 ACCAGGT A^CC[AT]GGT
SfoI 311 GGCGCC GGC^GCC
SgrAI 2880 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 886 CCCGGG CCC^GGG
Sse8387I 3842 CCTGCAGG CCTGCA^GG
SspBI 3255 TGTACA T^GTACA
XmaCI 886 CCCGGG C^CCGGG
XmaI 886 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3263 GTCGAC GT^[AC][GT]AC
5628 GTATAC
Asp700I 758 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3594 GAAGTTTTTC
AviII 1348 TGCGCA TGC^GCA
2842 TGCGCA
BamHI 751 GGATCC G^GATCC
3284 GGATCC
BbvCI 1487 CCTCAGC CCTCAGC(-5/-2)
4503 CCTCAGC
BcgI 2551 GCATGCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3093 GCACGGCCGTCG
BclI 2185 TGATCA T^GATCA
5366 TGATCA
BglII 1312 AGATCT A^GATCT
1778 AGATCT
BlpI 109 GCTCAGC GC^T[ACGT]AGC
2954 GCTCAGC
Bpu1102I 109 GCTCAGC GC^T[ACGT]AGC
2954 GCTCAGC
BspMI 1345 ACCTGC ACCTGC(4/8)
5252 ACCTGC
BsrDI 3901 CATTGC GCAATG(2/0)
1615 GCAATG
BssHII 2888 GCGCGC G^CGCGC
4370 GCGCGC
BssSI 339 CTCGTG CACGAG(-5/-1)
5171 CTCGTG
BstEII 2831 GGTGACC G^GT[ACGT]ACC
4600 GGTTACC
BstPI 2831 GGTGACC G^GT[ACGT]ACC
4600 GGTTACC
CelII 109 GCTCAGC GC^T[ACGT]AGC
2954 GCTCAGC
EagI 81 CGGCCG C^GGCCG
3096 CGGCCG
EclXI 81 CGGCCG C^GGCCG
3096 CGGCCG
Eco52I 81 CGGCCG C^GGCCG
3096 CGGCCG
EcoO65I 2831 GGTGACC G^GT[ACGT]ACC
4600 GGTTACC
EcoRI 1386 GAATTC G^AATTC
2438 GAATTC
FbaI 2185 TGATCA T^GATCA
5366 TGATCA
FspI 1348 TGCGCA TGC^GCA
2842 TGCGCA
NaeI 83 GCCGGC GCC^GGC
2881 GCCGGC
NcoI 397 CCATGG C^CATGG
4152 CCATGG
NgoMIV 83 GCCGGC G^CCGGC
2881 GCCGGC
SapI 2405 GAAGAGC GCTCTTC(1/4)
1940 GCTCTTC
ScaI 2065 AGTACT AGT^ACT
2479 AGTACT
SphI 871 GCATGC GCATG^C
2551 GCATGC
StuI 1793 AGGCCT AGG^CCT
2690 AGGCCT
TaqI 487 TCGA T^CGA
3264 TCGA
TthHB8I 487 TCGA T^CGA
3264 TCGA
XmnI 758 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3594 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BciVI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII