##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BciVI BclI BlnI BsaBI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrGI BssHII Bst1107I BstEII BstPI
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoO65I EcoRV FbaI HgaI HincII
HindII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NdeI
NotI NruI PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccII 5679 CGCG CG^CG
AcyI 3623 GGCGCC G[GA]^CG[CT]C
AflII 1020 CTTAAG C^TTAAG
AviII 2533 TGCGCA TGC^GCA
BbeI 3623 GGCGCC GGCGC^C
BbrPI 5559 CACGTG CAC^GTG
BfrI 1020 CTTAAG C^TTAAG
BsaHI 3623 GGCGCC G[GA]^CG[CT]C
BsaWI 1938 ACCGGA [AT]^CCGG[AT]
BspHI 3190 TCATGA T^CATGA
BstUI 5679 CGCG CG^CG
DraI 5503 TTTAAA TTT^AAA
DrdI 1834 GACTCGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 5024 GAATTC G^AATTC
EcoT22I 5050 ATGCAT ATGCA^T
FseI 12 GGCCGGCC GGCCGG^CC
FspI 2533 TGCGCA TGC^GCA
Hin1I 3623 GGCGCC G[GA]^CG[CT]C
KasI 3623 GGCGCC G^GCGCC
MvnI 5679 CGCG CG^CG
NarI 3623 GGCGCC GG^CGCC
NheI 1857 GCTAGC G^CTAGC
NsiI 5050 ATGCAT ATGCA^T
PmaCI 5559 CACGTG CAC^GTG
PmlI 5559 CACGTG CAC^GTG
PshAI 3956 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3190 TCATGA T^CATGA
SfoI 3623 GGCGCC GGC^GCC
SmaI 2683 CCCGGG CCC^GGG
SspI 564 AATATT AAT^ATT
StuI 3710 AGGCCT AGG^CCT
XmaCI 2683 CCCGGG C^CCGGG
XmaI 2683 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 5024 GAATTC [GA]^AATT[CT]
5113 AAATTT
ApoI 5024 GAATTC [GA]^AATT[CT]
5113 AAATTT
Asp700I 1412 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5340 GAAGTGCTTC
AspI 3837 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3873 GACAAGGTC
BbsI 366 GAAGAC GAAGAC(2/6)
3953 GAAGAC
BbvCI 4890 CCTCAGC CCTCAGC(-5/-2)
393 GCTGAGG
BcgI 767 GCATCAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2605 GCAAGATGCTCG
BglII 2369 AGATCT A^GATCT
3038 AGATCT
BpuAI 366 GAAGAC GAAGAC(2/6)
3953 GAAGAC
BsaAI 5559 CACGTG [CT]AC^GT[GA]
5669 TACGTG
BsiEI 1733 CGGCCG CG[GA][CT]^CG
5526 CGGCCG
BsmI 1886 GAATGC GAATGC(1/-1)
3296 GAATGC
BspLU11I 1454 ACATGT A^CATGT
4966 ACATGT
BssSI 1446 CACGAG CACGAG(-5/-1)
3301 CTCGTG
BstBI 1510 TTCGAA TT^CGAA
2704 TTCGAA
BstXI 3745 CCACCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3758 CCAGACTGCTGG
EagI 1733 CGGCCG C^GGCCG
5526 CGGCCG
EclXI 1733 CGGCCG C^GGCCG
5526 CGGCCG
Eco52I 1733 CGGCCG C^GGCCG
5526 CGGCCG
HindIII 2998 AAGCTT A^AGCTT
3208 AAGCTT
NaeI 13 GCCGGC GCC^GGC
1731 GCCGGC
NcoI 388 CCATGG C^CATGG
1195 CCATGG
NgoMIV 13 GCCGGC G^CCGGC
1731 GCCGGC
NspV 1510 TTCGAA TT^CGAA
2704 TTCGAA
PflFI 3837 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3873 GACAAGGTC
PflMI 4439 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4763 CCACCCCCTGG
SapI 831 GAAGAGC GCTCTTC(1/4)
1650 GAAGAGC
SfiI 1394 GGCCTGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3711 GGCCTCCTCGGCC
SfuI 1510 TTCGAA TT^CGAA
2704 TTCGAA
Tsp45I 2266 GTGAC ^GT[GC]AC
2311 GTCAC
Tth111I 3837 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3873 GACAAGGTC
Van91I 4439 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4763 CCACCCCCTGG
XbaI 1283 TCTAGA T^CTAGA
5222 TCTAGA
XmnI 1412 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5340 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XcmI XhoII