##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII AclI AflII AgeI Aor51HI
AscI AseI AsnI Asp700I AviII
BfrI BglII BsiWI BspDI BssHII
BstBI ClaI Eco47III EcoRV EcoT22I
FspI HindIII HpaI MfeI MluI
MunI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI SalI SfuI SgrAI SnaBI
SpeI SspI StuI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 2337 TCCGGA T^CCGGA
AhdI 2009 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3783 GTGCAC G^TGCAC
ApaLI 3783 GTGCAC G^TGCAC
AspEI 2009 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2522 GGCGCC GGCGC^C
BbrPI 1984 CACGTG CAC^GTG
BciVI 2754 GTATCC GTATCC(6/5)
BsaAI 1984 CACGTG [CT]AC^GT[GA]
BseAI 2337 TCCGGA T^CCGGA
BsiEI 75 CGGCCG CG[GA][CT]^CG
BsmBI 2451 GAGACG CGTCTC(1/5)
Bsp1407I 804 TGTACA T^GTACA
BspEI 2337 TCCGGA T^CCGGA
BspLU11I 3664 ACATGT A^CATGT
BsrGI 804 TGTACA T^GTACA
BssSI 2639 CTCGTG CACGAG(-5/-1)
BstEII 997 GGTCACC G^GT[ACGT]ACC
BstPI 997 GGTCACC G^GT[ACGT]ACC
CpoI 2811 CGGACCG CG^G[AT]CCG
EagI 75 CGGCCG C^GGCCG
Eam1105I 2009 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 75 CGGCCG C^GGCCG
Eco52I 75 CGGCCG C^GGCCG
EcoNI 2475 CCTTCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 997 GGTCACC G^GT[ACGT]ACC
EcoRI 629 GAATTC G^AATTC
FseI 76 GGCCGGCC GGCCGG^CC
KasI 2522 GGCGCC G^GCGCC
MroI 2337 TCCGGA T^CCGGA
NarI 2522 GGCGCC GG^CGCC
NdeI 5084 CATATG CA^TATG
NheI 1456 GCTAGC G^CTAGC
PmaCI 1984 CACGTG CAC^GTG
PmlI 1984 CACGTG CAC^GTG
PshAI 767 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2811 CGGACCG CG^G[AT]CCG
SbfI 2174 CCTGCAGG CCTGCA^GG
ScaI 2535 AGTACT AGT^ACT
SexAI 510 ACCTGGT A^CC[AT]GGT
SfoI 2522 GGCGCC GGC^GCC
SphI 407 GCATGC GCATG^C
Sse8387I 2174 CCTGCAGG CCTGCA^GG
SspBI 804 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 458 GGTACC G^GTACC
1433 GGTACC
AflIII 1983 ACACGT A^C[GA][CT]GT
3664 ACATGT
AocI 2370 CCTCAGG CC^T[ACGT]AGG
3946 CCTGAGG
Asp718I 458 GGTACC G^GTACC
1433 GGTACC
AspI 3878 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4123 GACCCTGTC
BamHI 317 GGATCC G^GATCC
3207 GGATCC
BclI 1647 TGATCA T^GATCA
4845 TGATCA
BsaWI 1908 ACCGGA [AT]^CCGG[AT]
2337 TCCGGA
BsmI 4359 GAATGC GAATGC(1/-1)
4366 GCATTC
BspHI 519 TCATGA T^CATGA
664 TCATGA
BsrBI 4469 CCGCTC CCGCTC(-3/-3)
18 GAGCGG
BsrDI 808 CATTGC GCAATG(2/0)
2223 GCAATG
Bst1107I 4443 GTATAC GTA^TAC
4552 GTATAC
BstAPI 394 GCAGGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
536 GCACAGGATGC
BstZ17I 4443 GTATAC GTA^TAC
4552 GTATAC
Bsu36I 2370 CCTCAGG CC^T[ACGT]AGG
3946 CCTGAGG
DrdI 2422 GACGCCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3026 GACCCTGTGGTC
Eco81I 2370 CCTCAGG CC^T[ACGT]AGG
3946 CCTGAGG
FbaI 1647 TGATCA T^GATCA
4845 TGATCA
HincII 1607 GTCAAC GT[CT]^[GA]AC
2414 GTCAAC
HindII 1607 GTCAAC GT[CT]^[GA]AC
2414 GTCAAC
KpnI 458 GGTACC GGTAC^C
1433 GGTACC
NaeI 77 GCCGGC GCC^GGC
1280 GCCGGC
NgoMIV 77 GCCGGC G^CCGGC
1280 GCCGGC
PaeR7I 3856 CTCGAG C^TCGAG
4709 CTCGAG
PflFI 3878 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4123 GACCCTGTC
RcaI 519 TCATGA T^CATGA
664 TCATGA
SapI 2657 GAAGAGC GCTCTTC(1/4)
189 GCTCTTC
SfiI 28 GGCCGCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
71 GGCCCGGCCGGCC
Tth111I 3878 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4123 GACCCTGTC
XhoI 3856 CTCGAG C^TCGAG
4709 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII AvrII
BalI BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI