##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
ApoI AscI AseI AsnI AviII
BciVI BclI BfrI BsaBI BseAI
BsiEI BsmBI BsmI BspDI BspEI
BspHI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KspI MamI MluI MroI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfuI SnaBI SpeI
Sse8387I SspI SwaI TfiI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 18 enzymes) #
###################################
AgeI 3347 ACCGGT A^CCGGT
AhdI 4489 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4489 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3092 CCTAGG C^CTAGG
BamHI 2950 GGATCC G^GATCC
BbsI 1865 GAAGAC GAAGAC(2/6)
BcgI 3635 CGACACTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3092 CCTAGG C^CTAGG
BpuAI 1865 GAAGAC GAAGAC(2/6)
BsaWI 3347 ACCGGT [AT]^CCGG[AT]
BsiWI 3930 CGTACG C^GTACG
DraI 4729 TTTAAA TTT^AAA
Eam1105I 4489 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 237 GTCAAC GT[CT]^[GA]AC
HindII 237 GTCAAC GT[CT]^[GA]AC
PinAI 3347 ACCGGT A^CCGGT
ScaI 1846 AGTACT AGT^ACT
SfiI 4315 GGCCTGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1161 GGTACC G^GTACC
1342 GGTACC
AccI 2763 GTAGAC GT^[AC][GT]AC
3925 GTAGAC
Asp718I 1161 GGTACC G^GTACC
1342 GGTACC
AspI 2070 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2766 GACCCAGTC
BbeI 1153 GGCGCC GGCGC^C
3359 GGCGCC
BbrPI 732 CACGTG CAC^GTG
1596 CACGTG
BbvCI 2659 CCTCAGC CCTCAGC(-5/-2)
4671 CCTCAGC
BlpI 263 GCTCAGC GC^T[ACGT]AGC
4590 GCTAAGC
Bpu1102I 263 GCTCAGC GC^T[ACGT]AGC
4590 GCTAAGC
BsaAI 732 CACGTG [CT]AC^GT[GA]
1596 CACGTG
BspLU11I 1476 ACATGT A^CATGT
3549 ACATGT
CelII 263 GCTCAGC GC^T[ACGT]AGC
4590 GCTAAGC
HindIII 1486 AAGCTT A^AGCTT
1497 AAGCTT
KasI 1153 GGCGCC G^GCGCC
3359 GGCGCC
KpnI 1161 GGTACC GGTAC^C
1342 GGTACC
MfeI 3444 CAATTG C^AATTG
3908 CAATTG
MunI 3444 CAATTG C^AATTG
3908 CAATTG
NarI 1153 GGCGCC GG^CGCC
3359 GGCGCC
NheI 98 GCTAGC G^CTAGC
3464 GCTAGC
PflFI 2070 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2766 GACCCAGTC
PmaCI 732 CACGTG CAC^GTG
1596 CACGTG
PmlI 732 CACGTG CAC^GTG
1596 CACGTG
SacI 1025 GAGCTC GAGCT^C
4513 GAGCTC
SapI 648 GAAGAGC GCTCTTC(1/4)
2189 GCTCTTC
SfoI 1153 GGCGCC GGC^GCC
3359 GGCGCC
SgrAI 1944 CACCGGCG C[GA]^CCGG[CT]G
3346 CACCGGTG
SphI 1378 GCATGC GCATG^C
5177 GCATGC
TaqI 586 TCGA T^CGA
1852 TCGA
Tth111I 2070 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2766 GACCCAGTC
TthHB8I 586 TCGA T^CGA
1852 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BglII
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmaI SmlI
SspBI StuI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII XmaCI XmaI XmnI