##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AgeI AhdI AscI
Asp700I Asp718I AspEI BamHI BbeI
BbrPI BciVI BlpI Bpu1102I BsiEI
BsiWI BsmBI BspDI BssHII BstBI
CelII ClaI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FseI
HpaI KasI KpnI KspI MfeI
MluI MunI NarI NdeI NotI
NruI NsiI NspV PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI SacII SalI SbfI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 859 TCCGGA T^CCGGA
Alw44I 5250 GTGCAC G^TGCAC
ApaI 4974 GGGCCC GGGCC^C
ApaLI 5250 GTGCAC G^TGCAC
AspI 6063 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5402 TGCGCA TGC^GCA
BbvCI 5607 CCTCAGC CCTCAGC(-5/-2)
BcgI 751 CGATAACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1684 TGATCA T^GATCA
BglII 4883 AGATCT A^GATCT
BsaI 3042 GAGACC GGTCTC(1/5)
BsaWI 859 TCCGGA [AT]^CCGG[AT]
BseAI 859 TCCGGA T^CCGGA
Bsp1407I 3608 TGTACA T^GTACA
BspEI 859 TCCGGA T^CCGGA
BsrBI 6168 CCGCTC CCGCTC(-3/-3)
BsrGI 3608 TGTACA T^GTACA
Bst1107I 5580 GTATAC GTA^TAC
BstAPI 1026 GCATCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4873 GGTCACC G^GT[ACGT]ACC
BstPI 4873 GGTCACC G^GT[ACGT]ACC
BstZ17I 5580 GTATAC GTA^TAC
CpoI 856 CGGTCCG CG^G[AT]CCG
DraIII 3529 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 6060 GACGACTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4873 GGTCACC G^GT[ACGT]ACC
FbaI 1684 TGATCA T^GATCA
FspI 5402 TGCGCA TGC^GCA
MroI 859 TCCGGA T^CCGGA
NaeI 5795 GCCGGC GCC^GGC
NgoMIV 5795 GCCGGC G^CCGGC
NheI 2091 GCTAGC G^CTAGC
PacI 4783 TTAATTAA TTAAT^TAA
PflFI 6063 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3714 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 5491 CTGCAG CTGCA^G
RsrII 856 CGGTCCG CG^G[AT]CCG
SacI 5653 GAGCTC GAGCT^C
SexAI 5584 ACCAGGT A^CC[AT]GGT
SpeI 4260 ACTAGT A^CTAGT
SspBI 3608 TGTACA T^GTACA
SwaI 47 ATTTAAAT ATTT^AAAT
Tth111I 6063 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3714 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 1765 GACGTC GACGT^C
3351 GACGTC
Aor51HI 4086 AGCGCT AGC^GCT
5523 AGCGCT
AvrII 144 CCTAGG C^CTAGG
3522 CCTAGG
BbsI 3804 GAAGAC GAAGAC(2/6)
5964 GAAGAC
BlnI 144 CCTAGG C^CTAGG
3522 CCTAGG
BpuAI 3804 GAAGAC GAAGAC(2/6)
5964 GAAGAC
BsaAI 2358 CACGTA [CT]AC^GT[GA]
6046 TACGTG
BsrFI 5190 ACCGGC [GA]^CCGG[CT]
5795 GCCGGC
BssSI 5700 CACGAG CACGAG(-5/-1)
4592 CTCGTG
BstXI 903 CCAAAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1436 CCATTTTCATGG
Cfr10I 5190 ACCGGC [GA]^CCGG[CT]
5795 GCCGGC
Eco47III 4086 AGCGCT AGC^GCT
5523 AGCGCT
EcoNI 2430 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6104 CCTTCTTCAGG
HaeII 4086 AGCGCT [GA]GCGC^[CT]
5523 AGCGCT
HincII 2806 GTCAAC GT[CT]^[GA]AC
4608 GTTGAC
HindII 2806 GTCAAC GT[CT]^[GA]AC
4608 GTTGAC
HindIII 1078 AAGCTT A^AGCTT
3006 AAGCTT
MspA1I 5488 CAGCTG C[AC]G^C[GT]G
5610 CAGCTG
PvuII 5488 CAGCTG CAG^CTG
5610 CAGCTG
SfiI 5637 GGCCATGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5798 GGCCCAGGCGGCC
SspI 65 AATATT AAT^ATT
3951 AATATT
StuI 586 AGGCCT AGG^CCT
5660 AGGCCT
TaqI 736 TCGA T^CGA
3454 TCGA
TthHB8I 736 TCGA T^CGA
3454 TCGA
XbaI 1174 TCTAGA T^CTAGA
4009 TCTAGA
XcmI 4415 CCATCCCAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5602 CCACACCTCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI AocI ApoI AseI AsnI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BfrI
BglI BmyI BpmI BsaBI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspLU11I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PshBI RcaI
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SphI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII