##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AclI AflIII Alw44I
ApaLI AscI Asp718I AspI AviII
BamHI BbrPI BsaAI BsaBI BsiWI
BsmI Bsp1407I BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstEII BstPI
BstXI BstZ17I CpoI EcoO65I EcoRV
FspI HpaI KpnI MamI MluI
NotI PacI PaeR7I PflFI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 5790 GTCTAC GT^[AC][GT]AC
AccIII 2614 TCCGGA T^CCGGA
AgeI 5819 ACCGGT A^CCGGT
AhdI 829 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3985 CCTCAGG CC^T[ACGT]AGG
AseI 5011 ATTAAT AT^TAAT
AsnI 5011 ATTAAT AT^TAAT
Asp700I 3379 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 829 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1825 CCTAGG C^CTAGG
BbeI 4133 GGCGCC GGCGC^C
BciVI 5393 GTATCC GTATCC(6/5)
BclI 764 TGATCA T^GATCA
BglII 5648 AGATCT A^GATCT
BlnI 1825 CCTAGG C^CTAGG
BlpI 819 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 819 GCTTAGC GC^T[ACGT]AGC
BseAI 2614 TCCGGA T^CCGGA
BsmBI 1929 CGTCTC CGTCTC(1/5)
BspDI 3769 ATCGAT AT^CGAT
BspEI 2614 TCCGGA T^CCGGA
BstAPI 1494 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 664 TTCGAA TT^CGAA
Bsu36I 3985 CCTCAGG CC^T[ACGT]AGG
CelII 819 GCTTAGC GC^T[ACGT]AGC
ClaI 3769 ATCGAT AT^CGAT
DraIII 2550 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 829 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3985 CCTCAGG CC^T[ACGT]AGG
EcoRI 4111 GAATTC G^AATTC
FbaI 764 TGATCA T^GATCA
FseI 1676 GGCCGGCC GGCCGG^CC
HincII 4026 GTCAAC GT[CT]^[GA]AC
HindII 4026 GTCAAC GT[CT]^[GA]AC
KasI 4133 GGCGCC G^GCGCC
MroI 2614 TCCGGA T^CCGGA
NarI 4133 GGCGCC GG^CGCC
NruI 498 TCGCGA TCG^CGA
NspV 664 TTCGAA TT^CGAA
PinAI 5819 ACCGGT A^CCGGT
PshAI 2683 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5011 ATTAAT AT^TAAT
PvuII 5520 CAGCTG CAG^CTG
SapI 606 GCTCTTC GCTCTTC(1/4)
SfoI 4133 GGCGCC GGC^GCC
SfuI 664 TTCGAA TT^CGAA
SspI 4358 AATATT AAT^ATT
XmnI 3379 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflII 641 CTTAAG C^TTAAG
1364 CTTAAG
BalI 3358 TGGCCA TGG^CCA
6159 TGGCCA
BcgI 3339 CGATGCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1788 GCATAATCATCG
BfrI 641 CTTAAG C^TTAAG
1364 CTTAAG
BsaI 3455 GAGACC GGTCTC(1/5)
2101 GGTCTC
BssHII 305 GCGCGC G^CGCGC
1611 GCGCGC
DrdI 468 GACCCGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3220 GACTCTCACGTC
KspI 389 CCGCGG CCGC^GG
4155 CCGCGG
MluNI 3358 TGGCCA TGG^CCA
6159 TGGCCA
MscI 3358 TGGCCA TGG^CCA
6159 TGGCCA
NcoI 3490 CCATGG C^CATGG
5914 CCATGG
NdeI 1746 CATATG CA^TATG
6033 CATATG
NheI 4073 GCTAGC G^CTAGC
5298 GCTAGC
SacI 1217 GAGCTC GAGCT^C
1560 GAGCTC
SacII 389 CCGCGG CCGC^GG
4155 CCGCGG
SphI 411 GCATGC GCATG^C
5974 GCATGC
XbaI 1213 TCTAGA T^CTAGA
2012 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI