##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AviII BcgI BciVI
BglI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BsrBI BssHII BstAPI CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I FseI FspI HincII
HindII HpaI KpnI KspI MamI
MluI MroI NdeI NotI NruI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SmaI SpeI SphI
Sse8387I SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 4057 GTATAC GT^[AC][GT]AC
AgeI 132 ACCGGT A^CCGGT
Aor51HI 54 AGCGCT AGC^GCT
AspI 268 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1753 TGGCCA TGG^CCA
BbeI 122 GGCGCC GGCGC^C
BclI 5290 TGATCA T^GATCA
BsaWI 132 ACCGGT [AT]^CCGG[AT]
BsmI 4404 GAATGC GAATGC(1/-1)
Bsp1407I 4892 TGTACA T^GTACA
BsrDI 4111 GCAATG GCAATG(2/0)
BsrGI 4892 TGTACA T^GTACA
BssSI 1332 CACGAG CACGAG(-5/-1)
Bst1107I 4057 GTATAC GTA^TAC
BstEII 823 GGTCACC G^GT[ACGT]ACC
BstPI 823 GGTCACC G^GT[ACGT]ACC
BstZ17I 4057 GTATAC GTA^TAC
EaeI 1753 TGGCCA [CT]^GGCC[GA]
Eco47III 54 AGCGCT AGC^GCT
EcoO65I 823 GGTCACC G^GT[ACGT]ACC
EcoRI 3945 GAATTC G^AATTC
EcoRV 698 GATATC GAT^ATC
FbaI 5290 TGATCA T^GATCA
KasI 122 GGCGCC G^GCGCC
MfeI 942 CAATTG C^AATTG
MluNI 1753 TGGCCA TGG^CCA
MscI 1753 TGGCCA TGG^CCA
MunI 942 CAATTG C^AATTG
NarI 122 GGCGCC GG^CGCC
NheI 5030 GCTAGC G^CTAGC
PacI 3601 TTAATTAA TTAAT^TAA
PaeR7I 830 CTCGAG C^TCGAG
PflFI 268 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1846 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 132 ACCGGT A^CCGGT
PshAI 3264 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1159 GAGCTC GAGCT^C
SapI 2158 GAAGAGC GCTCTTC(1/4)
SfoI 122 GGCGCC GGC^GCC
SgrAI 295 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 4060 TACGTA TAC^GTA
SspBI 4892 TGTACA T^GTACA
StuI 834 AGGCCT AGG^CCT
Tth111I 268 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1846 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2780 TCTAGA T^CTAGA
XcmI 966 CCAACGCTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 830 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 122 GGCGCC G[GA]^CG[CT]C
782 GACGCC
AocI 928 CCTGAGG CC^T[ACGT]AGG
2840 CCTTAGG
Asp700I 2158 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4025 GAAAGTCTTC
AvrII 187 CCTAGG C^CTAGG
5160 CCTAGG
BamHI 1495 GGATCC G^GATCC
3067 GGATCC
BanI 122 GGCGCC G^G[CT][GA]CC
1811 GGTGCC
BbrPI 4436 CACGTG CAC^GTG
5219 CACGTG
BlnI 187 CCTAGG C^CTAGG
5160 CCTAGG
BsaHI 122 GGCGCC G[GA]^CG[CT]C
782 GACGCC
BsmBI 1335 GAGACG CGTCTC(1/5)
1838 GAGACG
BspLU11I 3104 ACATGT A^CATGT
5008 ACATGT
BspMI 5026 ACCTGC ACCTGC(4/8)
1301 GCAGGT
BstBI 2418 TTCGAA TT^CGAA
4843 TTCGAA
Bsu36I 928 CCTGAGG CC^T[ACGT]AGG
2840 CCTTAGG
Eco81I 928 CCTGAGG CC^T[ACGT]AGG
2840 CCTTAGG
EcoNI 1046 CCTTTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2651 CCTTATCCAGG
HgaI 26 GACGC GACGC(5/10)
782 GACGC
Hin1I 122 GGCGCC G[GA]^CG[CT]C
782 GACGCC
MspA1I 1806 CAGCTG C[AC]G^C[GT]G
1977 CAGCTG
NcoI 1491 CCATGG C^CATGG
2178 CCATGG
NspV 2418 TTCGAA TT^CGAA
4843 TTCGAA
PmaCI 4436 CACGTG CAC^GTG
5219 CACGTG
PmlI 4436 CACGTG CAC^GTG
5219 CACGTG
PvuII 1806 CAGCTG CAG^CTG
1977 CAGCTG
SfuI 2418 TTCGAA TT^CGAA
4843 TTCGAA
XmnI 2158 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4025 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BanII
BbsI BbvCI BbvI BcnI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NsiI NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII