##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI AspEI BbrPI
BglII BsaWI BseAI BsiWI BspDI
BspEI BssHII BstBI BstEII BstPI
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV FseI
KspI MfeI MroI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SapI
SbfI SfiI SfuI SgrAI SnaBI
SphI Sse8387I SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 354 GACGTC GACGT^C
Acc65I 3437 GGTACC G^GTACC
AflII 5171 CTTAAG C^TTAAG
Asp718I 3437 GGTACC G^GTACC
AviII 851 TGCGCA TGC^GCA
BamHI 5545 GGATCC G^GATCC
BbeI 290 GGCGCC GGCGC^C
BfrI 5171 CTTAAG C^TTAAG
BsaAI 685 TACGTG [CT]AC^GT[GA]
BsiEI 741 CGACCG CG[GA][CT]^CG
BsmBI 1366 GAGACG CGTCTC(1/5)
BsmI 4234 GAATGC GAATGC(1/-1)
BspHI 749 TCATGA T^CATGA
BsrFI 794 GCCGGC [GA]^CCGG[CT]
Bst1107I 1137 GTATAC GTA^TAC
BstAPI 897 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1137 GTATAC GTA^TAC
Cfr10I 794 GCCGGC [GA]^CCGG[CT]
DraIII 898 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 402 GACATCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2803 ATGCAT ATGCA^T
FspI 851 TGCGCA TGC^GCA
HpaI 4700 GTTAAC GTT^AAC
KasI 290 GGCGCC G^GCGCC
KpnI 3437 GGTACC GGTAC^C
MluI 2055 ACGCGT A^CGCGT
NaeI 794 GCCGGC GCC^GGC
NarI 290 GGCGCC GG^CGCC
NcoI 4385 CCATGG C^CATGG
NgoMIV 794 GCCGGC G^CCGGC
NsiI 2803 ATGCAT ATGCA^T
RcaI 749 TCATGA T^CATGA
SacI 4254 GAGCTC GAGCT^C
SalI 2552 GTCGAC G^TCGAC
ScaI 4295 AGTACT AGT^ACT
SexAI 899 ACCTGGT A^CC[AT]GGT
SfoI 290 GGCGCC GGC^GCC
SpeI 2795 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AclI 3016 AACGTT AA^CGTT
5135 AACGTT
Aor51HI 396 AGCGCT AGC^GCT
878 AGCGCT
ApaI 618 GGGCCC GGGCC^C
1313 GGGCCC
AspI 3452 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4219 GACCTTGTC
AvrII 2776 CCTAGG C^CTAGG
5011 CCTAGG
BcgI 4975 CGAGGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1465 GCAGGAGAATCG
BlnI 2776 CCTAGG C^CTAGG
5011 CCTAGG
BlpI 4379 GCTGAGC GC^T[ACGT]AGC
4902 GCTCAGC
Bpu1102I 4379 GCTGAGC GC^T[ACGT]AGC
4902 GCTCAGC
Bsp1407I 1163 TGTACA T^GTACA
5637 TGTACA
BsrDI 4552 CATTGC GCAATG(2/0)
369 GCAATG
BsrGI 1163 TGTACA T^GTACA
5637 TGTACA
BssSI 233 CACGAG CACGAG(-5/-1)
5096 CACGAG
BstYI 5469 AGATCC [GA]^GATC[CT]
5545 GGATCC
CelII 4379 GCTGAGC GC^T[ACGT]AGC
4902 GCTCAGC
DraI 2422 TTTAAA TTT^AAA
3710 TTTAAA
EarI 2066 CTCTTC CTCTTC(1/4)
2286 GAAGAG
Eco47III 396 AGCGCT AGC^GCT
878 AGCGCT
EcoNI 1611 CCTTTAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2759 CCTAGTGCAGG
HincII 2552 GTCGAC GT[CT]^[GA]AC
4700 GTTAAC
HindII 2552 GTCGAC GT[CT]^[GA]AC
4700 GTTAAC
Ksp632I 2066 CTCTTC CTCTTC(1/4)
2286 GAAGAG
MflI 5469 AGATCC [GA]^GATC[CT]
5545 GGATCC
PflFI 3452 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4219 GACCTTGTC
Psp1406I 3016 AACGTT AA^CGTT
5135 AACGTT
SspBI 1163 TGTACA T^GTACA
5637 TGTACA
Tth111I 3452 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4219 GACCTTGTC
XhoII 5469 AGATCC [GA]^GATC[CT]
5545 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BciVI BclI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MamI
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XmaCI XmaI
XmnI