################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AccIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI BbrPI BglII BsaWI BseAI BsiWI BspDI BspEI BssHII BstBI BstEII BstPI ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV FseI KspI MfeI MroI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SapI SbfI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 354 GACGTC GACGT^C Acc65I 3437 GGTACC G^GTACC AflII 5171 CTTAAG C^TTAAG Asp718I 3437 GGTACC G^GTACC AviII 851 TGCGCA TGC^GCA BamHI 5545 GGATCC G^GATCC BbeI 290 GGCGCC GGCGC^C BfrI 5171 CTTAAG C^TTAAG BsaAI 685 TACGTG [CT]AC^GT[GA] BsiEI 741 CGACCG CG[GA][CT]^CG BsmBI 1366 GAGACG CGTCTC(1/5) BsmI 4234 GAATGC GAATGC(1/-1) BspHI 749 TCATGA T^CATGA BsrFI 794 GCCGGC [GA]^CCGG[CT] Bst1107I 1137 GTATAC GTA^TAC BstAPI 897 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 1137 GTATAC GTA^TAC Cfr10I 794 GCCGGC [GA]^CCGG[CT] DraIII 898 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 402 GACATCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 2803 ATGCAT ATGCA^T FspI 851 TGCGCA TGC^GCA HpaI 4700 GTTAAC GTT^AAC KasI 290 GGCGCC G^GCGCC KpnI 3437 GGTACC GGTAC^C MluI 2055 ACGCGT A^CGCGT NaeI 794 GCCGGC GCC^GGC NarI 290 GGCGCC GG^CGCC NcoI 4385 CCATGG C^CATGG NgoMIV 794 GCCGGC G^CCGGC NsiI 2803 ATGCAT ATGCA^T RcaI 749 TCATGA T^CATGA SacI 4254 GAGCTC GAGCT^C SalI 2552 GTCGAC G^TCGAC ScaI 4295 AGTACT AGT^ACT SexAI 899 ACCTGGT A^CC[AT]GGT SfoI 290 GGCGCC GGC^GCC SpeI 2795 ACTAGT A^CTAGT ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AclI 3016 AACGTT AA^CGTT 5135 AACGTT Aor51HI 396 AGCGCT AGC^GCT 878 AGCGCT ApaI 618 GGGCCC GGGCC^C 1313 GGGCCC AspI 3452 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4219 GACCTTGTC AvrII 2776 CCTAGG C^CTAGG 5011 CCTAGG BcgI 4975 CGAGGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1465 GCAGGAGAATCG BlnI 2776 CCTAGG C^CTAGG 5011 CCTAGG BlpI 4379 GCTGAGC GC^T[ACGT]AGC 4902 GCTCAGC Bpu1102I 4379 GCTGAGC GC^T[ACGT]AGC 4902 GCTCAGC Bsp1407I 1163 TGTACA T^GTACA 5637 TGTACA BsrDI 4552 CATTGC GCAATG(2/0) 369 GCAATG BsrGI 1163 TGTACA T^GTACA 5637 TGTACA BssSI 233 CACGAG CACGAG(-5/-1) 5096 CACGAG BstYI 5469 AGATCC [GA]^GATC[CT] 5545 GGATCC CelII 4379 GCTGAGC GC^T[ACGT]AGC 4902 GCTCAGC DraI 2422 TTTAAA TTT^AAA 3710 TTTAAA EarI 2066 CTCTTC CTCTTC(1/4) 2286 GAAGAG Eco47III 396 AGCGCT AGC^GCT 878 AGCGCT EcoNI 1611 CCTTTAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2759 CCTAGTGCAGG HincII 2552 GTCGAC GT[CT]^[GA]AC 4700 GTTAAC HindII 2552 GTCGAC GT[CT]^[GA]AC 4700 GTTAAC Ksp632I 2066 CTCTTC CTCTTC(1/4) 2286 GAAGAG MflI 5469 AGATCC [GA]^GATC[CT] 5545 GGATCC PflFI 3452 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4219 GACCTTGTC Psp1406I 3016 AACGTT AA^CGTT 5135 AACGTT SspBI 1163 TGTACA T^GTACA 5637 TGTACA Tth111I 3452 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4219 GACCTTGTC XhoII 5469 AGATCC [GA]^GATC[CT] 5545 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BciVI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MamI MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XmaCI XmaI XmnI