################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AatII AcyI Alw44I Aor51HI ApaLI AscI AseI AsnI AviII AvrII BbeI BbrPI BbvCI BcgI BlnI BsaHI BsiWI BspLU11I BssHII CpoI DrdI Eco47III FseI FspI Hin1I KasI KspI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SalI SbfI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 3715 GGTACC G^GTACC AclI 3099 AACGTT AA^CGTT AflII 900 CTTAAG C^TTAAG AgeI 849 ACCGGT A^CCGGT Asp700I 1533 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3715 GGTACC G^GTACC BclI 673 TGATCA T^GATCA BfrI 900 CTTAAG C^TTAAG BglII 4861 AGATCT A^GATCT BlpI 4050 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4050 GCTCAGC GC^T[ACGT]AGC BsaBI 3086 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 5029 GAGACG CGTCTC(1/5) BspDI 1854 ATCGAT AT^CGAT BspHI 2377 TCATGA T^CATGA BssSI 528 CACGAG CACGAG(-5/-1) Bst1107I 731 GTATAC GTA^TAC BstBI 2980 TTCGAA TT^CGAA BstZ17I 731 GTATAC GTA^TAC CelII 4050 GCTCAGC GC^T[ACGT]AGC ClaI 1854 ATCGAT AT^CGAT DraI 2429 TTTAAA TTT^AAA EagI 5008 CGGCCG C^GGCCG EclXI 5008 CGGCCG C^GGCCG Eco52I 5008 CGGCCG C^GGCCG EcoRI 61 GAATTC G^AATTC EcoRV 122 GATATC GAT^ATC EcoT22I 306 ATGCAT ATGCA^T FbaI 673 TGATCA T^GATCA HindIII 4707 AAGCTT A^AGCTT HpaI 3527 GTTAAC GTT^AAC KpnI 3715 GGTACC GGTAC^C MamI 3086 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 5664 CAATTG C^AATTG MunI 5664 CAATTG C^AATTG NsiI 306 ATGCAT ATGCA^T NspV 2980 TTCGAA TT^CGAA PaeR7I 2794 CTCGAG C^TCGAG PflMI 5336 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 849 ACCGGT A^CCGGT PshAI 2538 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3099 AACGTT AA^CGTT RcaI 2377 TCATGA T^CATGA SapI 576 GAAGAGC GCTCTTC(1/4) ScaI 2848 AGTACT AGT^ACT SfuI 2980 TTCGAA TT^CGAA SmaI 2200 CCCGGG CCC^GGG SwaI 2428 ATTTAAAT ATTT^AAAT Van91I 5336 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1955 TCTAGA T^CTAGA XhoI 2794 CTCGAG C^TCGAG XmaCI 2200 CCCGGG C^CCGGG XmaI 2200 CCCGGG C^CCGGG XmnI 1533 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 731 GTATAC GT^[AC][GT]AC 2655 GTCTAC AhdI 507 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5701 GACATTCTGTC AspEI 507 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5701 GACATTCTGTC AspI 3617 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4440 GACGAAGTC BalI 2113 TGGCCA TGG^CCA 5294 TGGCCA BamHI 2011 GGATCC G^GATCC 2974 GGATCC BciVI 1627 GTATCC GTATCC(6/5) 3750 GTATCC BsaAI 3386 TACGTG [CT]AC^GT[GA] 4715 TACGTG BsiEI 237 CGACCG CG[GA][CT]^CG 5008 CGGCCG Bsp1407I 4743 TGTACA T^GTACA 4841 TGTACA BsrFI 849 ACCGGT [GA]^CCGG[CT] 3941 GCCGGT BsrGI 4743 TGTACA T^GTACA 4841 TGTACA BstAPI 2597 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5504 GCAGCTCTTGC BstEII 1358 GGTGACC G^GT[ACGT]ACC 3689 GGTGACC BstPI 1358 GGTGACC G^GT[ACGT]ACC 3689 GGTGACC Cfr10I 849 ACCGGT [GA]^CCGG[CT] 3941 GCCGGT Eam1105I 507 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5701 GACATTCTGTC EcoO65I 1358 GGTGACC G^GT[ACGT]ACC 3689 GGTGACC HaeII 2179 AGCGCC [GA]GCGC^[CT] 5059 AGCGCC MluNI 2113 TGGCCA TGG^CCA 5294 TGGCCA MscI 2113 TGGCCA TGG^CCA 5294 TGGCCA NdeI 1374 CATATG CA^TATG 2841 CATATG NspI 5683 GCATGT [GA]CATG^[CT] 5718 GCATGT PflFI 3617 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4440 GACGAAGTC SexAI 2350 ACCTGGT A^CC[AT]GGT 2742 ACCTGGT SfiI 467 GGCCCGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1997 GGCCCAGCAGGCC SspBI 4743 TGTACA T^GTACA 4841 TGTACA Tth111I 3617 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4440 GACGAAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AccIII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII