##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AcyI Alw44I Aor51HI ApaLI
AscI AseI AsnI AviII AvrII
BbeI BbrPI BbvCI BcgI BlnI
BsaHI BsiWI BspLU11I BssHII CpoI
DrdI Eco47III FseI FspI Hin1I
KasI KspI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacII SalI SbfI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 3715 GGTACC G^GTACC
AclI 3099 AACGTT AA^CGTT
AflII 900 CTTAAG C^TTAAG
AgeI 849 ACCGGT A^CCGGT
Asp700I 1533 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3715 GGTACC G^GTACC
BclI 673 TGATCA T^GATCA
BfrI 900 CTTAAG C^TTAAG
BglII 4861 AGATCT A^GATCT
BlpI 4050 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4050 GCTCAGC GC^T[ACGT]AGC
BsaBI 3086 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 5029 GAGACG CGTCTC(1/5)
BspDI 1854 ATCGAT AT^CGAT
BspHI 2377 TCATGA T^CATGA
BssSI 528 CACGAG CACGAG(-5/-1)
Bst1107I 731 GTATAC GTA^TAC
BstBI 2980 TTCGAA TT^CGAA
BstZ17I 731 GTATAC GTA^TAC
CelII 4050 GCTCAGC GC^T[ACGT]AGC
ClaI 1854 ATCGAT AT^CGAT
DraI 2429 TTTAAA TTT^AAA
EagI 5008 CGGCCG C^GGCCG
EclXI 5008 CGGCCG C^GGCCG
Eco52I 5008 CGGCCG C^GGCCG
EcoRI 61 GAATTC G^AATTC
EcoRV 122 GATATC GAT^ATC
EcoT22I 306 ATGCAT ATGCA^T
FbaI 673 TGATCA T^GATCA
HindIII 4707 AAGCTT A^AGCTT
HpaI 3527 GTTAAC GTT^AAC
KpnI 3715 GGTACC GGTAC^C
MamI 3086 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5664 CAATTG C^AATTG
MunI 5664 CAATTG C^AATTG
NsiI 306 ATGCAT ATGCA^T
NspV 2980 TTCGAA TT^CGAA
PaeR7I 2794 CTCGAG C^TCGAG
PflMI 5336 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 849 ACCGGT A^CCGGT
PshAI 2538 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3099 AACGTT AA^CGTT
RcaI 2377 TCATGA T^CATGA
SapI 576 GAAGAGC GCTCTTC(1/4)
ScaI 2848 AGTACT AGT^ACT
SfuI 2980 TTCGAA TT^CGAA
SmaI 2200 CCCGGG CCC^GGG
SwaI 2428 ATTTAAAT ATTT^AAAT
Van91I 5336 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1955 TCTAGA T^CTAGA
XhoI 2794 CTCGAG C^TCGAG
XmaCI 2200 CCCGGG C^CCGGG
XmaI 2200 CCCGGG C^CCGGG
XmnI 1533 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 731 GTATAC GT^[AC][GT]AC
2655 GTCTAC
AhdI 507 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5701 GACATTCTGTC
AspEI 507 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5701 GACATTCTGTC
AspI 3617 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4440 GACGAAGTC
BalI 2113 TGGCCA TGG^CCA
5294 TGGCCA
BamHI 2011 GGATCC G^GATCC
2974 GGATCC
BciVI 1627 GTATCC GTATCC(6/5)
3750 GTATCC
BsaAI 3386 TACGTG [CT]AC^GT[GA]
4715 TACGTG
BsiEI 237 CGACCG CG[GA][CT]^CG
5008 CGGCCG
Bsp1407I 4743 TGTACA T^GTACA
4841 TGTACA
BsrFI 849 ACCGGT [GA]^CCGG[CT]
3941 GCCGGT
BsrGI 4743 TGTACA T^GTACA
4841 TGTACA
BstAPI 2597 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5504 GCAGCTCTTGC
BstEII 1358 GGTGACC G^GT[ACGT]ACC
3689 GGTGACC
BstPI 1358 GGTGACC G^GT[ACGT]ACC
3689 GGTGACC
Cfr10I 849 ACCGGT [GA]^CCGG[CT]
3941 GCCGGT
Eam1105I 507 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5701 GACATTCTGTC
EcoO65I 1358 GGTGACC G^GT[ACGT]ACC
3689 GGTGACC
HaeII 2179 AGCGCC [GA]GCGC^[CT]
5059 AGCGCC
MluNI 2113 TGGCCA TGG^CCA
5294 TGGCCA
MscI 2113 TGGCCA TGG^CCA
5294 TGGCCA
NdeI 1374 CATATG CA^TATG
2841 CATATG
NspI 5683 GCATGT [GA]CATG^[CT]
5718 GCATGT
PflFI 3617 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4440 GACGAAGTC
SexAI 2350 ACCTGGT A^CC[AT]GGT
2742 ACCTGGT
SfiI 467 GGCCCGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1997 GGCCCAGCAGGCC
SspBI 4743 TGTACA T^GTACA
4841 TGTACA
Tth111I 3617 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4440 GACGAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII