##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

AatII       AcyI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        AviII       AvrII       
BbeI        BbrPI       BbvCI       BcgI        BlnI        
BsaHI       BsiWI       BspLU11I    BssHII      CpoI        
DrdI        Eco47III    FseI        FspI        Hin1I       
KasI        KspI        MluI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        PacI        
PmaCI       PmeI        PmlI        PshBI       PvuI        
RsrII       SacII       SalI        SbfI        SfoI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I       3715  GGTACC               G^GTACC
AclI         3099  AACGTT               AA^CGTT
AflII         900  CTTAAG               C^TTAAG
AgeI          849  ACCGGT               A^CCGGT
Asp700I      1533  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3715  GGTACC               G^GTACC
BclI          673  TGATCA               T^GATCA
BfrI          900  CTTAAG               C^TTAAG
BglII        4861  AGATCT               A^GATCT
BlpI         4050  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     4050  GCTCAGC              GC^T[ACGT]AGC
BsaBI        3086  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        5029  GAGACG               CGTCTC(1/5)
BspDI        1854  ATCGAT               AT^CGAT
BspHI        2377  TCATGA               T^CATGA
BssSI         528  CACGAG               CACGAG(-5/-1)
Bst1107I      731  GTATAC               GTA^TAC
BstBI        2980  TTCGAA               TT^CGAA
BstZ17I       731  GTATAC               GTA^TAC
CelII        4050  GCTCAGC              GC^T[ACGT]AGC
ClaI         1854  ATCGAT               AT^CGAT
DraI         2429  TTTAAA               TTT^AAA
EagI         5008  CGGCCG               C^GGCCG
EclXI        5008  CGGCCG               C^GGCCG
Eco52I       5008  CGGCCG               C^GGCCG
EcoRI          61  GAATTC               G^AATTC
EcoRV         122  GATATC               GAT^ATC
EcoT22I       306  ATGCAT               ATGCA^T
FbaI          673  TGATCA               T^GATCA
HindIII      4707  AAGCTT               A^AGCTT
HpaI         3527  GTTAAC               GTT^AAC
KpnI         3715  GGTACC               GGTAC^C
MamI         3086  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         5664  CAATTG               C^AATTG
MunI         5664  CAATTG               C^AATTG
NsiI          306  ATGCAT               ATGCA^T
NspV         2980  TTCGAA               TT^CGAA
PaeR7I       2794  CTCGAG               C^TCGAG
PflMI        5336  CCAAGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         849  ACCGGT               A^CCGGT
PshAI        2538  GACAAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3099  AACGTT               AA^CGTT
RcaI         2377  TCATGA               T^CATGA
SapI          576  GAAGAGC              GCTCTTC(1/4)
ScaI         2848  AGTACT               AGT^ACT
SfuI         2980  TTCGAA               TT^CGAA
SmaI         2200  CCCGGG               CCC^GGG
SwaI         2428  ATTTAAAT             ATTT^AAAT
Van91I       5336  CCAAGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1955  TCTAGA               T^CTAGA
XhoI         2794  CTCGAG               C^TCGAG
XmaCI        2200  CCCGGG               C^CCGGG
XmaI         2200  CCCGGG               C^CCGGG
XmnI         1533  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          731  GTATAC               GT^[AC][GT]AC
             2655  GTCTAC              
AhdI          507  GACGTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5701  GACATTCTGTC         
AspEI         507  GACGTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5701  GACATTCTGTC         
AspI         3617  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4440  GACGAAGTC           
BalI         2113  TGGCCA               TGG^CCA
             5294  TGGCCA              
BamHI        2011  GGATCC               G^GATCC
             2974  GGATCC              
BciVI        1627  GTATCC               GTATCC(6/5)
             3750  GTATCC              
BsaAI        3386  TACGTG               [CT]AC^GT[GA]
             4715  TACGTG              
BsiEI         237  CGACCG               CG[GA][CT]^CG
             5008  CGGCCG              
Bsp1407I     4743  TGTACA               T^GTACA
             4841  TGTACA              
BsrFI         849  ACCGGT               [GA]^CCGG[CT]
             3941  GCCGGT              
BsrGI        4743  TGTACA               T^GTACA
             4841  TGTACA              
BstAPI       2597  GCAGCTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             5504  GCAGCTCTTGC         
BstEII       1358  GGTGACC              G^GT[ACGT]ACC
             3689  GGTGACC             
BstPI        1358  GGTGACC              G^GT[ACGT]ACC
             3689  GGTGACC             
Cfr10I        849  ACCGGT               [GA]^CCGG[CT]
             3941  GCCGGT              
Eam1105I      507  GACGTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5701  GACATTCTGTC         
EcoO65I      1358  GGTGACC              G^GT[ACGT]ACC
             3689  GGTGACC             
HaeII        2179  AGCGCC               [GA]GCGC^[CT]
             5059  AGCGCC              
MluNI        2113  TGGCCA               TGG^CCA
             5294  TGGCCA              
MscI         2113  TGGCCA               TGG^CCA
             5294  TGGCCA              
NdeI         1374  CATATG               CA^TATG
             2841  CATATG              
NspI         5683  GCATGT               [GA]CATG^[CT]
             5718  GCATGT              
PflFI        3617  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4440  GACGAAGTC           
SexAI        2350  ACCTGGT              A^CC[AT]GGT
             2742  ACCTGGT             
SfiI          467  GGCCCGTGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1997  GGCCCAGCAGGCC       
SspBI        4743  TGTACA               T^GTACA
             4841  TGTACA              
Tth111I      3617  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4440  GACGAAGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccII       AccIII      AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApaI        ApoI        AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspEI       
BspMI       BsrBI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MroI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII