##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AccI        AccII       AccIII      AclI        AflII       
AgeI        AscI        AseI        AsnI        AspI        
AvrII       BamHI       BcgI        BciVI       BfrI        
BlnI        BsaBI       BseAI       BsiWI       BsmI        
BspDI       BspEI       BssHII      Bst1107I    BstBI       
BstUI       BstZ17I     ClaI        CpoI        EcoRI       
EcoRV       FseI        KspI        MamI        MluI        
MroI        MvnI        NdeI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
SfiI        SfuI        SnaBI       Sse8387I    SspI        
SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        1463  GACGTC               GACGT^C
AcsI         3820  GAATTT               [GA]^AATT[CT]
Aor51HI      2334  AGCGCT               AGC^GCT
ApoI         3820  GAATTT               [GA]^AATT[CT]
AviII        4731  TGCGCA               TGC^GCA
BbeI         3275  GGCGCC               GGCGC^C
BbrPI        4198  CACGTG               CAC^GTG
BbvCI        3729  GCTGAGG              CCTCAGC(-5/-2)
BclI         2010  TGATCA               T^GATCA
BsaWI        2244  TCCGGT               [AT]^CCGG[AT]
BsiEI        2967  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1224  TGTACA               T^GTACA
BspLU11I     4092  ACATGT               A^CATGT
BsrGI        1224  TGTACA               T^GTACA
DraI         5560  TTTAAA               TTT^AAA
DrdI         3683  GACTAGTGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2967  CGGCCG               C^GGCCG
EclXI        2967  CGGCCG               C^GGCCG
Eco47III     2334  AGCGCT               AGC^GCT
Eco52I       2967  CGGCCG               C^GGCCG
EcoT22I      5388  ATGCAT               ATGCA^T
FbaI         2010  TGATCA               T^GATCA
FspI         4731  TGCGCA               TGC^GCA
HpaI         5346  GTTAAC               GTT^AAC
KasI         3275  GGCGCC               G^GCGCC
MfeI           20  CAATTG               C^AATTG
MunI           20  CAATTG               C^AATTG
NarI         3275  GGCGCC               GG^CGCC
NsiI         5388  ATGCAT               ATGCA^T
PmaCI        4198  CACGTG               CAC^GTG
PmlI         4198  CACGTG               CAC^GTG
PshAI        3850  GACTGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI          166  AGTACT               AGT^ACT
SfoI         3275  GGCGCC               GGC^GCC
SgrAI        2299  CACCGGCG             C[GA]^CCGG[CT]G
SspBI        1224  TGTACA               T^GTACA
StuI         4032  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Acc65I        128  GGTACC               G^GTACC
              582  GGTACC              
Asp718I       128  GGTACC               G^GTACC
              582  GGTACC              
BlpI          622  GCTGAGC              GC^T[ACGT]AGC
             2744  GCTGAGC             
Bpu1102I      622  GCTGAGC              GC^T[ACGT]AGC
             2744  GCTGAGC             
BsaI         2247  GGTCTC               GGTCTC(1/5)
             4987  GGTCTC              
BsmBI         757  CGTCTC               CGTCTC(1/5)
             3807  CGTCTC              
BspHI        1068  TCATGA               T^CATGA
             1152  TCATGA              
BssSI        3004  CACGAG               CACGAG(-5/-1)
             5093  CTCGTG              
BstEII       2524  GGTGACC              G^GT[ACGT]ACC
             2665  GGTCACC             
BstPI        2524  GGTGACC              G^GT[ACGT]ACC
             2665  GGTCACC             
CelII         622  GCTGAGC              GC^T[ACGT]AGC
             2744  GCTGAGC             
EcoNI        1534  CCTGTCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5106  CCTCGCACAGG         
EcoO65I      2524  GGTGACC              G^GT[ACGT]ACC
             2665  GGTCACC             
HincII        557  GTCAAC               GT[CT]^[GA]AC
             5346  GTTAAC              
HindII        557  GTCAAC               GT[CT]^[GA]AC
             5346  GTTAAC              
HindIII       190  AAGCTT               A^AGCTT
             4379  AAGCTT              
KpnI          128  GGTACC               GGTAC^C
              582  GGTACC              
PflMI        1407  CCATCAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4249  CCAGCCTTTGG         
RcaI         1068  TCATGA               T^CATGA
             1152  TCATGA              
SacI         3710  GAGCTC               GAGCT^C
             3907  GAGCTC              
SexAI        1092  ACCTGGT              A^CC[AT]GGT
             2520  ACCAGGT             
SmlI         4382  CTTGAG               C^T[CT][GA]AG
             4389  CTCAAG              
SpeI         3684  ACTAGT               A^CTAGT
             5253  ACTAGT              
Van91I       1407  CCATCAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4249  CCAGCCTTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  133 enzymes) #
#########################################

AciI        AcyI        AfaI        AflIII      AhdI        
AluI        Alw44I      AlwI        AlwNI       AocI        
ApaI        ApaLI       Asp700I     AspEI       AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BglI        
BglII       BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        Eam1105I    EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SphI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
XmaCI       XmaI        XmnI