##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccI AccII AccIII AclI AflII
AgeI AscI AseI AsnI AspI
AvrII BamHI BcgI BciVI BfrI
BlnI BsaBI BseAI BsiWI BsmI
BspDI BspEI BssHII Bst1107I BstBI
BstUI BstZ17I ClaI CpoI EcoRI
EcoRV FseI KspI MamI MluI
MroI MvnI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SfuI SnaBI Sse8387I SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1463 GACGTC GACGT^C
AcsI 3820 GAATTT [GA]^AATT[CT]
Aor51HI 2334 AGCGCT AGC^GCT
ApoI 3820 GAATTT [GA]^AATT[CT]
AviII 4731 TGCGCA TGC^GCA
BbeI 3275 GGCGCC GGCGC^C
BbrPI 4198 CACGTG CAC^GTG
BbvCI 3729 GCTGAGG CCTCAGC(-5/-2)
BclI 2010 TGATCA T^GATCA
BsaWI 2244 TCCGGT [AT]^CCGG[AT]
BsiEI 2967 CGGCCG CG[GA][CT]^CG
Bsp1407I 1224 TGTACA T^GTACA
BspLU11I 4092 ACATGT A^CATGT
BsrGI 1224 TGTACA T^GTACA
DraI 5560 TTTAAA TTT^AAA
DrdI 3683 GACTAGTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2967 CGGCCG C^GGCCG
EclXI 2967 CGGCCG C^GGCCG
Eco47III 2334 AGCGCT AGC^GCT
Eco52I 2967 CGGCCG C^GGCCG
EcoT22I 5388 ATGCAT ATGCA^T
FbaI 2010 TGATCA T^GATCA
FspI 4731 TGCGCA TGC^GCA
HpaI 5346 GTTAAC GTT^AAC
KasI 3275 GGCGCC G^GCGCC
MfeI 20 CAATTG C^AATTG
MunI 20 CAATTG C^AATTG
NarI 3275 GGCGCC GG^CGCC
NsiI 5388 ATGCAT ATGCA^T
PmaCI 4198 CACGTG CAC^GTG
PmlI 4198 CACGTG CAC^GTG
PshAI 3850 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 166 AGTACT AGT^ACT
SfoI 3275 GGCGCC GGC^GCC
SgrAI 2299 CACCGGCG C[GA]^CCGG[CT]G
SspBI 1224 TGTACA T^GTACA
StuI 4032 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 128 GGTACC G^GTACC
582 GGTACC
Asp718I 128 GGTACC G^GTACC
582 GGTACC
BlpI 622 GCTGAGC GC^T[ACGT]AGC
2744 GCTGAGC
Bpu1102I 622 GCTGAGC GC^T[ACGT]AGC
2744 GCTGAGC
BsaI 2247 GGTCTC GGTCTC(1/5)
4987 GGTCTC
BsmBI 757 CGTCTC CGTCTC(1/5)
3807 CGTCTC
BspHI 1068 TCATGA T^CATGA
1152 TCATGA
BssSI 3004 CACGAG CACGAG(-5/-1)
5093 CTCGTG
BstEII 2524 GGTGACC G^GT[ACGT]ACC
2665 GGTCACC
BstPI 2524 GGTGACC G^GT[ACGT]ACC
2665 GGTCACC
CelII 622 GCTGAGC GC^T[ACGT]AGC
2744 GCTGAGC
EcoNI 1534 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5106 CCTCGCACAGG
EcoO65I 2524 GGTGACC G^GT[ACGT]ACC
2665 GGTCACC
HincII 557 GTCAAC GT[CT]^[GA]AC
5346 GTTAAC
HindII 557 GTCAAC GT[CT]^[GA]AC
5346 GTTAAC
HindIII 190 AAGCTT A^AGCTT
4379 AAGCTT
KpnI 128 GGTACC GGTAC^C
582 GGTACC
PflMI 1407 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4249 CCAGCCTTTGG
RcaI 1068 TCATGA T^CATGA
1152 TCATGA
SacI 3710 GAGCTC GAGCT^C
3907 GAGCTC
SexAI 1092 ACCTGGT A^CC[AT]GGT
2520 ACCAGGT
SmlI 4382 CTTGAG C^T[CT][GA]AG
4389 CTCAAG
SpeI 3684 ACTAGT A^CTAGT
5253 ACTAGT
Van91I 1407 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4249 CCAGCCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AciI AcyI AfaI AflIII AhdI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI Asp700I AspEI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI Eam1105I EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI XmnI