##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AclI AgeI AocI AscI
AseI AsnI AviII AvrII BbeI
BciVI BlnI BsaI BsiWI BspDI
BsrDI BstBI Bsu36I ClaI CpoI
DrdI Eco81I EcoRV FspI HpaI
KasI MfeI MunI NarI NdeI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1020 GGTACC G^GTACC
AccIII 308 TCCGGA T^CCGGA
AhdI 2775 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 951 AGCGCT AGC^GCT
Asp718I 1020 GGTACC G^GTACC
AspEI 2775 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 125 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2150 CCTCAGC CCTCAGC(-5/-2)
BcgI 452 GCAGGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1047 TGATCA T^GATCA
BglII 1512 AGATCT A^GATCT
BsaBI 478 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 308 TCCGGA [AT]^CCGG[AT]
BseAI 308 TCCGGA T^CCGGA
BsgI 1382 GTGCAG GTGCAG(16/14)
BspEI 308 TCCGGA T^CCGGA
BspLU11I 3590 ACATGT A^CATGT
BssHII 58 GCGCGC G^CGCGC
Bst1107I 3594 GTATAC GTA^TAC
BstAPI 4314 GCATGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3594 GTATAC GTA^TAC
Eam1105I 2775 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 951 AGCGCT AGC^GCT
FbaI 1047 TGATCA T^GATCA
FseI 1156 GGCCGGCC GGCCGG^CC
HaeII 951 AGCGCT [GA]GCGC^[CT]
HincII 4761 GTTGAC GT[CT]^[GA]AC
HindII 4761 GTTGAC GT[CT]^[GA]AC
KpnI 1020 GGTACC GGTAC^C
KspI 104 CCGCGG CCGC^GG
MamI 478 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 308 TCCGGA T^CCGGA
NheI 650 GCTAGC G^CTAGC
PflFI 125 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2893 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 740 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 104 CCGCGG CCGC^GG
SapI 272 GAAGAGC GCTCTTC(1/4)
SexAI 1563 ACCAGGT A^CC[AT]GGT
SgrAI 1131 CGCCGGTG C[GA]^CCGG[CT]G
Tth111I 125 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 3594 GTATAC GT^[AC][GT]AC
5559 GTCTAC
AcyI 128 GGCGTC G[GA]^CG[CT]C
2357 GACGCC
AflII 1544 CTTAAG C^TTAAG
3063 CTTAAG
Asp700I 4397 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5389 GAAATATTTC
BamHI 1092 GGATCC G^GATCC
1539 GGATCC
BbrPI 986 CACGTG CAC^GTG
5477 CACGTG
BfrI 1544 CTTAAG C^TTAAG
3063 CTTAAG
BsaAI 986 CACGTG [CT]AC^GT[GA]
5477 CACGTG
BsaHI 128 GGCGTC G[GA]^CG[CT]C
2357 GACGCC
BspHI 4803 TCATGA T^CATGA
5385 TCATGA
BspMI 2806 ACCTGC ACCTGC(4/8)
3181 ACCTGC
BstEII 2815 GGTGACC G^GT[ACGT]ACC
4538 GGTTACC
BstPI 2815 GGTGACC G^GT[ACGT]ACC
4538 GGTTACC
EcoO65I 2815 GGTGACC G^GT[ACGT]ACC
4538 GGTTACC
EcoRI 3516 GAATTC G^AATTC
3992 GAATTC
EcoT22I 4134 ATGCAT ATGCA^T
5080 ATGCAT
Hin1I 128 GGCGTC G[GA]^CG[CT]C
2357 GACGCC
MluI 3598 ACGCGT A^CGCGT
5146 ACGCGT
NsiI 4134 ATGCAT ATGCA^T
5080 ATGCAT
PmaCI 986 CACGTG CAC^GTG
5477 CACGTG
PmlI 986 CACGTG CAC^GTG
5477 CACGTG
RcaI 4803 TCATGA T^CATGA
5385 TCATGA
SfiI 568 GGCCGGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2446 GGCCCTCATGGCC
SmaI 68 CCCGGG CCC^GGG
2710 CCCGGG
XcmI 3478 CCATGCCACAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4772 CCATGGAACTTCTGG
XmaCI 68 CCCGGG C^CCGGG
2710 CCCGGG
XmaI 68 CCCGGG C^CCGGG
2710 CCCGGG
XmnI 4397 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5389 GAAATATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BsrBI BsrFI BsrGI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII