################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII AclI AgeI AocI AscI AseI AsnI AviII AvrII BbeI BciVI BlnI BsaI BsiWI BspDI BsrDI BstBI Bsu36I ClaI CpoI DrdI Eco81I EcoRV FspI HpaI KasI MfeI MunI NarI NdeI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SfoI SfuI SnaBI SpeI SphI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1020 GGTACC G^GTACC AccIII 308 TCCGGA T^CCGGA AhdI 2775 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 951 AGCGCT AGC^GCT Asp718I 1020 GGTACC G^GTACC AspEI 2775 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 125 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 2150 CCTCAGC CCTCAGC(-5/-2) BcgI 452 GCAGGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1047 TGATCA T^GATCA BglII 1512 AGATCT A^GATCT BsaBI 478 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 308 TCCGGA [AT]^CCGG[AT] BseAI 308 TCCGGA T^CCGGA BsgI 1382 GTGCAG GTGCAG(16/14) BspEI 308 TCCGGA T^CCGGA BspLU11I 3590 ACATGT A^CATGT BssHII 58 GCGCGC G^CGCGC Bst1107I 3594 GTATAC GTA^TAC BstAPI 4314 GCATGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 3594 GTATAC GTA^TAC Eam1105I 2775 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 951 AGCGCT AGC^GCT FbaI 1047 TGATCA T^GATCA FseI 1156 GGCCGGCC GGCCGG^CC HaeII 951 AGCGCT [GA]GCGC^[CT] HincII 4761 GTTGAC GT[CT]^[GA]AC HindII 4761 GTTGAC GT[CT]^[GA]AC KpnI 1020 GGTACC GGTAC^C KspI 104 CCGCGG CCGC^GG MamI 478 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 308 TCCGGA T^CCGGA NheI 650 GCTAGC G^CTAGC PflFI 125 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 2893 AGGACCT [GA]G^G[AT]CC[CT] PshAI 740 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 104 CCGCGG CCGC^GG SapI 272 GAAGAGC GCTCTTC(1/4) SexAI 1563 ACCAGGT A^CC[AT]GGT SgrAI 1131 CGCCGGTG C[GA]^CCGG[CT]G Tth111I 125 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 3594 GTATAC GT^[AC][GT]AC 5559 GTCTAC AcyI 128 GGCGTC G[GA]^CG[CT]C 2357 GACGCC AflII 1544 CTTAAG C^TTAAG 3063 CTTAAG Asp700I 4397 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5389 GAAATATTTC BamHI 1092 GGATCC G^GATCC 1539 GGATCC BbrPI 986 CACGTG CAC^GTG 5477 CACGTG BfrI 1544 CTTAAG C^TTAAG 3063 CTTAAG BsaAI 986 CACGTG [CT]AC^GT[GA] 5477 CACGTG BsaHI 128 GGCGTC G[GA]^CG[CT]C 2357 GACGCC BspHI 4803 TCATGA T^CATGA 5385 TCATGA BspMI 2806 ACCTGC ACCTGC(4/8) 3181 ACCTGC BstEII 2815 GGTGACC G^GT[ACGT]ACC 4538 GGTTACC BstPI 2815 GGTGACC G^GT[ACGT]ACC 4538 GGTTACC EcoO65I 2815 GGTGACC G^GT[ACGT]ACC 4538 GGTTACC EcoRI 3516 GAATTC G^AATTC 3992 GAATTC EcoT22I 4134 ATGCAT ATGCA^T 5080 ATGCAT Hin1I 128 GGCGTC G[GA]^CG[CT]C 2357 GACGCC MluI 3598 ACGCGT A^CGCGT 5146 ACGCGT NsiI 4134 ATGCAT ATGCA^T 5080 ATGCAT PmaCI 986 CACGTG CAC^GTG 5477 CACGTG PmlI 986 CACGTG CAC^GTG 5477 CACGTG RcaI 4803 TCATGA T^CATGA 5385 TCATGA SfiI 568 GGCCGGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2446 GGCCCTCATGGCC SmaI 68 CCCGGG CCC^GGG 2710 CCCGGG XcmI 3478 CCATGCCACAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4772 CCATGGAACTTCTGG XmaCI 68 CCCGGG C^CCGGG 2710 CCCGGG XmaI 68 CCCGGG C^CCGGG 2710 CCCGGG XmnI 4397 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5389 GAAATATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrBI BsrFI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII