##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AgeI AocI AscI
AseI AsnI AspI AviII AvrII
BbeI BciVI BlnI BsaI BsiWI
BspDI BsrDI BssHII BstBI Bsu36I
ClaI CpoI DrdI Eco81I EcoRV
FspI HpaI KasI KspI MfeI
MunI NarI NdeI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 878 GGTACC G^GTACC
AccIII 166 TCCGGA T^CCGGA
AhdI 2633 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 809 AGCGCT AGC^GCT
Asp718I 878 GGTACC G^GTACC
AspEI 2633 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 2008 CCTCAGC CCTCAGC(-5/-2)
BcgI 310 GCAGGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 905 TGATCA T^GATCA
BglII 1370 AGATCT A^GATCT
BsaBI 336 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 166 TCCGGA [AT]^CCGG[AT]
BseAI 166 TCCGGA T^CCGGA
BsgI 1240 GTGCAG GTGCAG(16/14)
BspEI 166 TCCGGA T^CCGGA
BspLU11I 3448 ACATGT A^CATGT
Bst1107I 3452 GTATAC GTA^TAC
BstAPI 4172 GCATGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3452 GTATAC GTA^TAC
Eam1105I 2633 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 809 AGCGCT AGC^GCT
FbaI 905 TGATCA T^GATCA
FseI 1014 GGCCGGCC GGCCGG^CC
HaeII 809 AGCGCT [GA]GCGC^[CT]
HincII 4619 GTTGAC GT[CT]^[GA]AC
HindII 4619 GTTGAC GT[CT]^[GA]AC
KpnI 878 GGTACC GGTAC^C
MamI 336 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 166 TCCGGA T^CCGGA
NheI 508 GCTAGC G^CTAGC
PpuMI 2751 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 598 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 130 GAAGAGC GCTCTTC(1/4)
SexAI 1421 ACCAGGT A^CC[AT]GGT
SgrAI 989 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 2568 CCCGGG CCC^GGG
XmaCI 2568 CCCGGG C^CCGGG
XmaI 2568 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3452 GTATAC GT^[AC][GT]AC
5417 GTCTAC
AcyI 40 GACGCC G[GA]^CG[CT]C
2215 GACGCC
AflII 1402 CTTAAG C^TTAAG
2921 CTTAAG
Asp700I 4255 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5247 GAAATATTTC
BamHI 950 GGATCC G^GATCC
1397 GGATCC
BbrPI 844 CACGTG CAC^GTG
5335 CACGTG
BfrI 1402 CTTAAG C^TTAAG
2921 CTTAAG
BsaAI 844 CACGTG [CT]AC^GT[GA]
5335 CACGTG
BsaHI 40 GACGCC G[GA]^CG[CT]C
2215 GACGCC
BspHI 4661 TCATGA T^CATGA
5243 TCATGA
BspMI 2664 ACCTGC ACCTGC(4/8)
3039 ACCTGC
BstEII 2673 GGTGACC G^GT[ACGT]ACC
4396 GGTTACC
BstPI 2673 GGTGACC G^GT[ACGT]ACC
4396 GGTTACC
EcoNI 675 CCTCGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3205 CCTTTTGAAGG
EcoO65I 2673 GGTGACC G^GT[ACGT]ACC
4396 GGTTACC
EcoRI 3374 GAATTC G^AATTC
3850 GAATTC
EcoT22I 3992 ATGCAT ATGCA^T
4938 ATGCAT
Hin1I 40 GACGCC G[GA]^CG[CT]C
2215 GACGCC
MluI 3456 ACGCGT A^CGCGT
5004 ACGCGT
NsiI 3992 ATGCAT ATGCA^T
4938 ATGCAT
PmaCI 844 CACGTG CAC^GTG
5335 CACGTG
PmlI 844 CACGTG CAC^GTG
5335 CACGTG
RcaI 4661 TCATGA T^CATGA
5243 TCATGA
SfiI 426 GGCCGGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2304 GGCCCTCATGGCC
XcmI 3336 CCATGCCACAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4630 CCATGGAACTTCTGG
XmnI 4255 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5247 GAAATATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BsrBI BsrFI BsrGI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII