##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AclI AflII AgeI AscI BamHI
BcgI BfrI BsiWI BsmBI BsmI
BspDI BsrDI BssHII BstBI ClaI
CpoI EcoRV EcoT22I FseI HincII
HindII HpaI MfeI MluI MunI
NotI NsiI NspV PinAI PmeI
PshAI Psp1406I PvuI RsrII SalI
SapI ScaI SfuI SgrAI SnaBI
SpeI SwaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 3773 GACGTC GACGT^C
AccI 4055 GTATAC GT^[AC][GT]AC
AccIII 30 TCCGGA T^CCGGA
AflIII 5513 ACATGT A^C[GA][CT]GT
AseI 675 ATTAAT AT^TAAT
AsnI 675 ATTAAT AT^TAAT
AspI 4557 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 147 GGCGCC GGCGC^C
BbrPI 4386 CACGTG CAC^GTG
BbsI 644 GAAGAC GAAGAC(2/6)
BciVI 1814 GTATCC GTATCC(6/5)
BglII 1289 AGATCT A^GATCT
BpuAI 644 GAAGAC GAAGAC(2/6)
BsaAI 4386 CACGTG [CT]AC^GT[GA]
BsaBI 2419 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 30 TCCGGA T^CCGGA
BsiEI 140 CGGCCG CG[GA][CT]^CG
Bsp1407I 3344 TGTACA T^GTACA
BspEI 30 TCCGGA T^CCGGA
BspHI 5061 TCATGA T^CATGA
BspLU11I 5513 ACATGT A^CATGT
BsrBI 710 GAGCGG CCGCTC(-3/-3)
BsrGI 3344 TGTACA T^GTACA
Bst1107I 4055 GTATAC GTA^TAC
BstAPI 2901 GCACCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4055 GTATAC GTA^TAC
DrdI 3143 GACAGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 140 CGGCCG C^GGCCG
EclXI 140 CGGCCG C^GGCCG
Eco52I 140 CGGCCG C^GGCCG
EcoRI 4284 GAATTC G^AATTC
KasI 147 GGCGCC G^GCGCC
MamI 2419 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 30 TCCGGA T^CCGGA
NaeI 165 GCCGGC GCC^GGC
NarI 147 GGCGCC GG^CGCC
NcoI 3084 CCATGG C^CATGG
NdeI 4083 CATATG CA^TATG
NgoMIV 165 GCCGGC G^CCGGC
NheI 2774 GCTAGC G^CTAGC
NruI 377 TCGCGA TCG^CGA
PacI 672 TTAATTAA TTAAT^TAA
PaeR7I 3472 CTCGAG C^TCGAG
PflFI 4557 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4386 CACGTG CAC^GTG
PmlI 4386 CACGTG CAC^GTG
PshBI 675 ATTAAT AT^TAAT
RcaI 5061 TCATGA T^CATGA
SacI 4788 GAGCTC GAGCT^C
SbfI 3205 CCTGCAGG CCTGCA^GG
SfoI 147 GGCGCC GGC^GCC
SphI 2092 GCATGC GCATG^C
Sse8387I 3205 CCTGCAGG CCTGCA^GG
SspBI 3344 TGTACA T^GTACA
Tth111I 4557 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3472 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 2274 GGTACC G^GTACC
3534 GGTACC
AocI 2178 CCTCAGG CC^T[ACGT]AGG
4687 CCTGAGG
Aor51HI 85 AGCGCT AGC^GCT
2616 AGCGCT
Asp700I 465 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1873 GAATGGATTC
Asp718I 2274 GGTACC G^GTACC
3534 GGTACC
AviII 4397 TGCGCA TGC^GCA
5305 TGCGCA
AvrII 2442 CCTAGG C^CTAGG
4000 CCTAGG
BbvCI 3172 CCTCAGC CCTCAGC(-5/-2)
3500 GCTGAGG
BclI 5093 TGATCA T^GATCA
5354 TGATCA
BlnI 2442 CCTAGG C^CTAGG
4000 CCTAGG
BsaI 2162 GGTCTC GGTCTC(1/5)
5126 GGTCTC
BsaWI 30 TCCGGA [AT]^CCGG[AT]
2271 TCCGGT
BsrFI 165 GCCGGC [GA]^CCGG[CT]
3026 ACCGGC
BssSI 765 CTCGTG CACGAG(-5/-1)
882 CTCGTG
BstEII 683 GGTCACC G^GT[ACGT]ACC
3733 GGTGACC
BstPI 683 GGTCACC G^GT[ACGT]ACC
3733 GGTGACC
Bsu36I 2178 CCTCAGG CC^T[ACGT]AGG
4687 CCTGAGG
Cfr10I 165 GCCGGC [GA]^CCGG[CT]
3026 ACCGGC
DraI 1320 TTTAAA TTT^AAA
2793 TTTAAA
Eco47III 85 AGCGCT AGC^GCT
2616 AGCGCT
Eco81I 2178 CCTCAGG CC^T[ACGT]AGG
4687 CCTGAGG
EcoO65I 683 GGTCACC G^GT[ACGT]ACC
3733 GGTGACC
FbaI 5093 TGATCA T^GATCA
5354 TGATCA
FspI 4397 TGCGCA TGC^GCA
5305 TGCGCA
HindIII 2358 AAGCTT A^AGCTT
3660 AAGCTT
KpnI 2274 GGTACC GGTAC^C
3534 GGTACC
SexAI 593 ACCTGGT A^CC[AT]GGT
3537 ACCTGGT
SfiI 1798 GGCCATGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3203 GGCCTGCAGGGCC
SmaI 40 CCCGGG CCC^GGG
2040 CCCGGG
XbaI 2621 TCTAGA T^CTAGA
3281 TCTAGA
XmaCI 40 CCCGGG C^CCGGG
2040 CCCGGG
XmaI 40 CCCGGG C^CCGGG
2040 CCCGGG
XmnI 465 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1873 GAATGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII